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Human cDNA Libraries


Vector information

Primer sequences

Library information (by tissue)

adipose

    1.  NCI_CGAP_Lip2

adrenal gland

    2.  NCI_CGAP_AA1
    3.  NCI_CGAP_Adr1
    4.  NCI_CGAP_Phe1
    5.  NIH_MGC_84

b-cell

    6.  NCI_CGAP_CLL1
    7.  NCI_CGAP_GCB0
    8.  NCI_CGAP_GCB1
    9.  NIH_MGC_48

bladder

    10.  NIH_MGC_53
    11.  NIH_MGC_93

blastocyst

    12.  NIH_MGC_278
    13.  NIH_MGC_279
    14.  NIH_MGC_280
    15.  NIH_MGC_281

blood

    16.  NCI_CGAP_CML1
    17.  NIH_MGC_106
    18.  NIH_MGC_2
    19.  Soares NPBMC

bone

    20.  NCI_CGAP_DF0
    21.  NCI_CGAP_DF1
    22.  NCI_CGAP_ED0
    23.  NCI_CGAP_ED1
    24.  NCI_CGAP_EI0
    25.  NCI_CGAP_EI1
    26.  NCI_CGAP_Ew1
    27.  NCI_CGAP_FG0
    28.  NCI_CGAP_FG1
    29.  NCI_CGAP_Fs1
    30.  NCI_CGAP_Pr12
    31.  NCI_CGAP_SS1
    32.  NIH_MGC_86

bone marrow

    33.  Jia bone marrow stroma
    34.  NCI_CGAP_HSC1
    35.  NCI_CGAP_HSC3
    36.  NCI_CGAP_HSC4
    37.  NIH_MGC_54
    38.  NIH_MGC_55

bowel

    39.  NCI_CGAP_Mel1
    40.  NCI_CGAP_Mel3

brain/cns

    41.  COGENE 4AR (4EAR)
    42.  COGENE PR (4EPR)
    43.  Clontech brain (HL3002s)
    44.  Clontech fetal brain (HL3025s)
    45.  Johnston frontal cortex
    46.  Lehrach HBF
    47.  Life Tech brain (10418-010)
    48.  NCI_CGAP_Brn20
    49.  NCI_CGAP_Brn21
    50.  NCI_CGAP_Brn23
    51.  NCI_CGAP_Brn25
    52.  NCI_CGAP_Brn35
    53.  NCI_CGAP_Brn41
    54.  NCI_CGAP_Brn50
    55.  NCI_CGAP_Brn52
    56.  NCI_CGAP_Brn53
    57.  NCI_CGAP_Brn64
    58.  NCI_CGAP_Brn65
    59.  NCI_CGAP_Brn66
    60.  NCI_CGAP_Brn67
    61.  NCI_CGAP_Brn70
    62.  NCI_CGAP_CNS1
    63.  NCI_CGAP_Pit1
    64.  NCI_CGAP_Sch1
    65.  NICHD_HS_Ut1
    66.  NICHD_HS_Ut2
    67.  NIH_MGC_114
    68.  NIH_MGC_119
    69.  NIH_MGC_121
    70.  NIH_MGC_124
    71.  NIH_MGC_126
    72.  NIH_MGC_127
    73.  NIH_MGC_128
    74.  NIH_MGC_141
    75.  NIH_MGC_142
    76.  NIH_MGC_181
    77.  NIH_MGC_19
    78.  NIH_MGC_192
    79.  NIH_MGC_227
    80.  NIH_MGC_228
    81.  NIH_MGC_229
    82.  NIH_MGC_264
    83.  NIH_MGC_266
    84.  NIH_MGC_272
    85.  NIH_MGC_274
    86.  NIH_MGC_286
    87.  NIH_MGC_287
    88.  NIH_MGC_288
    89.  NIH_MGC_289
    90.  NIH_MGC_306
    91.  NIH_MGC_307
    92.  NIH_MGC_308
    93.  NIH_MGC_309
    94.  NIH_MGC_310
    95.  NIH_MGC_311
    96.  NIH_MGC_312
    97.  NIH_MGC_313
    98.  NIH_MGC_47
    99.  NIH_MGC_56
    100.  NIH_MGC_57
    101.  NIH_MGC_73
    102.  NIH_MGC_95
    103.  NIH_MGC_96
    104.  NIH_MGC_98
    105.  Schiller astrocytoma
    106.  Schiller glioblastoma multiforme
    107.  Schiller meningioma
    108.  Schiller oligodendroglioma
    109.  Schneider fetal brain 00004
    110.  Soares 1NIB
    111.  Soares 2NbHMSP
    112.  Soares N2b4HB55Y
    113.  Soares N2b5HB55Y
    114.  Soares NbHFB
    115.  Stanley Frontal B/N pool 1
    116.  Stanley Frontal B/N pool 2
    117.  Stanley Frontal N/B pool 2
    118.  Stanley Frontal N/S pool 2
    119.  Stanley Frontal S/B pool 1
    120.  Stanley Frontal S/N individual
    121.  Stanley Frontal S/N pool 1
    122.  Stanley Frontal S/N pool 2
    123.  Stanley Hippocampus B/N pool 1
    124.  Stanley Hippocampus B/S pool 1
    125.  Stanley Hippocampus N/B pool 1
    126.  Stanley Hippocampus N/S pool 1
    127.  Stanley Hippocampus S/B pool 1
    128.  Stanley Hippocampus S/N pool 1
    129.  Stratagene NT2 neuroepithelium (937230)
    130.  Stratagene NT2/RA neuroepithelium 937231
    131.  Stratagene NT2/RA+MI neuroepith (937234)
    132.  Stratagene fetal brain (937226)
    133.  Stratagene mature hNT (937233)
    134.  Stratagene schizophrenic brain S11

