The I.M.A.G.E. Consortium

"Sharing resources to achieve a common goal - the discovery of all genes"

Vector List

Note: The sequencing primers listed below for each vector are not exhaustive (only the most commonly used primers have been checked, -21M13, M13 reverse, t7, t3, and sp6) and others not listed may also be suitable. We also recommend that you check the exact sequence of your primer against the vector sequence to ensure that it matches, as there are often several slightly different versions of these common primers. When custom primers are required, their sequences are listed.

Lafmid BA

NAME: Lafmid BA (ampicillin resistant; 50-200 ug/ml)
SOURCE: Columbia University/Soares
V_TYPE: phagemid
POLYLINKER SEQUENCE:
taacaatttcacacaggaaacagctatgaccatgattacgccaagcttgg
ctcgacggatccccttgcggccgcaaggggaattccactggccgtcgttt
tacaacgtcgtgactgggaaaaccc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse
||

pAD-GAL4

NAME: pAD-GAL4 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ccaaacccaaaaaaagagatcgaattcgcccgggcctcgagcccgggtcg
actctagagccctatagtgagtcgtattactgcagagatctatgaatcgt
agatactgaaaaac
FULL SEQUENCE: not available
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7
||

pAMP1

NAME: pAMP1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
gattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccagtgaattgaatttaggtgacactatagaagagctatgacgtcg
catgcacgcgtacgtaagcttggatcctctagagcggccgcctactacta
ctaatgatgatgatgcccgggaattccggaccggtacctgcaggcgtacc
agctttccctatagtgagtcgtattagagcttggcgtaatcatggtcata
gctgtttcctgtgtgaaattgttatccgctcacaattccacaca
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pAMP10

NAME: pAMP10 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
cccagtcacgacgttgtaaaacgacggccagtgaattgaatttaggtgac
actatagaagagctatgacgtcgcatgcacgcgtacgtaagcttggatcc
tctagagcggccggccgcctactactactaatgatgatgatgtcgacccg
ggaattccggaccggtacctgcaggcgtaccagctttccctatagtgagt
cgtattagagcttggcgtaatcatggtcatagctgtttcctgtgtgaaat
tgttatc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pBC SK+

NAME: pBC SK+ (chloramphenicol resistant; 25 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCG
AATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAT
ATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCAC
CGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGC
TTGGCGTAATCATGGTCATAGCTGTTTCC
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBK-CMV

NAME: pBK-CMV (kanamycin resistant; 25 ug/ml)
SOURCE: Stratagene
V_TYPE: plasmid
POLYLINKER SEQUENCE:
aaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttc
ccagtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcac
tatagggcgaattgggtacacttacctggtaccccacccgggtggaaaat
cgatgggcccgcggccgctctagaagtactctcgagaagctttttgaatt
ctttggatccactagtgtcgacctgcaggcgcgcgagctccagcttttgt
tccctttagtgagggttaatttcgagcttggcgtaatcaaggtcatagct
gtttcctgtgtgaaattgttatccgctcacaattccacacaatatacgag
ccggaagtataaagtgtaaagcctggggtgccta
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescribe (modified)

NAME: pBluescribe (modified) (ampicillin resistant; 50-200 ug/ml)
SOURCE: Jackson Laboratory
V_TYPE: phagemid
POLYLINKER SEQUENCE:
taatacgactcactatagggatcactatagggcgaattgacgcgtg...5
' end of insert (Knowles/Solter libraries)........
.3' end of insert...gtcgaccggagcttttgttccctttagtga
gggttaat
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript (modified)

NAME: pBluescript (modified) (ampicillin resistant; 50-200 ug/ml)
SOURCE: UCLA/Lin
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcg
aattgggtaccgggccccccctcgaggtcgacggtatcgataagcttgat
atcgaattccactgcgtgctgcgcccgggggatccgcatgccacactgtg
tctagagcggccgccaccgcggtggagctccagttttgttccctttagtg
agggttaattgcgcgcttggcgtaatcatggtcatagctgtttcc
FULL SEQUENCE: not available
VECTOR MAP: not available
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript II KS+

NAME: pBluescript II KS+ (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ggaaacagctatgaccatgattacgccaagctcgaaattaaccctcacta
aagggaacaaaagctggagctccaccgcggtggcggccgctctagaacta
gtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtatt
acaattcactggccgtcgttttacaa
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript II SK+

NAME: pBluescript II SK+ (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
GCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCT
ACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGC
GATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGT
GCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACG
TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCG
AATTGGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTGAT
ATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCAC
CGCGGTGGAGCTCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGC
TTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCT
CACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGG
GTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCC
GCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCA
ACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGC
TCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCT
CACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGC
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript KS+

NAME: pBluescript KS+ (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
CGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGGGGATGTGCT
GCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAAT
TGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCCCGGG
CTGCAGGAATTCGATATCAAGCTTATCGATACCGTCGACCTCGAGGGGGG
GCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTG
GCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCAC
AATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
CCTAATGAGTGAGCTA
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript SK+

NAME: pBluescript SK+ (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ggaaacagctatgaccatgattacgccaagctcgaaattaaccctcacta
aagggaacaaaagctggagctccaccgcggtggcggccgctctagaacta
gtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtatt
acaattcactggccgtcgttttacaa
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript SK-

NAME: pBluescript SK- (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ggaaacagctatgaccatgattacgccaagctcgaaattaaccctcacta
aagggaacaaaagctggagctccaccgcggtggcggccgctctagaacta
gtggatcccccgggctgcaggaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtatt
acaattcactggccgtcgttttacaa
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBluescript-FL

