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Mouse cDNA Libraries


Vector information

Primer sequences

Library information (by tissue)

b-cell

    1.  NCI_CGAP_BC3
    2.  Soares NMGB
    3.  Soares NMGBC

blastocyst

    4.  NIA Mouse Blastocyst cDNA Library (Long)

blood

    5.  NCI_CGAP_BC1
    6.  NCI_CGAP_BC2
    7.  NCI_CGAP_MBM2

bone

    8.  NIA Mouse Osteoblast cDNA Library (Long 1)
    9.  NIA Mouse Osteoblast cDNA Library (Long)
    10.  Soares NMBP

bone marrow

    11.  Perkins LRH

bowel

    12.  Barstead MPL-RB9

brain/cns

    13.  Barstead MPL-RB12
    14.  Life Tech mouse brain (see comment)
    15.  Mione mouse WTB
    16.  NCI_CGAP_Brn63
    17.  NICHD_MM_Hyp1
    18.  NIH_BMAP_EF0
    19.  NIH_BMAP_EG0
    20.  NIH_BMAP_EG0p
    21.  NIH_BMAP_EH0
    22.  NIH_BMAP_EH0p
    23.  NIH_BMAP_EM0
    24.  NIH_BMAP_EQ0
    25.  NIH_BMAP_ER0
    26.  NIH_BMAP_EV0
    27.  NIH_BMAP_EW0
    28.  NIH_BMAP_EX0
    29.  NIH_BMAP_EY0
    30.  NIH_BMAP_FA0
    31.  NIH_BMAP_FB0
    32.  NIH_BMAP_FC0
    33.  NIH_BMAP_FD0
    34.  NIH_BMAP_FI0
    35.  NIH_BMAP_FO0
    36.  NIH_BMAP_FP0
    37.  NIH_BMAP_FR0
    38.  NIH_BMAP_FV0
    39.  NIH_BMAP_FW0
    40.  NIH_BMAP_FX0
    41.  NIH_BMAP_FY0
    42.  NIH_BMAP_GH0
    43.  NIH_BMAP_GI0
    44.  NIH_BMAP_GK0
    45.  NIH_BMAP_GL0
    46.  NIH_BMAP_GM0
    47.  NIH_BMAP_GV0
    48.  NIH_MGC_143
    49.  NIH_MGC_144
    50.  NIH_MGC_377
    51.  NIH_MGC_378
    52.  NIH_MGC_379
    53.  NIH_MGC_380
    54.  Soares NMAP
    55.  Soares NMBP1
    56.  Soares NMBP13-15
    57.  Soares NMBP2
    58.  Soares NMBPA
    59.  Soares NMHy

cartilage

    60.  Yamada 3-W rib cartilage

colon

    61.  Barstead MPL-RB6
    62.  Barstead MPL-RB7
    63.  NCI_CGAP_Co24
    64.  Schiller MAC13
    65.  Schiller MAC16

diaphragm

    66.  Stratagene mouse diaphragm (937303)

egg

    67.  Knowles/Solter egg
    68.  NIA Mouse Unfertilized Egg cDNA Library (Long 1)
    69.  NIA Mouse Unfertilized Egg cDNA Library (Long)

embryo

    70.  Baker mouse embryo e6.5
    71.  Baker mouse embryo e7.5
    72.  Beddington embryonic region
    73.  Knowles/Solter 11.5 dpc limb bud
    74.  Knowles/Solter 2-cell
    75.  Knowles/Solter 7.5 dpc primitive streak
    76.  Knowles/Solter 8-cell
    77.  Knowles/Solter E6.5dpc embryo
    78.  Knowles/Solter blastocyst
    79.  Knowles/Solter inner cell mass
    80.  Ko embryo 11.5dpc
    81.  Life Tech 10.5 dpc embryo (10665-016)
    82.  Life Tech 13.5 dpc embryo (10666-014)
    83.  Life Tech 15.5 dpc embryo (10667-012)
    84.  Life Tech 8.5 dpc embryo (10664-019)
    85.  NCI_CGAP_Emb3
    86.  NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long)
    87.  NIA Mouse 8.5-dpc Whole Embryo cDNA Library (Long)
    88.  NIA Mouse E10.5 whole embryo cDNA library (Long)
    89.  NIA Mouse E11.5 whole embryo cDNA library (Long)
    90.  NIA Mouse E13.5 whole embryo cDNA library (Long)
    91.  NIA Mouse E6.5 Whole Embryo cDNA library (Long)
    92.  NIA Mouse E9.5 Whole Embryo cDNA Library (Long)
    93.  NIA Mouse eight-cell-Embryo cDNA library (Long)
    94.  NIA Mouse four-cell-Embryo cDNA library (Long)
    95.  NIH_MGC_134
    96.  NIH_MGC_136
    97.  NIH_MGC_164
    98.  NIH_MGC_409
    99.  NIH_MGC_410
    100.  NIH_MGC_411
    101.  NIH_MGC_412
    102.  Soares 3NME12.5
    103.  Soares NMEBA
    104.  Soares NbME13.5-14.5
    105.  Soares p3NMF19.5
    106.  Stratagene embry. carcinoma/RA (937318)
    107.  Stratagene embryonic carcinoma (937317)
    108.  Sugano mouse embryo mewa
    109.  Yamada E13.5 limb bud
    110.  Yamada E13.5 tooth germ
    111.  Yamada E8.5 uni-craniofacial

embryonic stem cell

    112.  Knowles/Solter ES cell
    113.  NIA Mouse ES Cell (LIF-) cDNA Library (Long)
    114.  NIA Mouse Embryonic Stem (ES) cell (Lif+, 48 h, high density) cDNA library (Long)
    115.  NIA Mouse Embryonic Stem (ES) cell (Lif-, 48 h, high density) cDNA library (Long)
    116.  NIA Mouse Embryonic Stem (ES) cell (Lif-, 48 h, low density) cDNA library (Long)
    117.  NIA Mouse Undifferentiated ES Cell cDNA Library (Long)
    118.  NIA Mouse Undifferentiated Embryonic Stem (ES) Cell cDNA Library (Long 1)
    119.  Soares NMES

