The I.M.A.G.E. Consortium

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Primate cDNA Libraries

Library information (by tissue)

brain/cns

1. NCI_CGAP_Brn72

ovary

2. NICHD_Rh_Ov1

testis

3. NICHD_Rh_Test1
4. NICHD_Rh_Test2

NCI_CGAP_Brn72

NAME: NCI_CGAP_Brn72
LIB_ID: 1807
ORGANISM: rhesus monkey
ORGAN: brain/CNS
TISSUE: hypothalamus
HOST: DH10B
VECTOR: pCMV-SPORT6.ccdb
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:

Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.2 kb. Constructed by Invitrogen (Carlsbad, CA).

NICHD_Rh_Ov1

NAME: NICHD_Rh_Ov1
LIB_ID: 1999
ORGANISM: rhesus monkey
STAGE: adult
ORGAN: ovary
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:

Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.0-4.0 kb. Tissue pooled from pre-pubertal, post pubertal and menopausal monkeys. Constructed by Clontech Laboratories (Palo Alto, CA).

NICHD_Rh_Test1

NAME: NICHD_Rh_Test1
LIB_ID: 2016
ORGANISM: rhesus monkey
SEX: male
STAGE: adult
ORGAN: testis
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:

5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT (30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.67 kb (range 0.8-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

NICHD_Rh_Test2

NAME: NICHD_Rh_Test2
LIB_ID: 2017
ORGANISM: rhesus monkey
SEX: male
STAGE: juvenile
ORGAN: testis
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:

5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.67 kb (range 0.8-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).

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