NAME: pBluescript-FL (ampicillin resistant; 50-200 ug/ml)
SOURCE: University of Tokyo/Sugano lab
V_TYPE: phagemid
POLYLINKER SEQUENCE:
actagtggatccattgtttggccttgttggccatcatttgggctgcag
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, t3
||

pBluescriptR

NAME: pBluescriptR (ampicillin resistant; 50-200 ug/ml)
SOURCE: Brownstein
V_TYPE: phagemid
POLYLINKER SEQUENCE:
tgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaa
agggggatgtgctgcaaggcgattaagttgggtaacgccagggttttccc
agtcacgacgttgtaaaacgacggccagtgaattgtaatacgactcacta
tagggcgaattggagctccaccgcggtggcggccgcataacttcgtatag
catacattatacgaagttatggatcaggccaaatcggccgagctcgaatt
cgtcgag----5'-insert-3'----ggatccggccataagggcctgat
ccgtcgagggggggcccggtaccagcttttgttccctttagtgagggtta
atttcgagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattg
ttatccgctcacaattccacacaacatacgagccggaagcataaagtgta
aagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgc
tcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatg
aatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccg
cttcctcgctcactgactcgctgcgctcgg
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pBSRN3

NAME: pBSRN3 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Wellcome Trust
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gagctaagcttgcttgttctttttgcagaagctcagaataaacgctcaac
tttggcagatctgaattccccggggcggccgcggatctggttaccactaa
accagcctcaagaacacccgaatggagtctctaagctacataataccaac
ttacactttacaaaatgttgtcccccaaaatgtagccattcgtatctgct
cctaataaaaagaaagtttcttcacttctaaaaa
FULL SEQUENCE: not available
VECTOR MAP: not available
SEQUENCING PRIMERS: custom
||

pCDNA3

NAME: pCDNA3 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTA
CGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTT
ACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGAC
GTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATT
GACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACAT
CAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAA
ATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTA
CTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGG
TTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATT
TCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAA
ATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAA
ATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTG
GCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTC
ACTATAGGGAGACCCAAGCTTGGTACCGAGCTCGGATCCACTAGTAACGG
CCGCCAGTGTGCTGGAATTCTGCAGATATCCATCACACTGGCGGCCGCTC
GAGCATGCATCTAGAGGGCCCTATTCTATAGTGTCACCTAAATGCTAGAG
CTCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTT
TGCCCCTCCCCCGTGCC
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7
||

pcDNA3.1

NAME: pcDNA3.1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
agtacatcaatgggcgtggatagcggtttgactcacggggatttccaagt
ctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacg
ggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcg
gtaggcgtgtacggtgggaggtctatataagcagagctctctggctaact
agagaacccactgcttactggcttatcgaaattaatacgactcactatag
ggagacccaagcttggtaccgagctcggatccactagtaacggccgccag
tgtgctggaattctgcagatatccatcacactggcggccgctcgagcatg
catctagagggccctattctatagtgtcacctaaatgctagagctcgctg
atcagcctcgactgtgcctctagttgccagccatctgttgtttgcccctc
ccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcct
aataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctatt
ctggggggtggggtggggcaggacagcaagggggaggattgggaagacaa
tagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaa
gaaccagctggggctctagggggtatccccacgcgccctgtagcggcgca
ttaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgc
cagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgcca
cgttcgccggctttccccgtcaagctctaaatcggggca
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, sp6
||

pcDNA3.1(-)

NAME: pcDNA3.1(-) (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
atgtacgggccagatatacgcgttgacattgattattgactagttattaa
tagtaatcaattacggggtcattagttcatagcccatatatggagttccg
cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacc
cccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaata
gggactttccattgacgtcaatgggtggagtatttacggtaaactgccca
cttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacg
tcaatgacggtaaatggcccgcctggcattatgcccagtacatgacctta
tgggactttcctacttggcagtacatctacgtattagtcatcgctattac
catggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttg
actcacggggatttccaagtctccaccccattgacgtcaatgggagtttg
ttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgc
cccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataa
gcagagctctctggctaactagagaacccactgcttactggcttatcgaa
attaatacgactcactatagggagacccaagctggctagcgtttaaacgg
gccctctagactcgagcggccgccactgtgctggatatctgcagaattcc
accacactggactagtggatccgagctcggtaccaagcttaagtttaaac
cgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttg
cccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcc
tttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcat
tctattctggggggtggggtggggcaggacagcaagggggaggattggga
agacaatagcaggcatgctggggatgcggtgggctctatggcttctgagg
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 reverse, t7
||

pcDNA3.1Zeo

NAME: pcDNA3.1Zeo (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagt
aacgccaatagggactttccattgacgtcaatgggtggactatttacggt
aaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccc
cctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta
catgaccttatgggactttcctacttggcagtacatctacgtattagtca
tcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtgga
tagcggtttgactcacggggatttccaagtctccaccccattgacgtcaa
tgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgta
acaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggag
gtctatataagcagagctctctggctaactagagaacccactgcttactg
gcttatcgaaattaatacgactcactatagggagacccaagctggctagc
gtttaaacttaagcttggtaccgagctcggatccactagtccagtgtggt
ggaattctgcagatatccagcacagtggcggccgctcgagtctagagggc
ccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagcca
tctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccac
tcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctga
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 reverse, t7
||

pcDNAI

NAME: pcDNAI (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaa
tgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacg
gtgggaggtctatataagcagagctctctggctaactagagaacccactg
cttactggcttatcgaaattaatacgactcactatagggagacccaagct
tggtaccgagctcggatccactagtaacggccgccagtgtgctggaattc
tgcagatatccatcacactggcggccgctcgagcatgcatctagagggcc
ctattctatagtgtcacctaaatgctagaggatctttgtgaaggaacctt
acttctgtggtgtgacataattggacaaactacctacagagatttaaagc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, sp6
||

pCDNAII

NAME: pCDNAII (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
ccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgc
cagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgtaa
tacgactcactatagggcgaattgggccctctagatgcatgctcgagcgg
ccgccagtgtgatggatatctgcagaattccagcacactggcggccgtta
ctagtggatccgagctcggtaccaagcttgatgcatagcttgagtattct
atagtgtcacctaaatagcttggcgtaatcatggtcatagctgtttcctg
tgtgaaattgtt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pCI-neo (updated)