eye

    120.  NIH_BMAP_FZ0
    121.  NIH_BMAP_GW0
    122.  NIH_BMAP_GZ0
    123.  NIH_BMAP_HA0
    124.  NIH_BMAP_HB0
    125.  NIH_BMAP_HC0
    126.  NIH_BMAP_HD0
    127.  NIH_BMAP_HE0
    128.  NIH_BMAP_HP0
    129.  NIH_BMAP_HU0
    130.  NIH_BMAP_HV0
    131.  NIH_BMAP_HW0
    132.  NIH_BMAP_HX0
    133.  NIH_BMAP_HY0
    134.  NIH_BMAP_HY0p
    135.  NIH_BMAP_HZ0
    136.  NIH_BMAP_IB0
    137.  NIH_MGC_94
    138.  Rashbass MOC-10.5
    139.  Rashbass MOC-11.5
    140.  Rashbass MOV-9.5

genital ridge

    141.  NIA Mouse 12.5-dpc Male Genital Ridge/Mesonephros cDNA Library (Long)

germ cell

    142.  NIA Mouse Embryonic Germ Cell cDNA Library (Long)
    143.  NIA Mouse Embryonic Germ Cell cDNA Library (Long, subtracted)

gonad

    144.  Soares NMUR

head/neck

    145.  NCI_CGAP_SG1
    146.  NIH_BMAP_HJ0
    147.  NIH_BMAP_HK0
    148.  NIH_BMAP_HL0
    149.  NIH_BMAP_HN0
    150.  NIH_BMAP_HO0
    151.  NIH_BMAP_HQ0
    152.  NIH_BMAP_HS0

heart

    153.  Barstead MPL-RB3
    154.  NCI_CGAP_Ht1
    155.  Soares NbMH
    156.  Stratagene mouse heart (937316)

inner ear

    157.  NIH_MGC_130
    158.  Organ of Corti
    159.  Soares NMIE

kidney

    160.  Barstead MPL-RB1
    161.  Beier day 0 kidney
    162.  Beier day 7 kidney
    163.  NCI_CGAP_Ki15
    164.  NCI_CGAP_Kid14
    165.  NIA Mouse Newborn Kidney cDNA Library (Long 1)
    166.  NIA Mouse Newborn Kidney cDNA Library (Long)
    167.  NIH_MGC_154
    168.  NIH_MGC_176
    169.  Stratagene mouse kidney (937315)
    170.  Sugano mouse kidney mkia

limb

    171.  NIH_MGC_135

liver

    172.  NCI_CGAP_Li10
    173.  NCI_CGAP_Li9
    174.  NIH_MGC_152
    175.  NIH_MGC_177
    176.  Soares NML
    177.  Sugano mouse liver mlia

lung

    178.  Barstead MPL-RB2
    179.  NCI_CGAP_Lu29
    180.  NCI_CGAP_Lu30
    181.  NCI_CGAP_Lu33
    182.  NCI_CGAP_Lu35
    183.  NIH_MGC_155
    184.  NIH_MGC_413
    185.  NIH_MGC_414
    186.  NIH_MGC_415
    187.  NIH_MGC_416
    188.  Stratagene mouse lung (937302)

lymph node

    189.  Soares NbMLN

macrophage

    190.  Stratagene mouse macrophage (937306)

mammary gland

    191.  NCI_CGAP_Mam1
    192.  NCI_CGAP_Mam10
    193.  NCI_CGAP_Mam2
    194.  NCI_CGAP_Mam3
    195.  NCI_CGAP_Mam4
    196.  NCI_CGAP_Mam5
    197.  NCI_CGAP_Mam6
    198.  Soares NMLMG
    199.  Soares NbMMG

mandible

    200.  Soares NKWMD

maxillary process

    201.  Soares NMMAX

melanoma

    202.  Stratagene mouse melanoma (937312)

mixed

    203.  Barstead MPL-RB4
    204.  NIH_MGC_178
    205.  NIH_MGC_284
    206.  NIH_MGC_285
    207.  NIH_MGC_389
    208.  NIH_MGC_390
    209.  NIH_MGC_391
    210.  NIH_MGC_392
    211.  NIH_MGC_393
    212.  NIH_MGC_394
    213.  NIH_MGC_395
    214.  NIH_MGC_396

mouth

    215.  NCI_CGAP_SG2

muscle

    216.  Barstead MPL-RB13
    217.  Barstead MPL-RB14
    218.  Barstead MPL-RB5
    219.  Barstead MPL-RB8

nasal process

    220.  Soares NMKWNP

olfactory epithelium

    221.  NIH_MGC_129
    222.  NIH_MGC_399
    223.  NIH_MGC_400

oocyte

    224.  Eppig/Hampl oocyte
    225.  NIH_MGC_256
    226.  NIH_MGC_257

ovary

    227.  NCI_CGAP_Ov44
    228.  Soares NMO1
    229.  Soares NMO2

pancreas

    230.  Amplified Melton mouse islets 1 MIS1-A
    231.  Kaestner ngn3 -/-
    232.  Kaestner ngn3 wt
    233.  Melton amplified mouse E10.5/12.5 pancreas 1
    234.  Melton amplified mouse E16.5 pancreas3 M16S1-A
    235.  Melton mouse E10.5/12.5 pancreas
    236.  Melton mouse E16.5 pancreas M16Z1
    237.  Melton mouse E16.5 pancreas library 2 M16B2
    238.  Melton mouse adult pancreas 1 MAZ1
    239.  Melton mouse adult pancreas 2 MAZ2
    240.  Melton mouse islets MIZ1
    241.  Melton mouse newborn pancreas MNZ1
    242.  Melton normalized mixed mouse pancreas 1 N1-MMS1
    243.  NIH_MGC_137
    244.  NIH_MGC_138
    245.  NIH_MGC_139
    246.  NIH_MGC_140