breast

    135.  NCI_CGAP_Br1.1
    136.  NCI_CGAP_Br12
    137.  NCI_CGAP_Br13
    138.  NCI_CGAP_Br14
    139.  NCI_CGAP_Br15
    140.  NCI_CGAP_Br16
    141.  NCI_CGAP_Br17
    142.  NCI_CGAP_Br18
    143.  NCI_CGAP_Br2
    144.  NCI_CGAP_Br22
    145.  NCI_CGAP_Br3
    146.  NCI_CGAP_Br4
    147.  NCI_CGAP_Br5
    148.  NCI_CGAP_Br7
    149.  NIH_MGC_107
    150.  NIH_MGC_151
    151.  NIH_MGC_87
    152.  Soares 2NbHBst
    153.  Soares 3NbHBst

cartilage

    154.  NCI_CGAP_Car1
    155.  NCI_CGAP_EU0
    156.  NCI_CGAP_EU1
    157.  NCI_CGAP_EZ0
    158.  NCI_CGAP_EZ1
    159.  NCI_CGAP_FE0
    160.  NCI_CGAP_FE1
    161.  NCI_CGAP_FH0
    162.  NCI_CGAP_FH1
    163.  NCI_CGAP_FL0
    164.  NCI_CGAP_FL1

cervix

    165.  Clontech HeLa S3 cells (HL3021s)
    166.  NIH_MGC_12
    167.  NIH_MGC_13
    168.  NIH_MGC_35
    169.  NIH_MGC_4
    170.  NIH_MGC_5
    171.  NIH_MGC_64
    172.  Soares NHCe
    173.  Soares NHCeC
    174.  Stratagene HeLa S3 cells (937216)

cochlea

    175.  Morton fetal cochlea

colon

    176.  Barstead HPL-RB7
    177.  NCI_CGAP_Co1
    178.  NCI_CGAP_Co10
    179.  NCI_CGAP_Co11
    180.  NCI_CGAP_Co12
    181.  NCI_CGAP_Co14
    182.  NCI_CGAP_Co16
    183.  NCI_CGAP_Co17
    184.  NCI_CGAP_Co18
    185.  NCI_CGAP_Co19
    186.  NCI_CGAP_Co2
    187.  NCI_CGAP_Co20
    188.  NCI_CGAP_Co21
    189.  NCI_CGAP_Co22
    190.  NCI_CGAP_Co23
    191.  NCI_CGAP_Co25
    192.  NCI_CGAP_Co26
    193.  NCI_CGAP_Co27
    194.  NCI_CGAP_Co28
    195.  NCI_CGAP_Co29
    196.  NCI_CGAP_Co3
    197.  NCI_CGAP_Co4
    198.  NCI_CGAP_Co8
    199.  NCI_CGAP_Co9
    200.  NIH_MGC_15
    201.  NIH_MGC_268
    202.  NIH_MGC_276
    203.  NIH_MGC_294
    204.  NIH_MGC_295
    205.  NIH_MGC_296
    206.  NIH_MGC_297
    207.  NIH_MGC_65
    208.  Soares/Dieckgraefe NHCD
    209.  Soares/Dieckgraefe NHUC
    210.  Stratagene colon tumor (937204)
    211.  Stratagene colon tumor (937221)