NAME: pCI-neo (updated) (ampicillin resistant; 50-200 ug/ml)
SOURCE: McArdle Laboratory for Cancer Research
V_TYPE: plasmid
POLYLINKER SEQUENCE:
tgcagaagttggtcgtgaggcactgggcaggtaagtatcaaggttacaag
acaggtttaaggagaccaatagaaactgggcttgtcgagacagagaagac
tcttgcgtttctgataggcacctattggtcttactgacatgtccactccc
agttcaattacagctcttaaggctagagtacttaatacgactcactatag
gctagcggcgcgccgaattcgtcgacttaattaagcggccgcttcccttt
agtgagggttaatgcttcgagcagacatgataagatacattgatgagttt
ggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaat
ttgtgatgctattgctttatttgtaaccattataagctgcaataaacaag
ttaacaacaacaattgcattcattttatgtttcaggttcagggggagatg
tgggaggttttttaaagcaagt
FULL SEQUENCE: not available
VECTOR MAP: not available
SEQUENCING PRIMERS: t3, custom t7
||

pCMV-SPORT

NAME: pCMV-SPORT (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
taatacgactcactatagggagagagctatgacgtcgcatgcacgcgtaa
gcttgggcccctcgagggatcctctagagcggccgc{3' - cDNA ins
ert - 5'}cggACGCGTgggtcgacccgggaattccggaccggtacctg
caggcgtaccttctatagtgtcacctaaatagctttttgcaaaagcctag
gctagagtccggagg{CMV Promoter}
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, sp6
||

pCMV-SPORT2

NAME: pCMV-SPORT2 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
gacgttgtaaaacgacggccagtgcctagcttataatacgactcactata
gggagagagctatgacgtcgcatgcacgcgtaagcttgggcccctcgagg
gatcctctagagcggccgc{3' - cDNA insert - 5'}cggACGCG
Tgggtcgacccgggaattccggaccggtacctgcaggcgtaccttctata
gtgtcacctaaatagctttttgcaaaagcctagtggtcatagctgtttcc
tgtgtgaaattgttatccgctccgcggcctaggctagagtcc{CMV Pro
moter}
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pCMV-SPORT4

NAME: pCMV-SPORT4 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
gacgttgtaaaacgacggccagtgcctagcttataatacgactcactata
gggagagagctatgacgtcgcatgcacgcgtaagcttgggcccctcgagg
gatcctctagagcggccgc{3' - cDNA insert - 5'}cggACGCG
Tgggtcgacccgggaattccggaccggtacctgcaggcgtaccttctata
gtgtcacctaaatagctttttgcaaaagcctagtggtcatagctgtttcc
tgtgtgaaattgttatccgctccgcggcctaggctagagtcc{CMV Pro
moter}
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pCMV-SPORT6

NAME: pCMV-SPORT6 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
gatcccaagctagcagttttcccagtcacgacgttgtaaaacgacggcca
gtgcctagcttataatacgactcactatagggaccactttgtacaagaaa
gctgggtacgcgtaagcttgggcccctcgagggatactctagagcggccg
ccgactagtgagctcgtcgacgatatcccgggaattccggaccggtacca
gcctgcttttttgtacaaacttgttctatagtgtcacctaaataggccta
atggtcatagctgtttcctgtgtgaaatt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pCMV-SPORT6.1

NAME: pCMV-SPORT6.1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
caccacagaagtaaggttccttcacaaagatcccaagctagcagttttcc
cagtcacgacgttgtaaaacgacggccagtgcctagcttataatacgact
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatactctagagcggccgccgatccggcttactaaaagcc
agataacagtatgcgtatttgcgcgctgatttttgcggtataagaatata
tactgatatgtatacccgaagtatgtcaaaaagaggtgtgcttctagaat
gcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttg
tggatgtacagagtgatattattgacacgcccgggcgacggatggtgatc
cccctggccagtgcacgtctgctgtcagataaagtctcccgtgaacttta
cccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgata
tggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagc
caccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaat
ataagtcgacgatatcccgggaattccggaccggtaccagcctgcttttt
tgtacaaacttgttctatagtgtcacctaaataggcctaatggtcatagc
tgtttcctgtgtgaaattgttatccgctccgcggcctaggctagagtccg
gaggctggatcggtcccggtgtcttctatggaggtcaaaacag
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, sp6
||

pCMV-SPORT6.ccdb

NAME: pCMV-SPORT6.ccdb (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
cactatagggaccactttgtacaagaaagctgggtacgcgtaagcttggg
cccctcgagggatcctctagagcggccgccgatccggcttactaaaagcc
agataacagtatgcgtatttgcgcgctgatttttgcggtataagaatata
tactgatatgtatacccgaagtatgtcaaaaagaggtgtgcttctagaat
gcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttg
tggatgtacagagtgatattattgacacgcccgggcgacggatggtgatc
cccctggccagtgcacgtctgctgtcagataaagtctcccgtgaacttta
cccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgata
tggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagc
caccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaat
ataagtcgacgatatcccgggaattccggaccggtaccagcctgcttttt
tgtacaaacttgttctatagtgtcacctaaataggcctaatggtcatagc
tgtttcctgtgtgaaattgttatccgctccgcggcctaggctagagtccg
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pCR-BluntII-TOPO