pituitary gland

    247.  NIH_MGC_166
    248.  Schiller AtT-20
    249.  Schiller AtT-20, RESP18 induced

placenta

    250.  NIH_MGC_203
    251.  NIH_MGC_204
    252.  NIH_MGC_222
    253.  NIH_MGC_223
    254.  Soares 4NbMP13.5-14.5

prostate

    255.  Soares MPR0
    256.  Soares NMPR0

skin

    257.  NCI_CGAP_Skn2
    258.  Stratagene mouse skin

small intestine

    259.  NCI_CGAP_SI1

spleen

    260.  Barstead MPL-RB10
    261.  NCI_CGAP_Sp2
    262.  Soares 3NbMS

stem cell

    263.  NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1+) cDNA Library (Long 1)
    264.  NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1+) cDNA Library (Long)
    265.  NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit+/Sca-1-) cDNA Library (Long)
    266.  NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1+) cDNA Library (Long 1)
    267.  NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1+) cDNA Library (Long)
    268.  NIA Mouse Hematopoietic Stem Cell (Lin-/c-Kit-/Sca-1-) cDNA Library (Long)
    269.  NIA Mouse Mesenchymal Stem Cell cDNA Library (Long 1)
    270.  NIA Mouse Mesenchymal Stem Cell cDNA Library (Long)
    271.  NIA Mouse Neural Stem Cell (Differentiated) cDNA Library (Long)
    272.  NIA Mouse Neural Stem Cell (Undifferentiated) cDNA Library (Long)
    273.  NIA Mouse Trophoblast Stem Cell cDNA Library (Long 1)
    274.  NIA Mouse Trophoblast Stem Cell cDNA Library (Long)
    275.  NIH_MGC_149
    276.  NIH_MGC_150
    277.  NIH_MGC_196

stomach

    278.  NCI_CGAP_St1

synthesized dna

    279.  NIH_MGC_426
    280.  NIH_MGC_427
    281.  NIH_MGC_482
    282.  NIH_MGC_483
    283.  NIH_MGC_484
    284.  NIH_MGC_488

t-cell

    285.  Stratagene mouse T-cell (937311)

testis

    286.  Barstead MPL-RB11
    287.  Barstead MPL-RB15
    288.  Barstead MPL-RB16
    289.  McCarrey/Eddy 18-20 day sertoli cell
    290.  McCarrey/Eddy 18-day leptotene and zygotene spermatocytes
    291.  McCarrey/Eddy 18-day preleptotene spermatocytes
    292.  McCarrey/Eddy 6-day primitive type A spermatogonia
    293.  McCarrey/Eddy adult testis
    294.  McCarrey/Eddy round spermatid
    295.  McCarrey/Eddy spermatocytes
    296.  McCarrey/Eddy type A spermatogonia
    297.  McCarrey/Eddy type B spermatogonia
    298.  NCI_CGAP_Te1
    299.  NIH_MGC_165
    300.  NIH_MGC_169
    301.  NIH_MGC_381
    302.  NIH_MGC_382
    303.  NIH_MGC_383
    304.  NIH_MGC_384
    305.  Stratagene mouse testes

thymus

    306.  NIH_MGC_385
    307.  NIH_MGC_386
    308.  NIH_MGC_387
    309.  NIH_MGC_388
    310.  Ren/Stubbs mouse thymus
    311.  Soares 2NbMT

thyroid

    312.  NIH_MGC_189
    313.  NIH_MGC_230

tooth

    314.  NIH_MGC_190
    315.  Yamada E19.5 molar

trophoblast

    316.  Soares NMTC

uterus

    317.  Soares NMPu


NCI_CGAP_BC3

NAME: NCI_CGAP_BC3
LIB_ID: 1648
ORGANISM: mouse
ORGAN: B-cell
TISSUE: flow-sorted lymphocytes, marginal zone B-cell tumor
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
mRNA made from flow-sorted lymphocytes, cDNA made by oligo-dT priming. Directionally cloned. Average insert size 1.8 kb. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Note: this is a NCI_CGAP Library.
||

Soares NMGB

NAME: Soares NMGB
LIB_ID: 1382
ORGANISM: mouse
ORGAN: B-cell
TISSUE: germinal B-cell
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTGGTTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized; constructed by Bento Soares and M.Fatima Bonaldo.
||

Soares NMGBC

NAME: Soares NMGBC
LIB_ID: 1497
ORGANISM: mouse
ORGAN: B-cell
TISSUE: germinal B cell from resting spleen
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGGATTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo.
||

NIA Mouse Blastocyst cDNA Library (Long)

NAME: NIA Mouse Blastocyst cDNA Library (Long)
LIB_ID: 1983
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: mixed
ORGAN: Blastocyst
TISSUE: pool of 20 blastocysts
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was extracted from a pool of 20 blastocysts. Double-stranded cDNAs were synthesized with an oligo-dT primer (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') from 0.2 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC- 3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pSPORT1 plasmid vector. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

NCI_CGAP_BC1

NAME: NCI_CGAP_BC1
LIB_ID: 1601
ORGANISM: mouse
ORGAN: blood
TISSUE: flow-sorted, bone marrow progenitors
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from bone marrow progenitors, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_BC2

NAME: NCI_CGAP_BC2
LIB_ID: 1602
ORGANISM: mouse
ORGAN: blood
TISSUE: flow-sorted, bone marrow progenitors
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from bone marrow progenitors, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_MBM2

NAME: NCI_CGAP_MBM2
LIB_ID: 1687
ORGANISM: mouse
ORGAN: blood
TISSUE: flow-sorted bone marrow progenitors
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from flow-sorted, bone marrow progenitors, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIA Mouse Osteoblast cDNA Library (Long 1)

NAME: NIA Mouse Osteoblast cDNA Library (Long 1)
LIB_ID: 2099
ORGANISM: mouse
STRAIN: C3H/He
SEX: unknown
ORGAN: bone
TISSUE: Osteoblast, stage KUSA/A1 cells
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 2.1 5g of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0 kb. The library was constructed by Yulan Piao.
||

NIA Mouse Osteoblast cDNA Library (Long)

NAME: NIA Mouse Osteoblast cDNA Library (Long)
LIB_ID: 1994
ORGANISM: mouse
STRAIN: C3H/He
SEX: unknown
ORGAN: bone
TISSUE: Osteoblast, stage KUSA/A1 cells
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was obtained from Dr. Akihiro Umezawa (Keio University School of Medicine, Japan). Double- stranded cDNAs were synthesized with an oligo-dT primer (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3')from 2.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC-3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pSPORT1 plasmid vector. The average insert size is about 3 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

Soares NMBP

NAME: Soares NMBP
LIB_ID: 1441
ORGANISM: mouse
ORGAN: bone
TISSUE: bone pool
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGCCGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo (University of Iowa).
||