connective tissue

    212.  NCI_CGAP_Sar4

embryonic stem cell

    213.  NIA Human H1 Embryonic Stem Cells cDNA Library (Long)
    214.  NIH_MGC_193
    215.  NIH_MGC_200
    216.  NIH_MGC_201
    217.  NIH_MGC_202
    218.  NIH_MGC_239
    219.  NIH_MGC_240
    220.  NIH_MGC_258
    221.  NIH_MGC_259
    222.  NIH_MGC_260
    223.  NIH_MGC_261
    224.  NIH_MGC_262
    225.  NIH_MGC_263

endothelial cell

    226.  Stratagene endothelial cell (937223)

esophagus

    227.  NCI_CGAP_Eso2

eye

    228.  NIH_MGC_16
    229.  NIH_MGC_43
    230.  NIH_MGC_67
    231.  Soares N2b4HR
    232.  Soares N2b5HR
    233.  Stratagene corneal stroma (937222)
    234.  Stratagene fetal retina (937202)

fetus

    235.  Soares Nb2HF8-9w

fibroblast

    236.  Soares NbHSF
    237.  Stratagene fibroblast (937212)

genitourinary

    238.  NCI_CGAP_GU1

germ cell

    239.  NCI_CGAP_GC1
    240.  NCI_CGAP_GC2
    241.  NCI_CGAP_GC3
    242.  NCI_CGAP_GC4
    243.  NCI_CGAP_GC5
    244.  NCI_CGAP_GC6

gingiva

    245.  NCI_CGAP_HN5
    246.  NCI_CGAP_HN6

gut

    247.  NIH_MGC_182

head/neck

    248.  COGENE 4PA1
    249.  COGENE 6E MAN
    250.  COGENE 6E MAX
    251.  COGENE 8.5 EAT
    252.  COGENE 8.5 EPT
    253.  NCI_CGAP_HN20
    254.  NCI_CGAP_HN21
    255.  NIH_MGC_102

heart

    256.  Barstead HPL-RB3
    257.  Barstead HPL-RB6
    258.  Clontech heart (HL3026s)
    259.  Life Tech heart (10419-018)
    260.  NIH_MGC_74
    261.  Soares NbHH19W

kidney

    262.  Clontech kidney (HL3001s)
    263.  Gessler Wilms' tumor
    264.  Life Tech kidney (10420-016)
    265.  NCI_CGAP_Kid1
    266.  NCI_CGAP_Kid11
    267.  NCI_CGAP_Kid12
    268.  NCI_CGAP_Kid13
    269.  NCI_CGAP_Kid3
    270.  NCI_CGAP_Kid5
    271.  NCI_CGAP_Kid6
    272.  NCI_CGAP_Kid7
    273.  NCI_CGAP_Kid8
    274.  NIH_MGC_108
    275.  NIH_MGC_14
    276.  NIH_MGC_45
    277.  NIH_MGC_58
    278.  NIH_MGC_75
    279.  NIH_MGC_89

larynx

    280.  NCI_CGAP_HN2
    281.  NCI_CGAP_Lar1

leukocyte

    282.  Life Tech leukocyte (10421-014)
    283.  NIH_MGC_118

liver

    284.  Clontech fetal liver (HL3020s)
    285.  Clontech liver (HL3006s)
    286.  Life Tech liver (10422-012)
    287.  NCI_CGAP_Gas2
    288.  NCI_CGAP_Li1
    289.  NCI_CGAP_Li2
    290.  NCI_CGAP_Li5
    291.  NCI_CGAP_Li8
    292.  NCI_CGAP_Pr20
    293.  NIH_MGC_100
    294.  NIH_MGC_290
    295.  NIH_MGC_291
    296.  NIH_MGC_292
    297.  NIH_MGC_293
    298.  NIH_MGC_76
    299.  NIH_MGC_90
    300.  Stratagene liver (937224)