NAME: pCR-BluntII-TOPO (kanamycin resistant; 10ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
acagctatgaccatgattacgccaagctatttaggtgacactatagaata
ctcaagctatgcatcaagcttggtaccgagctcggatccactagtaacgg
ccgccagtgtgctggaattcgcccttaagggcgaattctgcagatatcca
tcacactggcggccgctcgagcatgcatctagagggcccaattcgcccta
tagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgact
gggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccct
ttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca
acagttgcgcagcctatacgtacggcagtttaaggtttacacctataaaa
gagagagccgttatcgtctgtttgtggatgtacagagtgatattattgac
acgccggggcgacggatggtgatccccctggccagtgcacgtctgctgtc
agataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaa
gctggcgcat
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pCR-XL-TOPO

NAME: pCR-XL-TOPO (kanamycin resistant; 25 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
CACACAGGAAACAGCTATGACCATGATTACGCCAAGCTATTTAGGTGACG
CGTTAGAATACTCAAGCTATGCATCAAGCTTGGTACCGAGCTCGGATCCA
CTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTAAGGGCGAATTCTG
CAGATATCCATCACACTGGCGGCCGCTCGAGCATGCATCTAGAGGGCCCA
ATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAA
CGTCGTGACTGGGAAAACCCTGGCGT
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7
||

pCR2.1-TOPO

NAME: pCR2.1-TOPO (ampicillin resistant; 100 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cacacaggaaacagctatgaccatgattacgccaagcttggtaccgagct
cggatccactagtaacggccgccagtgtgctggaattcgcccttaagggc
gaattctgcagatatccatcacactggcggccgctcgagcatgcatctag
agggcccaattcgccctatagtgagtcgtattacaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
cttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccg
caccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacg
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7
||

pCR3.1

NAME: pCR3.1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
tcgaaattaatacgactcactatagggagacccaagctggctagcgttta
aacttaagcttggtaccgagctcggatccactagtccagtgtggtggaat
tcggcttaagccgaattctgcagatatccagcacagtggcggccgctcga
gtctagagggcccgtttaaacccgctgatcagcctcgactgtgccttcta
gttgccagcc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7
||

pCR4-TOPO

NAME: pCR4-TOPO (kanamycin resistant; 25 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
GCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCAC
GACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGT
GAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGG
CTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAAC
AGCTATGACCATGATTACGCCAAGCTCAGAATTAACCCTCACTAAAGGGA
CTAGTCCTGCAGGTTTAAACGAATTCGCCCTTAAGGGCGAATTCGCGGCC
GCTAAATTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGT
CGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATC
GCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCC
CGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTATACGTACGGCAGTT
TAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATG
TACAGAGTGATATTATTGACACGCCGGGGCGACGGATGGTGATCCCCCTG
GCCAGTGCACGTCTGCTG
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pCR4Blunt-TOPO

NAME: pCR4Blunt-TOPO (ampicillin resistant and kanamycin resistant; 25 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 reverse, M13 -20
||

pCS105

NAME: pCS105 (ampicillin resistant; 50-200 ug/ml)
SOURCE: UCBerkeley/Harland
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattcg
tcgacaggccaatctggccgcggccgcaaggcctctcgagcctctagaac
tatagtgagtcgtattacgtagatccagacatgataagatacattgatga
gtttggacaaaccacaactagaatgcagtgaaaaaaatgc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: sp6
||

pCS107

NAME: pCS107 (ampicillin resistant; 50-200 ug/ml)
SOURCE: UCBerkeley/Harland
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattcg
tcgacaggccaatctggccgcggccgcaaggcctctcgagcctctgcggc
tatagtgagtcgtattacgtagatccagacatgataagatacattgatga
gtttggacaaaccacaactagaatgcagtgaaaaaaatgc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, sp6
||

pCS108

NAME: pCS108 (ampicillin resistant; 50-200 ug/ml)
SOURCE: UCBerkeley/Harland
V_TYPE: plasmid
POLYLINKER SEQUENCE:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGACACTAT
AGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCGAATTCG
TCGACAGGCCAATCTGGCCGCGGCCGCAAGGCCTGATATCTCTAGACCGC
GGCTCGAGCCTCTCGCCCTATAGTGAGTCGTATTACGTAGATCCAGACAT
GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA
AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTAT
GTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCGCGCC
GCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGA
GGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTG
AAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAA
AGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCG
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, t3, sp6
||

pCS111

NAME: pCS111 (ampicillin resistant; 50-200 ug/ml)
SOURCE: UCBerkeley/Harland
V_TYPE: plasmid
POLYLINKER SEQUENCE:
TGTGCCAATTCAATATGGCGTATATGGACTCGTGCCAATTCAATATGGTG
GATCTGGACCCCAGCCAATTCAATATGGCTATTGGCCAGGTTCAATACTA
TGTATTGGCCCTATGCCATATAGTATTCCATATATGGGTTTTCCTATTGA
CGTAGATAGCCCCTCCCAATGGGCGGTCCCATATACCATATATGGGGCTT
CCTAATACCGCCCATAGCCACTCCCCCATTGACGTCAATGGTCTCTATAT
ATGGTCTTTCCTATTGACGTCATATGGGCGGTCCTATTGACGTATATGGC
GCCTCCCCCATTGACGTCAATTACGGTAAATGGCCCGCCTGGCTCAATGC
CCATTGACGTCAATAGGACCACCCACCATTGACGTCAATGGGATGGCTCA
TTGCCCATTCATATCCGTTCTCACGCCCCCTATTGACGTCAATGACGGTA
AATGGCCCACTTGGCAGTACATCAATATCTATTAATAGTAACTTGGCAAG
TACATTACTATTGGAAGTACGCCAGGGTACATTGGCAGTACTCCCATTGA
CGTCAATGGCGGTAAATGGCCCGCGATGGCTGCCAAGTACATCCCCATTG
ACGTCAATGGGGAGGGGCAATGACGCAAATGGGCGTTCCATTGACGTAAA
TGGGCGGTAGGCGTGCCTAATGGGAGGTCTATATAAGCAATGCTCGTTTA
GGGAACCGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTGATTTAGGTGA
CACTATAGAATACAAGCTACTTGTTCTTTTTGCAGGATCCCATCGATTCG
AATTCGTCCCGGGAATCTGGCCGCGGCCGCAAGGCCTGATATCTCTAGAC
CGCGGCTCGAGCCTCTCGCCCTATAGTGAGTCGTATTACGTAGATCCAGA
CATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGT
GAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTA
ACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTT
TATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCGC
GCCGCGGCGCCAATGCATTGGGCCCGGTACCCAGCTTTTGTTCCCTTTAG
TGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGT
GTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGGAGCA
TAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATT
GCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCT
GCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGC
GCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTG
CGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGA
TCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGG
FULL SEQUENCE: not available
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, t3, sp6
||