Perkins LRH

NAME: Perkins LRH
LIB_ID: 1472
ORGANISM: mouse
STRAIN: BALB/c
SEX: female
STAGE: adult
ORGAN: bone marrow
TISSUE: primary sorted bone marrow cells
HOST: GeneHogs DH10B
VECTOR: pZL-1
V_TYPE: plasmid
RE_5': EagI
RE_3': SalI
DESCR:
cDNA made by oligo-dT priming. Library amplified by stretch PCR. Subtraction method: Bonaldo, et al., Genome Research 6:791. Library constructed by Dr. Archibald Perkins (Yale University).
||

Barstead MPL-RB9

NAME: Barstead MPL-RB9
LIB_ID: 564
ORGANISM: mouse
STRAIN: FVB/N
STAGE: adult
ORGAN: bowel
TISSUE: small bowel harvested 72 hours after irradiation with 1400 Gys
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [AATTCGTCGACATC], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation). Source irradiated bowel harvested 72 hours after irradiation (1400 Gys).
||

Barstead MPL-RB12

NAME: Barstead MPL-RB12
LIB_ID: 1064
ORGANISM: mouse
STRAIN: C57BL/6
SEX: male
STAGE: adult
ORGAN: brain/CNS
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [5' AATTCGGATCCTTC 3' and 5' GAAGGATCCG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

Life Tech mouse brain (see comment)

NAME: Life Tech mouse brain (see comment)
LIB_ID: 220
ORGANISM: mouse
STAGE: adult
ORGAN: brain/CNS
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. pCMV-SPORT2 vector. *COMMENT: Sequence analysis indicates this library is likely to be of rat origin.
||

Mione mouse WTB

NAME: Mione mouse WTB
LIB_ID: 1437
ORGANISM: mouse
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled forebrains
HOST: DH12S
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Size-selected 1.5 kb for average insert size 2 kb. Primary library; non-amplified. This library was constructed by M. Mione (University College London, Dept of Anatomy and Developmental Biology).
||

NCI_CGAP_Brn63

NAME: NCI_CGAP_Brn63
LIB_ID: 1633
ORGANISM: mouse
SEX: female
STAGE: juvenile
ORGAN: brain/CNS
TISSUE: brain
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NICHD_MM_Hyp1

NAME: NICHD_MM_Hyp1
LIB_ID: 2034
ORGANISM: mouse
STRAIN: C57BL
SEX: both
STAGE: mixed
ORGAN: brain/CNS
TISSUE: normal mediobasal and hypothalamus
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5' -ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.08 kb (range 0.73-1.37 kb). 13/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
||

NIH_BMAP_EF0

NAME: NIH_BMAP_EF0
LIB_ID: 1880
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc (size selected for the 0.5-1 kb fragments), containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EG0

NAME: NIH_BMAP_EG0
LIB_ID: 1881
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc (size selected for the 2-3 kb fragments), containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EG0p

NAME: NIH_BMAP_EG0p
LIB_ID: 1882
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc (size selected for the 2-3 kb fragments), containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EH0

NAME: NIH_BMAP_EH0
LIB_ID: 1883
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc, containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EH0p

NAME: NIH_BMAP_EH0p
LIB_ID: 1884
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc (size selected for the 3-4 kb fragments), containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EM0

NAME: NIH_BMAP_EM0
LIB_ID: 1885
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc (size selected for the 1-2 kb fragments), containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EQ0

NAME: NIH_BMAP_EQ0
LIB_ID: 1894
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: GeneHogs DH10B
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 18.5pc (size selected for the 4-5 kb fragments), containing 210,000 recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_ER0

NAME: NIH_BMAP_ER0
LIB_ID: 1896
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: brain, pooled
HOST: GeneHogs DH10B
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 15.5pc (size selected for the 3-4 kb fragments), containing 3 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EV0

NAME: NIH_BMAP_EV0
LIB_ID: 1910
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 15.5pc (size selected for the 2-3 kb fragments), containing 3.5 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EW0

NAME: NIH_BMAP_EW0
LIB_ID: 1911
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 15.5pc (size selected for the 3-4 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EX0

NAME: NIH_BMAP_EX0
LIB_ID: 1925
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 15.5pc (size selected for the 4-5 kb fragments. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_EY0

NAME: NIH_BMAP_EY0
LIB_ID: 1926
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 15.5pc (size selected for the 5-7 kb fragments. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FA0

NAME: NIH_BMAP_FA0
LIB_ID: 1967
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 12.5pc (size selected for the 0.5-1 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FB0

NAME: NIH_BMAP_FB0
LIB_ID: 1968
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 12.5pc (size selected for the 1-2 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. (Contains the same tissue as NIH_BMAP_FA0). Tissue was obtained from the Jim Lin lab at the University of Iowa,library construction by Maria de Fatima Bonaldo and M. Bento Soares(University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FC0

NAME: NIH_BMAP_FC0
LIB_ID: 1969
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 12.5pc (size selected for the 3-4 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. (Contains the same tissue as NIH_BMAP_FA0). Tissue was obtained from the Jim Lin lab at the University of Iowa,library construction by Maria de Fatima Bonaldo and M. Bento Soares(University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FD0

NAME: NIH_BMAP_FD0
LIB_ID: 1970
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 12.5pc (size selected for the 2-3 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. (Contains the same tissue as NIH_BMAP_FA0). Tissue was obtained from the Jim Lin lab at the University of Iowa,library construction by Maria de Fatima Bonaldo and M. Bento Soares(University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project..
||

NIH_BMAP_FI0

NAME: NIH_BMAP_FI0
LIB_ID: 1971
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 12.5pc (size selected for the 4-5 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. (Contains the same tissue as NIH_BMAP_FA0). Tissue was obtained from the Jim Lin lab at the University of Iowa,library construction by Maria de Fatima Bonaldo and M. Bento Soares(University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FO0

NAME: NIH_BMAP_FO0
LIB_ID: 1972
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 12.5pc (size selected for the 5-7 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. (Contains the same tissue as NIH_BMAP_FA0). Tissue was obtained from the Jim Lin lab at the University of Iowa,library construction by Maria de Fatima Bonaldo and M. Bento Soares(University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FP0