lung

    301.  Clontech fetal lung (HL3022s)
    302.  Clontech lung (HL3004s)
    303.  Life Tech lung (10424-018)
    304.  NCI_CGAP_DH0
    305.  NCI_CGAP_DH1
    306.  NCI_CGAP_DI0
    307.  NCI_CGAP_DT0
    308.  NCI_CGAP_DT1
    309.  NCI_CGAP_Lu1
    310.  NCI_CGAP_Lu13
    311.  NCI_CGAP_Lu19
    312.  NCI_CGAP_Lu21
    313.  NCI_CGAP_Lu24
    314.  NCI_CGAP_Lu25
    315.  NCI_CGAP_Lu26
    316.  NCI_CGAP_Lu27
    317.  NCI_CGAP_Lu28
    318.  NCI_CGAP_Lu31
    319.  NCI_CGAP_Lu34
    320.  NCI_CGAP_Lu34.1
    321.  NCI_CGAP_Lu5
    322.  NCI_CGAP_Lu6
    323.  NCI_CGAP_Lu7
    324.  NIH_MGC_101
    325.  NIH_MGC_18
    326.  NIH_MGC_212
    327.  NIH_MGC_213
    328.  NIH_MGC_214
    329.  NIH_MGC_215
    330.  NIH_MGC_265
    331.  NIH_MGC_273
    332.  NIH_MGC_298
    333.  NIH_MGC_299
    334.  NIH_MGC_300
    335.  NIH_MGC_301
    336.  NIH_MGC_59
    337.  NIH_MGC_68
    338.  NIH_MGC_69
    339.  NIH_MGC_7
    340.  NIH_MGC_77
    341.  Soares NbHL19W
    342.  Stratagene lung (937210)
    343.  Stratagene lung carcinoma (937218)

lymph

    344.  NIH_MGC_3
    345.  NIH_MGC_36
    346.  NIH_MGC_37
    347.  NIH_MGC_38
    348.  NIH_MGC_50
    349.  NIH_MGC_51
    350.  NIH_MGC_52
    351.  NIH_MGC_63
    352.  NIH_MGC_8
    353.  NIH_MGC_85
    354.  NIH_MGC_99

lymph node

    355.  NCI_CGAP_HN1
    356.  NCI_CGAP_HSC2
    357.  NCI_CGAP_Lym12
    358.  NCI_CGAP_Lym3
    359.  NCI_CGAP_Lym5
    360.  NCI_CGAP_Lym6

macrophage

    361.  NCI_CGAP_FT0
    362.  NCI_CGAP_FT1
    363.  NCI_CGAP_FT2

mixed

    364.  DFCI-Vidal horfeome 1.1
    365.  MAPcL
    366.  NCI_CGAP_Sub1
    367.  NCI_CGAP_Sub2
    368.  NCI_CGAP_Sub3
    369.  NCI_CGAP_Sub4
    370.  NCI_CGAP_Sub5
    371.  NCI_CGAP_Sub6
    372.  NCI_CGAP_Sub7
    373.  NCI_CGAP_Sub8
    374.  NCI_CGAP_Sub9
    375.  NIH_MGC_115
    376.  NIH_MGC_116
    377.  NIH_MGC_117
    378.  NIH_MGC_120
    379.  NIH_MGC_122
    380.  NIH_MGC_145
    381.  NIH_MGC_146
    382.  NIH_MGC_184
    383.  NIH_MGC_186
    384.  NIH_MGC_187
    385.  NIH_MGC_195
    386.  NIH_MGC_217
    387.  NIH_MGC_218
    388.  NIH_MGC_219
    389.  NIH_MGC_220
    390.  NIH_MGC_221
    391.  NIH_MGC_241
    392.  NIH_MGC_244
    393.  NIH_MGC_245
    394.  NIH_MGC_271
    395.  NIH_MGC_277
    396.  NIH_MGC_282
    397.  NIH_MGC_283
    398.  NIH_MGC_314
    399.  NIH_MGC_315
    400.  NIH_MGC_316
    401.  NIH_MGC_317
    402.  NIH_MGC_318
    403.  NIH_MGC_319
    404.  NIH_MGC_320
    405.  NIH_MGC_321
    406.  NIH_MGC_322
    407.  NIH_MGC_323
    408.  NIH_MGC_324
    409.  NIH_MGC_325
    410.  NIH_MGC_326
    411.  NIH_MGC_327
    412.  NIH_MGC_328
    413.  NIH_MGC_329
    414.  NIH_MGC_330
    415.  NIH_MGC_332
    416.  NIH_MGC_333
    417.  NIH_MGC_334
    418.  NIH_MGC_335
    419.  NIH_MGC_336
    420.  NIH_MGC_337
    421.  NIH_MGC_338
    422.  NIH_MGC_339
    423.  NIH_MGC_340
    424.  NIH_MGC_341
    425.  NIH_MGC_342
    426.  NIH_MGC_343
    427.  NIH_MGC_344
    428.  NIH_MGC_345
    429.  NIH_MGC_346
    430.  NIH_MGC_347
    431.  NIH_MGC_348
    432.  NIH_MGC_349
    433.  NIH_MGC_350
    434.  NIH_MGC_351
    435.  NIH_MGC_352
    436.  NIH_MGC_353
    437.  NIH_MGC_354
    438.  NIH_MGC_355
    439.  NIH_MGC_356
    440.  NIH_MGC_357
    441.  NIH_MGC_358
    442.  NIH_MGC_359
    443.  NIH_MGC_360
    444.  NIH_MGC_361
    445.  NIH_MGC_362
    446.  NIH_MGC_363
    447.  NIH_MGC_364
    448.  NIH_MGC_397
    449.  NIH_MGC_398
    450.  Soares 1NFLS
    451.  Soares 1NFLS-S1
    452.  Soares NFL_T_GBC_S1
    453.  Soares NSF-F8-9W-OT-PA-P-S1
    454.  Soares NbHPU
    455.  Soares NhHMPu-S1