pCS2+

NAME: pCS2+ (ampicillin resistant; 50-200 ug/ml)
SOURCE: Max Planck Institute/Rupp
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cgccattctgcctggggacgtcggagcaagcttgatttaggtgacactat
agaatacaagctacttgttctttttgcaggatcccatcgattcgaattca
aggcctctcgagcctctagaactatagtgagtcgtattacgtagatcgag
a
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, sp6
||

pCS22+

NAME: pCS22+ (ampicillin resistant; 50-200 ug/ml)
SOURCE: UCBerkeley/Harland
V_TYPE: plasmid
POLYLINKER SEQUENCE:
CGCCATTCTGCCTGGGGACGTCGGAGCAAGCTTTAATACGACTCACTATA
GGGCAATACAAGCTACTTGTTTAAACTCTGCAGGGATCCGAATTCCTCGA
GCCCTTTAGTGAGGGTTAATTTACGTAGATCCAGACATGATAAGATACAT
TGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTA
TTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGC
AATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCA
GGGGGAGGTGTGGGAGGTTTTTTAATTCGCGGCCGCGGCGCCAATGCATT
GGGCCCGGTACCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGT
GAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATA
AAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGC
GTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGC
ATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGC
TCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCG
GCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAAT
CAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCC
AGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCC
CCCTGACGAGCATCACAAAA
FULL SEQUENCE: Available here
VECTOR MAP: not available
SEQUENCING PRIMERS
||

pCS2G

NAME: pCS2G (ampicillin resistant; 50-200 ug/ml)
SOURCE: RIKEN/Carninci
V_TYPE: plasmid
POLYLINKER SEQUENCE:
aagcttgatttaggtgacactatagaatacaagctacttgttctttttgc
aggatctctcgaatcacaagtttgtacaaaaaagcaggctctcgagctct
aataaggtgacactatagaacca--------5'-cDNA_insert-3'--
----ctctccagtattggatccacccagctttcttgtacaaagtggtgat
gatccgtccgtcgagcctctacattctgcagccctatagtgagtcgtatt
acgtaga
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, sp6
||

pDNR-Dual

NAME: pDNR-Dual (ampicillin resistant; 50-200 ug/ml)
SOURCE: Clontech
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gcggccgcataacttcgtatagcatacattatacgaagttatcagtcgac
ggtaccggacatatgcccgggaattcctgcaggatccgctcgagaagctt
tctagaccattcgtttggcgcgcgggcccaggtgagtggtcataatcata
atcataatcataatcataatcacaactagcctaggagatcctggtcatga
ctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgt
tctgaacaaatccagatggagttctgaggtcattactggatctatc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), t7
||

pDNR-LIB

NAME: pDNR-LIB (chloramphenicol resistant; 25 ug/ml)
SOURCE: Clontech
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gcggccgcataacttcgtatagcatacattatacgaagttatcagtcgac
ggtaccggacatatgcccgggaattcggccattacggcctgcaggatccg
aaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaa
ttgttgttgttaacttgtttattgcagcttataatggttacaaataaagc
aatagcatcacaaatttcacaaataaagcatttttttcactgcattctag
ttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccggc
cgcctcggccctcgagaagctttctagaccattcgtttggcgcgcgggcc
cagtaggtaagtgaacatggtcatagctgtttccctaggagatcctggta
tgactagtgcttggattctcaccaataaaaaacgcccggcggcaaccgag
cgttctgaacaaatccagatggagttctgaggtcattactggatctatca
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21)
||

pDNR-NCI

NAME: pDNR-NCI (chloramphenicol resistant; 25 ug/ml)
SOURCE: Clontech
V_TYPE: phagemid
POLYLINKER SEQUENCE:
GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTCGAC
GAATTCTGGATCTGGAAACAGCTATGACCATGCTCGGGAAGCGCGCCATT
GTGTTGGTACCCGGGAATTCGGCCATTATGGCCTGCAGGATCCGGTGGTG
GTGCAAATCAAAGAACTGCTCCTCAGTGGATGTTGCCTTTACTTCTAGGC
CTGTACGGAAGTGTTACTTCTGCTCTAAAAGCTGCGGAATTGTACCCGCG
GGCCCACCATGGCATCAATGCAGAAGCTGATCTCAGAGGAGGACCTGCTT
ATGGCCATGGAGGCCCGAATTCGGTCGACCGAGATCTCTCGAGGTACCGC
GGCCGCGGGGATCCGGCCGCCTCGGCCCAGTCGACTCTAGACTCGAGCAA
GCTTATGCATGCGGCCGCAATTCGAGCTCACTTGGCCAATTCGCCCTATA
GTGAGTCGTATTAACTGGCCGTCGTTTTACAGATCCTGGCATGACTAGTG
CTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAA
CAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTC
CAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTA
CTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGG
CATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGC
FULL SEQUENCE: Available here
VECTOR MAP: not available
SEQUENCING PRIMERS
||