NAME: NIH_BMAP_FP0
LIB_ID: 1973
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue day 15.5pc (size selected for the 0.5-1 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCACGACTTTTTTTTTTTTTTTTTT -3') contains the sequence tag CAGCCACGAC between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. (Contains existing tissue from LIB_ID 956). Tissue was obtained from the Jim Lin lab at the University of Iowa,library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FR0

NAME: NIH_BMAP_FR0
LIB_ID: 1974
ORGANISM: mouse
STRAIN: C57BL/6
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue embryonic day, 13.5, 14.5, 16.5 and 17.5 (size selected for the 2-3 kb fragments), containing 2.2 million recombinants. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCAGCCAC GACTTTTTTTTTTTTTTTTTT -3')contains the sequence tag CAGCCACGAC between the NotI cloning siteand dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FV0

NAME: NIH_BMAP_FV0
LIB_ID: 2019
ORGANISM: mouse
STRAIN: C57BL/6
SEX: unknown
ORGAN: brain/CNS
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue embryonic day, 13.5, 14.5, 16.5 and 17.5 (size selected for the 0.5-1 kb fragments). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCAGCGAGACAGTTTTTTTTTTTTTTTTTT-3')contains the sequence tag AGCGAGACAG between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FW0

NAME: NIH_BMAP_FW0
LIB_ID: 2020
ORGANISM: mouse
STRAIN: C57BL/6
SEX: unknown
ORGAN: brain/CNS
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue embryonic day, 13.5, 14.5, 16.5 and 17.5 (size selected for the 3-4 kb fragments). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCAGCGAGACAGTTTTTTTTTTTTTTTTTT-3')contains the sequence tag AGCGAGACAG between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FX0

NAME: NIH_BMAP_FX0
LIB_ID: 2021
ORGANISM: mouse
STRAIN: C57BL/6
SEX: unknown
ORGAN: brain/CNS
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue embryonic day, 13.5, 14.5, 16.5 and 17.5 (size selected for the 1-2 kb fragments). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCAGCGAGACAGTTTTTTTTTTTTTTTTTT-3')contains the sequence tag AGCGAGACAG between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_FY0

NAME: NIH_BMAP_FY0
LIB_ID: 2022
ORGANISM: mouse
STRAIN: C57BL/6
SEX: unknown
ORGAN: brain/CNS
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue embryonic day, 13.5, 14.5, 16.5 and 17.5 (size selected for the 4-5 kb fragments). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCAGCGAGACAGTTTTTTTTTTTTTTTTTT-3')contains the sequence tag AGCGAGACAG between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_GH0

NAME: NIH_BMAP_GH0
LIB_ID: 2036
ORGANISM: mouse
STRAIN: C57BL/6
STAGE: adult
ORGAN: brain/CNS
TISSUE: whole brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue, adult days 1, 5 and 15 (size selected). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTTT-3')contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Size-selected for the 4-5 kb fraction. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy Project (BMAP).
||

NIH_BMAP_GI0

NAME: NIH_BMAP_GI0
LIB_ID: 2035
ORGANISM: mouse
STRAIN: C57BL/6
SEX: unknown
ORGAN: brain/CNS
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue embryonic day, 13.5, 14.5, 16.5 and 17.5 (size selected for the 5-7 kb fraction). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCAGCGAGACAGTTTTTTTTTTTTTTTTTT-3')contains the sequence tag AGCGAGACAG between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Tissue was obtained from the Jim Lin lab at the University of Iowa, library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_GK0

NAME: NIH_BMAP_GK0
LIB_ID: 2037
ORGANISM: mouse
STRAIN: C57BL/6
STAGE: adult
ORGAN: brain/CNS
TISSUE: whole brain, pooled
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue, adult days 1, 5 and 15 (size selected). The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTTT-3')contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy Project (BMAP).
||

NIH_BMAP_GL0

NAME: NIH_BMAP_GL0
LIB_ID: 2070
ORGANISM: mouse
STRAIN: C57BL/6
STAGE: juvenile
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue days 1, 5 and 15. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first- synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTT-3') contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_GM0

NAME: NIH_BMAP_GM0
LIB_ID: 2071
ORGANISM: mouse
STRAIN: C57BL/6
STAGE: juvenile
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue days 1, 5 and 15. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first- synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTT-3') contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_BMAP_GV0

NAME: NIH_BMAP_GV0
LIB_ID: 2073
ORGANISM: mouse
STRAIN: C57BL/6
STAGE: juvenile
ORGAN: brain/CNS
TISSUE: pooled brain
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled mouse brain tissue days 1, 5 and 15. The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first- synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCCGAACTGAATTTTTTTTTTTTTTTTTT-3') contains the sequence tag CGAACTGAAT between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library construction by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa) for the NIH Brain Mouse Anatomy (BMAP) Project.
||

NIH_MGC_143

NAME: NIH_MGC_143
LIB_ID: 1961
ORGANISM: mouse
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: brain
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' cDNA made by oligo-dT priming and directionally cloned. 5'' and 3'' adaptors were used in cloning as follows: 5''-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'' and 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3''. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.2-0.5 kb size fraction (other fractions present in NIH_MGC_144). Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library. '
||

NIH_MGC_144

NAME: NIH_MGC_144
LIB_ID: 1962
ORGANISM: mouse
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: brain
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' cDNA made by oligo-dT priming and directionally cloned. 5'' and 3'' adaptors were used in cloning as follows: 5''-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'' and 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3''. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.2-0.5 kb size fraction (other fractions present in NIH_MGC_143). Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library. '
||

NIH_MGC_377

NAME: NIH_MGC_377
LIB_ID: 2392
ORGANISM: mouse
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_378 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_378

NAME: NIH_MGC_378
LIB_ID: 2393
ORGANISM: mouse
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_377 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_379

NAME: NIH_MGC_379
LIB_ID: 2394
ORGANISM: mouse
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_380 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_380

NAME: NIH_MGC_380
LIB_ID: 2395
ORGANISM: mouse
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_379 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

Soares NMAP

NAME: Soares NMAP
LIB_ID: 1720
ORGANISM: mouse
STAGE: adult
ORGAN: brain/CNS
TISSUE: pituitary gland
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCACGCGTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
||

Soares NMBP1

NAME: Soares NMBP1
LIB_ID: 1683
ORGANISM: mouse
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pituitary gland
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a NotI- oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGAATAATACATTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacII vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
||