mouth

    456.  NCI_CGAP_HN10
    457.  NCI_CGAP_HN15
    458.  NCI_CGAP_HN16
    459.  NCI_CGAP_HN7
    460.  NCI_CGAP_HN8
    461.  NCI_CGAP_HN9

muscle

    462.  Barstead HPL-RB8
    463.  Clontech muscle (HL3000s)
    464.  NCI_CGAP_AR1
    465.  NCI_CGAP_Alv1
    466.  NIH_MGC_123
    467.  NIH_MGC_17
    468.  NIH_MGC_183
    469.  NIH_MGC_81
    470.  Stratagene skeletal muscle (937209)

olfactory epithelium

    471.  Weizmann olfactory epithelium

ovary

    472.  NCI_CGAP_Ov1
    473.  NCI_CGAP_Ov10
    474.  NCI_CGAP_Ov11
    475.  NCI_CGAP_Ov18
    476.  NCI_CGAP_Ov2
    477.  NCI_CGAP_Ov23
    478.  NCI_CGAP_Ov26
    479.  NCI_CGAP_Ov31
    480.  NCI_CGAP_Ov32
    481.  NCI_CGAP_Ov33
    482.  NCI_CGAP_Ov34
    483.  NCI_CGAP_Ov35
    484.  NCI_CGAP_Ov36
    485.  NCI_CGAP_Ov37
    486.  NCI_CGAP_Ov38
    487.  NCI_CGAP_Ov39
    488.  NCI_CGAP_Ov40
    489.  NCI_CGAP_Ov41
    490.  NCI_CGAP_Ov5
    491.  NCI_CGAP_Ov6
    492.  NCI_CGAP_Ov8
    493.  NICHD_Hs_Ov1
    494.  NIH_MGC_109
    495.  NIH_MGC_125
    496.  NIH_MGC_66
    497.  NIH_MGC_9
    498.  Soares NbHOT
    499.  Stratagene ovary (937217)
    500.  Stratagene ovary tumor (937219)

pancreas

    501.  Barstead HPL-RB1
    502.  HR85 islet
    503.  Human insulinoma
    504.  Melton human islets HIZ1
    505.  Melton human pancreatic islets
    506.  Melton normalized human islet 4 N4-HIS1
    507.  NCI_CGAP_PI1
    508.  NCI_CGAP_Pan1
    509.  NCI_CGAP_Pan3
    510.  NIH_MGC_110
    511.  NIH_MGC_39
    512.  NIH_MGC_42
    513.  NIH_MGC_70
    514.  NIH_MGC_78
    515.  Stratagene pancreas tumor (937208)
    516.  Takeda/Bell pancreatic islet

parathyroid

    517.  Soares NbHPA

peripheral nervous system

    518.  Human Anterior Horn
    519.  Lupski dorsal root ganglion
    520.  Lupski sciatic nerve
    521.  Lupski sympathetic trunk
    522.  NCI_CGAP_PNS1