pENTR/D-TOPO

NAME: pENTR/D-TOPO (kanamycin resistant; 25 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: Gateway entry vector
POLYLINKER SEQUENCE:
TAACGCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC
AGTCTTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGAC
CTGTTCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTT
TGTACAAAAAAGCAGGCTCCGCGGCCGCCCCCTTCACC-ORF-AAGGGTG
GGCGCGCCGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCAT
TGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAAAAT
CATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACATGG
TCATAGCTGTTTCCTGGCAGCTCTG
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7
||

pENTR201

NAME: pENTR201 (kanamycin resistant; 10ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: Gateway entry vector
POLYLINKER SEQUENCE:
ACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACC
GCTAGCCAGGAAGAGTTTGTAGAAACGCAAAAAGGCCATCCGTCAGGATG
GCCTTCTGCTTAGTTTGATGCCTGGCAGTTTATGGCGGGCGTCCTGCCCG
CCACCCTCCGGGCCGTTGCTTCACAACGTTCAAATCCGCTCCCGGCGGAT
TTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAG
GCCCAGTCTTCCGACTGAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCC
CTACTCTCGCGTTAACGCTAGCATGGATCTCGGGCCCCAAATAATGATTT
TATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTGATGAGCAATGC
TTTTTTATAATGCCAACTTTGTACAAAAAAGCTGGCACCATG-linker-
ORF-linker-GGCGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGA
AAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAA
TAAAATCATTATTTGCCATCCAGCTGCAGCTCTGGCCCGTGTCTCAAAAT
CTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAA
ACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTC
AACGGGAAACGTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTA
TATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAAT
CTATCGCTTGTATGGGAAGCCCGATGCGC
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: pDONR201-forward [TCGCGTTAACGCTAGCATGGATCTC], pDONR201-reverse [GTAACATCAGAGATTTTGAGACAC]
||

pENTR221

NAME: pENTR221 (kanamycin resistant; 10ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: Gateway entry vector
POLYLINKER SEQUENCE:
CGACAAACAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTC
GTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATG
GATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCG
GGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAA
CAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGC
AGGCT-linker-ORF-linker-ACCCAGCTTTCTTGTACAAAGTTGGC
ATTATAAGAAAGCATTGCTTATCAATTTGTTGCAACGAACAGGTCACTAT
CAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCCCCTATAGTG
AGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCT
CAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAAC
AATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCC
ATATTCAACGGGAAACGTCGAGGCCGCGATTAAATTCCA
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse
||

pENTR223

NAME: pENTR223 (spectinomycin resistant; 100 ug/ml)
SOURCE: Dana Farber Cancer Institute/Vidal Lab
V_TYPE: Gateway entry vector
POLYLINKER SEQUENCE:
CAACAGATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT
TTGATGCCTGGCAGTTCCCTACTCTCGCGTTAACGCTAGCATGGATGTTT
TCCCAGTCACGACGTTGTAAAACGACGGCCAGTCTTAAGCTCGGGCCCCA
AATAATGATTTTATTTTGACTGATAGTGACCTGTTCGTTGCAACAAATTG
ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGTTG-link
er-ORF-linker-CCAACTTTCTTGTACAAAGTTGGCATTATAAGAAAG
CATTGCTTATCAATTTGTTGCAACGAACAGGTCACTATCAGTCAAAATAA
AATCATTATTTGCCATCCAGCTGATATCCCCTATAGTGAGTCGTATTACA
TGGTCATAGCTGTTTCCTGGCAGCTCTGGCCCGTGTCTCAAAATCTCTGA
TGTTACATTGCACAAGATAAAAATATATCATCATGCCTCCTCTAGACCAG
CCAGGACAGAAATGCCTCGACTTCGCTGC
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), t7
||

pENTR223.1

NAME: pENTR223.1 (spectinomycin resistant; 100 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: Gateway entry vector
POLYLINKER SEQUENCE:
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCAGAAG-linker-ORF-linker-GCCCAGCTTTCTTGTAC
AAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAACA
GGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATATCC
CCTATAGTGAGTCGTATTACATGGTCAT
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), t7
||

pENTR223.1-Sfi

NAME: pENTR223.1-Sfi (spectinomycin resistant; 100 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
GAGCCTTTCGTTTTATTTGATGCCTGGCAGTTCCCTACTCTCGCGTTAAC
GCTAGCATGGATGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTC
TTAAGCTCGGGCCCCAAATAATGATTTTATTTTGACTGATAGTGACCTGT
TCGTTGCAACAAATTGATGAGCAATGCTTTTTTATAATGCCAACTTTGTA
CAAAAAAGCAGAAGGGCCGTCAAGGCCCTGCAGACTGGCTGTGTATAAGG
GAGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGAT
GTCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGA
CCGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCG
ATATGCACCACCGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACG
TGCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATAT
CACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTG
TAAACCTTAAACTGCATTTCACCAGTCCCTGTTCTCGTCAGCAAAAGAGC
CGTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCC
GCTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGT
GCTTACCAGACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATA
GCTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCACACCTCT
TTTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAA
AATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGAT
CCACGCGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTC
ATTAAGCATTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCT
GAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCC
ATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATC
AAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCT
CAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACA
TCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACT
CCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAG
GGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGG
AATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGG
ATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATAT
CCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCC
TCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCC
AGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATA
ACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTG
GAACCTCTTACGTGCCGATCAACGTCTCATTTTCGCCAAAAGTTGGCCCA
GGGCTTCCCGGTATCAACAGGGACAGGCCTCATGGGCCCAGCTTTCTTGT
ACAAAGTTGGCATTATAAAAAATAATTGCTCATCAATTTGTTGCAACGAA
CAGGTCACTATCAGTCAAAATAAAATCATTATTTGCCATCCAGCTGATAT
CCCCTATAGTGAGTCGTATTACATGGTCATAGCTGTTTCCTGGCAGCTCT
GGCCCGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATA
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13F, T7 Rev
||

pET-28a

NAME: pET-28a (kanamycin resistant; 10 ug/ml)
SOURCE: Novagen
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: not available
VECTOR MAP: Available here
SEQUENCING PRIMERS: pET Upstream Primer, T7 Terminator Primer
||

pET-28b

NAME: pET-28b (kanamycin resistant; 10 ug/ml)
SOURCE: Novagen
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: not available
VECTOR MAP: Available here
SEQUENCING PRIMERS: pET Upstream Primer, T7 Terminator Primer
||

pExpress-1

NAME: pExpress-1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Express Genomics/Gruber
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gcagttttcccagtcacgacgttgtaaaacgacggccagtgcctagctta
taatacgactcactatagggaccactcctcgaggttaattaagcggccgc
cc...cDNA insert...atccgggaattccggaccgaaacttgttcta
tagtgtcacctaaataggcctaatggtcatagctgtttcctgtgtgaaat
tgttatccgct
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pFLC1