Soares NMBP13-15

NAME: Soares NMBP13-15
LIB_ID: 1737
ORGANISM: mouse
STAGE: juvenile
ORGAN: brain/CNS
TISSUE: pituitary
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
1st strand cDNA was primed with a NotI- oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCTGTACCGATGTTTTTTTTTTTTTTTTTTT-3'; double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
||

Soares NMBP2

NAME: Soares NMBP2
LIB_ID: 1682
ORGANISM: mouse
STAGE: embryo
ORGAN: brain/CNS
TISSUE: pituitary gland
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a NotI- oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGGCGCTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacII vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
||

Soares NMBPA

NAME: Soares NMBPA
LIB_ID: 1738
ORGANISM: mouse
STAGE: adult
ORGAN: brain/CNS
TISSUE: pituitary
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
1st strand cDNA was primed with a NotI - oligo(dT) primer 5'-AACTGGAAGAATTCGCGGCCGCGTATCCATGATTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' AND 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
||

Soares NMHy

NAME: Soares NMHy
LIB_ID: 865
ORGANISM: mouse
ORGAN: brain/CNS
TISSUE: hypothalamus
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCAAGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. RNA provided by Dr. Wolfgang Liedtke. Library went through two rounds of normalization, and was constructed by Bento Soares and M.Fatima Bonaldo.
||

Yamada 3-W rib cartilage

NAME: Yamada 3-W rib cartilage
LIB_ID: 1322
ORGANISM: mouse
SEX: unknown
STAGE: juvenile
ORGAN: cartilage
TISSUE: rib cartilage
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
||

Barstead MPL-RB6

NAME: Barstead MPL-RB6
LIB_ID: 442
ORGANISM: mouse
STRAIN: FVB/N
STAGE: infant
ORGAN: colon
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [AATTCGGATCCTTC], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

Barstead MPL-RB7

NAME: Barstead MPL-RB7
LIB_ID: 443
ORGANISM: mouse
STRAIN: FVB/N
STAGE: adult
ORGAN: colon
TISSUE: harvested 72 hours after irradiation with 1400 Gys
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Tissue obtained from 8 week old mouse. Colon was harvested 72hours after irradiation with 1400 Gys. 1st strand cDNA was primed with a Not I - oligo(dT) primer [5'TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [AATTCGTCGACATG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

NCI_CGAP_Co24

NAME: NCI_CGAP_Co24
LIB_ID: 1674
ORGANISM: mouse
STRAIN: FVB/N
SEX: male
STAGE: adult
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

Schiller MAC13

NAME: Schiller MAC13
LIB_ID: 1283
ORGANISM: mouse
ORGAN: colon
TISSUE: colon cancer cell line MAC13
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from cell line MAC13 usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
||

Schiller MAC16

NAME: Schiller MAC16
LIB_ID: 1284
ORGANISM: mouse
ORGAN: colon
TISSUE: colon cancer cell line MAC16
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from cell line MAC16 usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
||

Stratagene mouse diaphragm (937303)

NAME: Stratagene mouse diaphragm (937303)
LIB_ID: 282
ORGANISM: mouse
STAGE: adult
ORGAN: diaphragm
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally from mRNA prepared from diaphragm muscle. Primer: Oligo dT. Average insert size: 1.5 kb. Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Knowles/Solter egg

NAME: Knowles/Solter egg
LIB_ID: 389
ORGANISM: mouse
STRAIN: B6D2F1/J
SEX: neither
STAGE: embryo
ORGAN: egg
TISSUE: 5000 pooled unfertilized eggs
HOST: DH10B
VECTOR: pBluescribe (modified)
V_TYPE: phagemid
RE_5': MluI
RE_3': SalI
DESCR:
Cloned unidirectionally from mRNA prepared from 5000 unfertilized eggs. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.0 kb.
||

NIA Mouse Unfertilized Egg cDNA Library (Long 1)

NAME: NIA Mouse Unfertilized Egg cDNA Library (Long 1)
LIB_ID: 2103
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
ORGAN: egg
TISSUE: 1488 pooled unfertilized eggs
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Mouse cDNA project by the Laboratory of Genetics, National Institute on Aging (NIA), Intramural Research Program, NIH (http://lgsun.grc.nia.nih.gov/cDNA). This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 1488 unfertilized eggs. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'], treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao.
||

NIA Mouse Unfertilized Egg cDNA Library (Long)

NAME: NIA Mouse Unfertilized Egg cDNA Library (Long)
LIB_ID: 1990
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
ORGAN: egg
TISSUE: 1488 pooled unfertilized eggs
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was extracted from a pool of 148 unfertilized eggs. Double-stranded cDNAs were synthesized with an oligo-dT primer (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'), treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC-3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pSPORT1 plasmid vector. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

Baker mouse embryo e6.5

NAME: Baker mouse embryo e6.5
LIB_ID: 1817
ORGANISM: mouse
STRAIN: CD-1
STAGE: embryo
ORGAN: embryo
TISSUE: early gastrula
HOST: XL-1 Blue
VECTOR: pCS105
V_TYPE: plasmid
RE_5': SalI
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Directionally cloned into SalI/NotI sites using the following 5' adaptor: 5'-TCGACCCACGCGTCCG-3'. Size-selected for average insert size 1.8-1.9 kb. Library constructed by J. Baker (Stanford University).
||

Baker mouse embryo e7.5

NAME: Baker mouse embryo e7.5
LIB_ID: 1818
ORGANISM: mouse
STRAIN: CD-1
STAGE: embryo
ORGAN: embryo
TISSUE: late gastrula
HOST: XL-1 Blue
VECTOR: pCS105
V_TYPE: plasmid
RE_5': SalI
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Directionally cloned into SalI/NotI sites using the following 5' adaptor: 5'-TCGACCCACGCGTCCG-3'. Size-selected for average insert size 1.8-1.9 kb. Library constructed by J. Baker (Stanford University).
||

Beddington embryonic region

NAME: Beddington embryonic region
LIB_ID: 299
ORGANISM: mouse
STRAIN: C57BL/6J x DBA
STAGE: fetal
ORGAN: embryo
TISSUE: pooled gastrulating embryos (excluding those with head folds/extraembryonic tissues)
HOST: DH12S
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Gastrulating embryos were collected at 7.5dpc from C57BL6 x DBA matings, excluding embryos that had developed head folds and all extraembryonic tissues. Average insert size: 1.3 kb (range: 0.5 - 3.0 kb) Reference: Development 121, 2479-2489 (1995).
||