pharynx

    523.  NCI_CGAP_HN17
    524.  NCI_CGAP_HN19
    525.  NCI_CGAP_HN4

pineal gland

    526.  Soares 1NbHPG
    527.  Soares 3NbHPG

pituitary gland

    528.  NIH_MGC_179

placenta

    529.  Gatewood hydatidiform mole
    530.  NIH_MGC_10
    531.  NIH_MGC_11
    532.  NIH_MGC_147
    533.  NIH_MGC_148
    534.  NIH_MGC_21
    535.  NIH_MGC_302
    536.  NIH_MGC_303
    537.  NIH_MGC_304
    538.  NIH_MGC_305
    539.  NIH_MGC_79
    540.  Soares 2NbHP8-9W
    541.  Soares Nb2HP
    542.  Stratagene placenta (937225)

prostate

    543.  Barstead HPL-RB4
    544.  Barstead HPL-RB4.1
    545.  NCI_CGAP_Pr1
    546.  NCI_CGAP_Pr10
    547.  NCI_CGAP_Pr11
    548.  NCI_CGAP_Pr15
    549.  NCI_CGAP_Pr16
    550.  NCI_CGAP_Pr18
    551.  NCI_CGAP_Pr2
    552.  NCI_CGAP_Pr21
    553.  NCI_CGAP_Pr22
    554.  NCI_CGAP_Pr23
    555.  NCI_CGAP_Pr24
    556.  NCI_CGAP_Pr25
    557.  NCI_CGAP_Pr28
    558.  NCI_CGAP_Pr3
    559.  NCI_CGAP_Pr4
    560.  NCI_CGAP_Pr4.1
    561.  NCI_CGAP_Pr5
    562.  NCI_CGAP_Pr6
    563.  NCI_CGAP_Pr7
    564.  NCI_CGAP_Pr8
    565.  NCI_CGAP_Pr9
    566.  NCI_CGAP_RDF1
    567.  NCI_CGAP_RDF2
    568.  NIH_MGC_111
    569.  NIH_MGC_210
    570.  NIH_MGC_40
    571.  NIH_MGC_60
    572.  NIH_MGC_83
    573.  NIH_MGC_91

skin

    574.  NCI_CGAP_Mel15
    575.  NCI_CGAP_Skn1
    576.  NCI_CGAP_Skn3
    577.  NCI_CGAP_Skn4
    578.  NIH_MGC_112
    579.  NIH_MGC_20
    580.  NIH_MGC_41
    581.  NIH_MGC_49
    582.  NIH_MGC_62
    583.  NIH_MGC_72
    584.  Soares 2NbHM

small intestine

    585.  NIH_MGC_88

soft tissue

    586.  NCI_CGAP_Lei2

spleen

    587.  Barstead HPL-RB2
    588.  Life Tech spleen (10425-015)
    589.  NIH_MGC_113
    590.  Stratagene fetal spleen (937205)

stomach

    591.  NCI_CGAP_Gas1
    592.  NCI_CGAP_Gas4
    593.  NCI_CGAP_St3

synthesized dna

    594.  NIH_MGC_242
    595.  NIH_MGC_243
    596.  NIH_MGC_369
    597.  NIH_MGC_370
    598.  NIH_MGC_371
    599.  NIH_MGC_372
    600.  NIH_MGC_425
    601.  NIH_MGC_428
    602.  NIH_MGC_479
    603.  NIH_MGC_480
    604.  NIH_MGC_481
    605.  NIH_MGC_486

t-cell

    606.  NIH_MGC_191

testis

    607.  Barstead HPL-RB5
    608.  Life Tech testis (10426-013)
    609.  NIH_MGC_180
    610.  NIH_MGC_267
    611.  NIH_MGC_275
    612.  NIH_MGC_373
    613.  NIH_MGC_374
    614.  NIH_MGC_375
    615.  NIH_MGC_376
    616.  NIH_MGC_61
    617.  NIH_MGC_82
    618.  NIH_MGC_92
    619.  NIH_MGC_97
    620.  Soares NHT

thymus

    621.  NCI_CGAP_Thym1
    622.  Soares NHFTh

thyroid

    623.  NCI_CGAP_Thy1
    624.  NCI_CGAP_Thy10
    625.  NCI_CGAP_Thy11
    626.  NCI_CGAP_Thy12
    627.  NCI_CGAP_Thy3
    628.  NCI_CGAP_Thy4
    629.  NCI_CGAP_Thy5
    630.  NCI_CGAP_Thy6
    631.  NCI_CGAP_Thy7
    632.  NCI_CGAP_Thy8