NAME: pFLC1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: RIKEN/Carninci
V_TYPE: phagemid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: not available
VECTOR MAP: not available
SEQUENCING PRIMERS: not available
||

pGA1

NAME: pGA1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pGA4

NAME: pGA4 (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pGA7

NAME: pGA7 (kanamycin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 reverse, M13 -20
||

pGA14

NAME: pGA14 (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pGA15

NAME: pGA15 (kanamycin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 reverse, M13 -20
||

pGA18

NAME: pGA18 (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pGA19

NAME: pGA19 (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pGA24

NAME: pGA24 (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pGH

NAME: pGH (ampicillin resistant; 50-200 ug/ml)
SOURCE: GeneArt
V_TYPE: phagemid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pME18S-FL3

NAME: pME18S-FL3 (ampicillin resistant; 50-200 ug/ml)
SOURCE: University of Tokyo/Sugano lab
V_TYPE: phagemid
POLYLINKER SEQUENCE:
tgtacggaagtgttacttctgctctaaaagctgcggaattcctcgagcac
tgtgtgatatccattgtgctggcgcggattctttatcactgataagttgg
tggacatattatgtttatcagtgataaagtgtcaagcatgacaaagttgc
agccgaatacagtgatccgtgccgccctagacctgttgaacgaggtcggc
gtagacggtctgacgacacgcaaactggcggaacggttgggggttcagca
gccggcgctttactggcacttcaggaacaagcgggcgctgctcgacgcac
tggccgaagccatgctggcggagaatcatagcacttcggtgccgagagcc
gacgacgactggcgctcatttctgactgggaatgcccgcagcttcaggca
ggcgctgctcgcctaccgccagcacaatggcaccatgtgctcgagctgca
ggtcgcggccgctaga
FULL SEQUENCE: Available here
VECTOR MAP: not available
SEQUENCING PRIMERS: custom
||

pOTB7

NAME: pOTB7 (chloramphenicol resistant; 25 ug/ml)
SOURCE: UCBerkeley/Rubin
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcgaattcccttactagtttccctcgaggcatctatgt
cgggtgcggagaaagaggtaatgaaatggcacatggtcatagctgtttcc
tgacccagctttcttgtacaaagtggtagatctgccggtctccctatagt
gagtcgtattaatttcgataagccaggttaacctgcattaatgaatcggc
tgcagtacccgggaatttaacccgcctaatgagcgggctttt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pOTB7-3

NAME: pOTB7-3 (chloramphenicol resistant; 10ug/ml)
SOURCE: University of Michigan, Ann Arbor
V_TYPE: phagemid
POLYLINKER SEQUENCE:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcgaattcccttactagtttccctcgagcggccgccag
tgtgctggaaagcatgttctttcctgcgttatcccctgattctgtggata
accgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacg
accgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacg
caaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtg
agttagctcactcattaggcaccccaggctttacactttatgcttccggc
tcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaaca
gctatgaccatgattacgccaagctatttaggtgacactatagaatactc
aagctatgcatcaagcttggtaccgagctcggatccactagtaacggccg
ccagtgtgctggaattctgcagatatccatcacactggcggccgctcgag
gcatctatgtcgggtgcggagaaagaggtaatgaaatggcacatggtcat
agctgtttcctgacccagctttcttgtacaaagt
FULL SEQUENCE: Available here
VECTOR MAP: not available
SEQUENCING PRIMERS: M13(-21), t7, sp6
||

pOTB7a

NAME: pOTB7a (chloramphenicol resistant; 25 ug/ml)
SOURCE: Edge Biosystems
V_TYPE: plasmid
POLYLINKER SEQUENCE:
cgttagaacgcggctacaattaatacataaccttatgtatcatacacata
cgatttaggtgacactatagaactcgaaggatccacaagtttgtacaaaa
aagcaggcttgtaaaacgacggccagtaactataacggtcctaaggtagc
gaggcctgggtggcctcgagccttccgaattctttcattacctctttctc
cgcaccccacataaagtgcggagaaagaggtaatgaaatggcacccagct
ttcttgtacaagtggtagatctgccggtctccctatagtgagtcgtatta
atttcgataagccaggttaacctgcattaatgaatcggctgcagtacccg
ggaatttaacccgcctaatgagc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), t7, sp6
||

pPCR-Script Amp SK(+)

NAME: pPCR-Script Amp SK(+) (ampicillin resistant; 50-200 ug/ml)
SOURCE: Stratagene
V_TYPE: plasmid
POLYLINKER SEQUENCE:
ttgtaaaacgacggccagtgagcgcgcgtaatacgactcactatagggcg
aattgggtaccgggccccccctcgaggtcgacggtatcgataagcttgat
atcgaattcctgcagcccgggggatccgcccgggctagagcggccgccac
cgcggtggagctccagcttttgttccctttagtgagggttaattgcgcgc
ttggcgtaatcatggtcatagctgtttcc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pRKW2