Knowles/Solter 11.5 dpc limb bud

NAME: Knowles/Solter 11.5 dpc limb bud
LIB_ID: 395
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
TISSUE: limb bud
HOST: DH10B
VECTOR: pBluescript SK+
V_TYPE: phagemid
RE_5': XbaI
RE_3': XhoI
DESCR:
Cloned unidirectionally from mRNA prepared from limb bud tissue at 11.5 days post-conception. Primer: Oligo dT. cDNAs were cloned into the XbaI/XhoI sites of pBluescript SK+ (Stratagene) using commercial linkers (NEB). Average insert size: 1.0 kb.
||

Knowles/Solter 2-cell

NAME: Knowles/Solter 2-cell
LIB_ID: 390
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
TISSUE: 13500 pooled 2-cell stage embryos
HOST: DH10B
VECTOR: pBluescribe (modified)
V_TYPE: phagemid
RE_5': MluI
RE_3': SalI
DESCR:
Cloned unidirectionally from mRNA prepared from 13,500 2-cell stage embryos. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB). Average insert size: 1.2 kb.
||

Knowles/Solter 7.5 dpc primitive streak

NAME: Knowles/Solter 7.5 dpc primitive streak
LIB_ID: 394
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
TISSUE: primitive streak
HOST: DH10B
VECTOR: pBluescript SK+
V_TYPE: phagemid
RE_5': XbaI
RE_3': XhoI
DESCR:
Cloned unidirectionally from mRNA prepared from primitive streak embryonic tissue. Primer: Oligo dT. cDNAs were cloned into the XbaI/XhoI sites of pBluescript SK+ (Stratagene) using commercial linkers (NEB). Average insert size: 1.0 kb.
||

Knowles/Solter 8-cell

NAME: Knowles/Solter 8-cell
LIB_ID: 391
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
TISSUE: 2000 pooled 8-cell stage
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally from mRNA prepared from 2,000 8-cell stage embryos. Primer: NotI(dT): 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Average insert size: 0.7 kb.
||

Knowles/Solter E6.5dpc embryo

NAME: Knowles/Solter E6.5dpc embryo
LIB_ID: 451
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
HOST: DH10B
VECTOR: pBluescribe (modified)
V_TYPE: phagemid
RE_5': MluI
RE_3': SalI
DESCR:
Cloned unidirectionally from mRNA prepared from 6.5 dpc embryo. Primer: SalI(dT): 5'-CGGTCGACCGTCGACCGTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the MluI/SalI sites of a modified pBluescribe vector using commercial linkers (NEB).
||

Knowles/Solter blastocyst

NAME: Knowles/Solter blastocyst
LIB_ID: 392
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
TISSUE: 800 pooled blastocysts
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally from mRNA prepared from 800 blastocysts. Primer: NotI(dT): 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNAs were cloned into the NotI/SalI sites of a pSPORT vector (Life Technologies). Two different size selections: B1 (larger inserts) and B3. Average insert size of combined library is 1 kb.
||

Knowles/Solter inner cell mass

NAME: Knowles/Solter inner cell mass
LIB_ID: 396
ORGANISM: mouse
STRAIN: B6D2F1/J
STAGE: embryo
ORGAN: embryo
TISSUE: inner cell mass
HOST: DH10B
VECTOR: pBluescript SK+
V_TYPE: phagemid
RE_5': XbaI
RE_3': XhoI
DESCR:
Cloned unidirectionally from mRNA prepared from inner cell mass embryonic tissue. Primer: Oligo dT. cDNAs were cloned into the XbaI/XhoI sites of pBluescript SK+ (Stratagene) using commercial linkers (NEB). Average insert size: 0.5 kb.
||

Ko embryo 11.5dpc

NAME: Ko embryo 11.5dpc
LIB_ID: 398
ORGANISM: mouse
STRAIN: C57BL/6J
STAGE: embryo
ORGAN: embryo
TISSUE: pooled whole embryos, excluding placenta and yolk sac
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Total RNAs were extracted from 11.5 dpc embryos (excluding placenta and yolk sac). The double-stranded cDNA was synthesized with an oligo (dT)-1 primer GAGAGAGACTAGTTCTAGATCGCGAGCGGCCGCTTTTTTTTTTTTTTTTTT 3'. The cDNAs were ligated to LL-Sal3A: 5' GCTATTGACGTCGACTATCC 3' and LL-Sal3B: 5' GGATAGTCGACGTCAAT 3'. The cDNAs were size-selected and amplified by long-range PCR using Ex Taq polymerase for 18 cycles. The PCR-amplifiable cDNA mixture went through one round of equalization and was digested with SalI/NotI and cloned into the SalI/NotI sites of the pSPORT1 plasmid vector (Life Technologies). The library was constructed by Dr. Minoru S. H. Ko and Dr. Xiaohong Wang.
||

Life Tech 10.5 dpc embryo (10665-016)

NAME: Life Tech 10.5 dpc embryo (10665-016)
LIB_ID: 301
ORGANISM: mouse
STRAIN: C57BL/6J x DBA
STAGE: embryo
ORGAN: embryo
TISSUE: pooled embryos
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. 10.5dpc embryos. pCMV-SPORT2 vector.
||

Life Tech 13.5 dpc embryo (10666-014)

NAME: Life Tech 13.5 dpc embryo (10666-014)
LIB_ID: 302
ORGANISM: mouse
STRAIN: C57BL/6J x DBA
STAGE: embryo
ORGAN: embryo
TISSUE: pooled embryos
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. 13.5dpc embryos. pCMV-SPORT2 vector.
||

Life Tech 15.5 dpc embryo (10667-012)

NAME: Life Tech 15.5 dpc embryo (10667-012)
LIB_ID: 303
ORGANISM: mouse
STRAIN: C57BL/6J x DBA
STAGE: embryo
ORGAN: embryo
TISSUE: pooled embryos
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. 15.5dpc embryos. pCMV-SPORT2 vector.
||

Life Tech 8.5 dpc embryo (10664-019)