tongue

    633.  NCI_CGAP_HN11
    634.  NCI_CGAP_HN12
    635.  NCI_CGAP_HN13
    636.  NCI_CGAP_HN14
    637.  NCI_CGAP_HN3

trophoblast

    638.  NIH_MGC_194

unspecified

    639.  DFCI-Kazusa.orf
    640.  DFCI-RIKEN.orf
    641.  DKFZo001
    642.  DKFZo002
    643.  DKFZo003
    644.  DKFZo004
    645.  DKFZo005
    646.  DKFZo006
    647.  DKFZo007
    648.  DKFZo008
    649.  DKFZo009
    650.  DKFZo010
    651.  Kazusa-KIAA-pBCSK+
    652.  Kazusa-KIAA-pBluescriptIISK+
    653.  Kazusa-KIAA-pYO326
    654.  NIH_MGC_403
    655.  NIH_MGC_404
    656.  NIH_MGC_405
    657.  NIH_MGC_406
    658.  NIH_MGC_417
    659.  NIH_MGC_422
    660.  NIH_MGC_423
    661.  NIH_MGC_424
    662.  WTSI-chORF
    663.  WTSI-ohORF

uterus

    664.  NCI_CGAP_Ut1
    665.  NCI_CGAP_Ut2
    666.  NCI_CGAP_Ut3
    667.  NCI_CGAP_Ut4
    668.  NCI_CGAP_Ut7
    669.  NIH_MGC_44
    670.  NIH_MGC_46
    671.  NIH_MGC_71


NCI_CGAP_Lip2

NAME: NCI_CGAP_Lip2
LIB_ID: 470
ORGANISM: human
STAGE: adult
ORGAN: adipose
TISSUE: liposarcoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from liposarcoma, cDNA made by oligo-dT priming. Non- directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_AA1

NAME: NCI_CGAP_AA1
LIB_ID: 426
ORGANISM: human
STAGE: adult
ORGAN: adrenal gland
TISSUE: two pooled adenomas
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Two pooled bulk adrenal adenomas. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.6 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Adr1

NAME: NCI_CGAP_Adr1
LIB_ID: 1469
ORGANISM: human
ORGAN: adrenal gland
TISSUE: neuroblastoma
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Phe1

NAME: NCI_CGAP_Phe1
LIB_ID: 544
ORGANISM: human
STAGE: adult
ORGAN: adrenal gland
TISSUE: pheochromocytoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pheochromocytoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.3 kb. Note: this is a NCI_CGAP Library.
||

NIH_MGC_84

NAME: NIH_MGC_84
LIB_ID: 1710
ORGANISM: human
ORGAN: adrenal gland
TISSUE: adrenal cortex, carcinoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.229 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NCI_CGAP_CLL1

NAME: NCI_CGAP_CLL1
LIB_ID: 976
ORGANISM: human
ORGAN: B-cell
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTGCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GCB0

NAME: NCI_CGAP_GCB0
LIB_ID: 635
ORGANISM: human
STAGE: adult
ORGAN: B-cell
TISSUE: pooled flow-sorted tonsil
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Germinal center B-cells Library constructed by Dr. L. Staudt (NCI). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GCB1

NAME: NCI_CGAP_GCB1
LIB_ID: 361
ORGANISM: human
STAGE: adult
ORGAN: B-cell
TISSUE: flow-sorted tonsils
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from germinal B-cells (flow-sortedfrom tonsils) provided by Dr. Louis Staudt of the NCI, and was then primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTCATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. This library was previously referred to as Soares NbHTGBC. Note: this is a NCI_CGAP Library.
||

NIH_MGC_48

NAME: NIH_MGC_48
LIB_ID: 1655
ORGANISM: human
ORGAN: B-cell
TISSUE: primary B-cells from tonsils, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_53

NAME: NIH_MGC_53
LIB_ID: 1581
ORGANISM: human
ORGAN: bladder
TISSUE: carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
'Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.55 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_93

NAME: NIH_MGC_93
LIB_ID: 1729
ORGANISM: human
ORGAN: bladder
TISSUE: transitional cell papilloma, cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.7 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_278