NAME: pRKW2 (ampicillin resistant; 50-200 ug/ml)
SOURCE: DKFZ/Niehrs
V_TYPE: phagemid
POLYLINKER SEQUENCE:
catacgatttaggtgacactatagaataacatccactttgcctttctctc
cacaggtgtccactcccaggtccaactgcacctcggttctatccgggccc
gaatacaagctacttgttctttttgcactcgag...5'-insert-3'..
.ggatccactagttctagagcggccgccaccgcggtggagcttggccgtg
gcccaacttgtttattgcagcttataatggttacaaataaagcaatagca
tcacaaatttcacaaataaag
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, AG195 [(forward): 5'-CATACGATTTAGGTGACACTATAG-3'], AG189 [(rev) 5'-CTTTATTTGTGAAATTTGTGATGC-3']
||

pSMART_cDNA

NAME: pSMART_cDNA (kanamycin resistant; 50-200 ug/ml)
SOURCE: Lucigen
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gaattcggcgcgcctaaacacataatcagccgagtctttgcaatagtaga
aattcatcactttctagatcctgaaccctatggacaccttcatcttcctc
cactcgtaatctccttgatactagattctctctatagcaggattcttcgc
acaaccagattctcagaaaaaccggtcagagaaaagctaaaccaaacaag
tcaaagcacaggaacgaacacatccaagaaacaagtgagaagcaacagtt
gttcagcaaaggtttggtgcggctacaatatttcgagctgattacagata
agaccaccgagcttatgtgcaacagggtgccctattgtctaaataactaa
tactccccgagtctgaaccgctcgatcgaacctcactcaacactgacgag
agcgagtcgtctaactcttgcagcctctttgcgtcgtgaaagctctctgg
atcctctagagtcgacctgcaggcatgcaagctt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: pSMART-1777F - GATCCTTTGATTTTCTACCGAAG, pSMART-200R - CTTACGTGCCGATCAAGTCA
||

pSPORT1

NAME: pSPORT1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
gaagagctatgacgtcgcatgcacgcgtacgtaagcttggatcctctaga
gcggccgc{3' - cDNA insert - 5'}gtcgacccgggaattccgg
accggtacctgcaggcgtaccagctt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pSPORT2

NAME: pSPORT2 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: phagemid
POLYLINKER SEQUENCE:
cattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggc
ctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgat
taagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacg
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagtcgacgagctcactagtga
cagcggccgccccgggaattccggaccggtacctgcaggcgtaccagctt
tccctatagtgagtcgtattagagcttggcgtaatcatggtcatagctgt
ttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagcc
ggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcac
attaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pT7T3D-PacI

NAME: pT7T3D-PacI (ampicillin resistant; 50-200 ug/ml)
SOURCE: Columbia University/Soares
V_TYPE: plasmid
POLYLINKER SEQUENCE:
tttaatacgactcactatagggaatttggccctcgaggccaagaattccc
gactacgtagtcggggatccgtcttaattaagcggccgcaagcttattcc
ctttagtgagggttaattttagcttggcactggccgtcgttttacaacgt
cgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcaca
tccccctttcgccagctggcgtaatagcgaagag
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, t3
||

pUC19

NAME: pUC19 (ampicillin resistant; 50-200 ug/ml)
SOURCE: New England Biolabs
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse
||

pUC19-Cam

NAME: pUC19-Cam (chloramphenicol resistant; 25 ug/ml)
SOURCE: Takara Bio Inc.
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse
||

pUC19-Kan

NAME: pUC19-Kan (kanamycin resistant; 50-200 ug/ml)
SOURCE: Takara Bio Inc.
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaat
gcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaac
gcaattaatgtgagttagctcactcattaggcaccccaggctttacactt
tatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttc
acacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcag
gtcgactctagaggatccccgggtaccgagctcgaattcactggccgtcg
ttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse
||

pUC19-Sfi

NAME: pUC19-Sfi (ampicillin resistant; 50-200 ug/ml)
SOURCE: Codon Devices Inc.
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gaattcggccacattggccatctcactggcacagctccacctggcccggc
ctcttttggacggccttcgaggccggatcctctagagtcgacctgcaggc
atgcaagctt
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse
||

pUC57

NAME: pUC57 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Fermentas
V_TYPE: plasmid
POLYLINKER SEQUENCE: not available
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13 -20, M13 reverse
||

pYX-Asc

NAME: pYX-Asc (ampicillin resistant; 50-200 ug/ml)
SOURCE: Columbia University/Soares
V_TYPE: plasmid
POLYLINKER SEQUENCE:
attaattaaccctcactaaagggacctaactataacggtcctaaggtagc
gagcggccgcggccagggaggccgatatcgaattcggcgcgccgtcgact
ttcattacctctttctccgcaccccacataaatctccctatagtgagtcg
tattaacatg
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: t7, t3, custom
||

pZERO-2

NAME: pZERO-2 (kanamycin resistant; 25 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
ggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaa
acagctatgaccatgattacgccaagctatttaggtgacactatagaata
ctcaagctatgcatcaagcttggtaccgagctcggatccactagtaacgg
ccgccagtgtgctggaattctgcagatatccatcacactggcggccgctc
gagcatgcatctagagggcccaattcgccctatagtgagtcgtattacaa
ttcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgtta
cccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaat
agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctata
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

pZL-1

NAME: pZL-1 (ampicillin resistant; 50-200 ug/ml)
SOURCE: Invitrogen Life Technologies
V_TYPE: plasmid
POLYLINKER SEQUENCE:
gccagtgaattgaatttaggtgacactatagaagagctatgacgtcgcat
gcacgcgtacgtaagcttggatcctctagagcggccgccgactagtgagc
tcgtcgacccgggaattccggaccggtacctgcaggcgtaccagctttcc
FULL SEQUENCE: Available here
VECTOR MAP: Available here
SEQUENCING PRIMERS: M13(-21), M13 reverse, t7, sp6
||

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