NAME: Life Tech 8.5 dpc embryo (10664-019)
LIB_ID: 300
ORGANISM: mouse
STRAIN: C57BL/6J
STAGE: embryo
ORGAN: embryo
TISSUE: pooled embryos
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. 8.5dpc embryos. pCMV-SPORT2 vector.
||

NCI_CGAP_Emb3

NAME: NCI_CGAP_Emb3
LIB_ID: 1600
ORGANISM: mouse
STAGE: embryo
ORGAN: embryo
TISSUE: primitive streak
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from primitive streak, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long)

NAME: NIA Mouse 7.5-dpc Whole Embryo cDNA Library (Long)
LIB_ID: 1982
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: Whole embryo including extra embryonic tissues
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was extracted from a pool of four embryos at 7.5 days postcoitum. Double-stranded cDNAs were synthesized with an oligo-dT primer (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') from 7 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC-3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pSPORT1 plasmid vector. The average insert size is about 2.2 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

NIA Mouse 8.5-dpc Whole Embryo cDNA Library (Long)

NAME: NIA Mouse 8.5-dpc Whole Embryo cDNA Library (Long)
LIB_ID: 1985
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: Pool of 13 embryos including extra embryonic tissues
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was extracted from a pool of 13 embryos at 8.5 days postcoitum. Double-stranded cDNAs were synthesized with an oligo-dT primer (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') from 9.1 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC-3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pSPORT1 plasmid vector. The average insert size is about 2.5 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

NIA Mouse E10.5 whole embryo cDNA library (Long)

NAME: NIA Mouse E10.5 whole embryo cDNA library (Long)
LIB_ID: 2141
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: whole embryo including extraembryonic tissues, pool of 8
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 8 embryos at 10.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 25g of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.4Kb. The library was constructed by Yulan Piao.
||

NIA Mouse E11.5 whole embryo cDNA library (Long)

NAME: NIA Mouse E11.5 whole embryo cDNA library (Long)
LIB_ID: 2142
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: whole embryo including extraembryonic tissues, pool of 3
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from a pool of 3 embryos at 11.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 25g of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.3Kb. The library was constructed by Yulan Piao.
||

NIA Mouse E13.5 whole embryo cDNA library (Long)

NAME: NIA Mouse E13.5 whole embryo cDNA library (Long)
LIB_ID: 2137
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: whole embryo (one) including extraembryonic tissues
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Total RNAs were extracted from 1 embryo at 13.5-days postcoitum. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 35g of total RNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.0Kb. The library was constructed by Yulan Piao.
||

NIA Mouse E6.5 Whole Embryo cDNA library (Long)

NAME: NIA Mouse E6.5 Whole Embryo cDNA library (Long)
LIB_ID: 2047
ORGANISM: mouse
STRAIN: C57BL/6J
STAGE: embryo
ORGAN: embryo
TISSUE: whole embryo including extraembryonic tissues
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was obtained from a pool of seven embryos at 6.5 days post-conception. Double-stranded cDNAs were synthesized with an oligo-dT primer (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3')from 0.53 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC-3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pCMV-SPORT6 plasmid vector. The average insert size is about 2.3 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

NIA Mouse E9.5 Whole Embryo cDNA Library (Long)

NAME: NIA Mouse E9.5 Whole Embryo cDNA Library (Long)
LIB_ID: 1989
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: whole embryo including extraembryonic tissues
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library. Total RNA was extracted from a pool of 16 embryos at 9.5 days postcoitum. Double-stranded cDNAs were synthesized with an oligo-dT primer (5'- pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3') from 6 ug of total RNA, treated with T4 DNA polymerase, and purified by ethanol precipitation. The cDNAs were ligated to Lone-linker LL-Sal4 (5'-AAGAGGTAATCC-3'), purified by phenol/chloroform, and separated from free linker by Centricon 100. The cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with primer Sal4-S. The cDNAs were digested with SalI and Not1I enzymes and cloned into SalI/NotI sites of pSPORT1 plasmid vector. The average insert size is about 3 kb. The library was constructed by Yulan Piao and Minoru Ko (NIH, NIA). Reference: Genome Res (2001) 11:1553-1558 [PMID: 11544199].
||

NIA Mouse eight-cell-Embryo cDNA library (Long)

NAME: NIA Mouse eight-cell-Embryo cDNA library (Long)
LIB_ID: 2143
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: 8-cell embryo, pool of 360
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). The mRNAs were extracted from a pool of 360 embryos at 8-cell stage. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 18ng of mRNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.7Kb. The library was constructed by Yulan Piao.
||

NIA Mouse four-cell-Embryo cDNA library (Long)

NAME: NIA Mouse four-cell-Embryo cDNA library (Long)
LIB_ID: 2144
ORGANISM: mouse
STRAIN: C57BL/6J
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: 4-cell embryos, pool of 360
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). The mRNAs were extracted from a pool of 360 embryos at 4-cell stage. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3'] from 10.8ng of mRNA, treated with T4 DNA polymerase, and purified by ethanol-precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform, and separated from free linkers by Centricon 100. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform and Centricon 100. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV-SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 2.2Kb. The library was constructed by Yulan Piao.
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NIH_MGC_134

NAME: NIH_MGC_134
LIB_ID: 1963
ORGANISM: mouse
STAGE: embryo
ORGAN: embryo
TISSUE: pool of undifferentiated limb containing undifferentiated limb mesenchyme and early condensing mesenchyme.
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library. '
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NIH_MGC_136

NAME: NIH_MGC_136
LIB_ID: 2003
ORGANISM: mouse
STRAIN: CD-1
SEX: unknown
STAGE: embryo
ORGAN: embryo
TISSUE: limb, maxilla and mandible: 5, 4 and 1 limb and jaw equivalents from 17.5, 18.5 and newborn days respectively
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
'Normalized, full-length enriched library from pool of mouse embronic limb, maxilla and mandible, embryonic day 17.5, 18.5 and newborn (mandible (5, 4 and 1 limb and jaw equivalents from respective days). Cloned directionally, oligo-dT primed (5''-GACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3''. Size selected for the 1kb fragments, average insert size 1.2 kb. Normalization to Cot 7.5 . Tissue contributed by David Rowe; library constructed by ResGen, Invitrogen Corp. Note: this is a NIH_MGC Library. '
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NIH_MGC_164

NAME: NIH_MGC_164
LIB_ID: 1997
ORGAN