NAME: NIH_MGC_278
LIB_ID: 2217
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: Blastocyst
TISSUE: pluripotent cell line derived from blastocyst inner cell mass
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
RNA obtained from pluripotent cell line derived from blastocyst inner cell mass (cell line HSF-1.14, NIH Registry designation UC01. Positive for OCT4 expression by rtPCR, positive for SSEA-3, SSEA-4, Tra-1-81, Tra-1-60 by immunofluorescence. Negative for SSEA-1 by immunofluorescence. Passage 35. This line is a subclone of the parental line; the parental line was subcloned to remove aneuploid cells). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.9 kb. This primary library is non-normalized (normalized primary library is NIH_MGC_279) and was constructed by Express Genomics (Frederick, MD). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_279

NAME: NIH_MGC_279
LIB_ID: 2218
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: Blastocyst
TISSUE: pluripotent cell line derived from blastocyst inner cell mass
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
RNA obtained from pluripotent cell line derived from blastocyst inner cell mass (cell line HSF-1.14, NIH Registry designation UC01. Positive for OCT4 expression by rtPCR, positive for SSEA-3, SSEA-4, Tra-1-81, Tra-1-60 by immunofluorescence. Negative for SSEA-1 by immunofluorescence. Passage 35. This line is a subclone of the parental line; the parental line was subcloned to remove aneuploid cells). cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.82 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_278) and was constructed by Express Genomics (Frederick, MD). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_280

NAME: NIH_MGC_280
LIB_ID: 2219
ORGANISM: human
SEX: female
STAGE: embryo
ORGAN: Blastocyst
TISSUE: pluripotent cell line derived from blastocyst inner cell mass
HOST: DH10B
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
RNA obtained from pluripotent cell line derived from blastocyst inner cell mass (cell line HSF-6, NIH Registry designation UC06. Positive for OCT4 expression by rtPCR, positive for SSEA-3, SSEA-4, Tra-1-81, Tra-1-60 by immunofluorescence. Negative for SSEA-1 by immunofluorescence Passage 62. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.8 kb. This primary library is non-normalized (normalized primary library is NIH_MGC_281) and was constructed by Express Genomics (Frederick, MD). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_281

NAME: NIH_MGC_281
LIB_ID: 2220
ORGANISM: human
SEX: female
STAGE: embryo
ORGAN: Blastocyst
TISSUE: pluripotent cell line derived from blastocyst inner cell mass
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
RNA obtained from pluripotent cell line derived from blastocyst inner cell mass (cell line HSF-6, NIH Registry designation UC06. Positive for OCT4 expression by rtPCR, positive for SSEA-3, SSEA-4, Tra-1-81, Tra-1-60 by immunofluorescence. Negative for SSEA-1 by immunofluorescence Passage 62. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 2.0 kb. This primary library is normalized (non-normalized primary library is NIH_MGC_280) and was constructed by Express Genomics (Frederick, MD). Note: this is a Mammalian Gene Collection library.
||

NCI_CGAP_CML1

NAME: NCI_CGAP_CML1
LIB_ID: 1360
ORGANISM: human
ORGAN: blood
TISSUE: myeloid cells, 18 pooled CML cases, BCR/ABL rearrangement positive, includes both chronic phase and myeloid blast crisis
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies, catalog #11998-010. Note: this is a NCI_CGAP Library.
||

NIH_MGC_106

NAME: NIH_MGC_106
LIB_ID: 1756
ORGANISM: human
ORGAN: blood
TISSUE: natural killer cells, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_2

NAME: NIH_MGC_2
LIB_ID: 1418
ORGANISM: human
ORGAN: blood
TISSUE: blood from T-cell leukemia, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7a
V_TYPE: plasmid
RE_5': CeuI
RE_3': SceI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into CeuI/SceI sites using the following 5'' adaptor: taactataacggtcctaaggtagcga and 3'' adaptor: tttcattacctctttctccgcaccccacataaa. Average insert size 700 bp. Library prepared by Edge BioSystems. Note: this is a NIH_MGC Library. '
||

Soares NPBMC

NAME: Soares NPBMC
LIB_ID: 1659
ORGANISM: human
ORGAN: blood
TISSUE: lymphocytes
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT)primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized; constructed in the laboratory of M. Bento Soares (University of Iowa).
||

NCI_CGAP_DF0

NAME: NCI_CGAP_DF0
LIB_ID: 1913
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: subchondral bone
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTAAGCGTCTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_DF1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_DF1

NAME: NCI_CGAP_DF1
LIB_ID: 1914
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: subchondral bone
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTAAGCGTCTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (non-normalized version of the library is NCI_CGAP_DF0), and was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_ED0

NAME: NCI_CGAP_ED0
LIB_ID: 1920
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: chondrosarcoma, left pubic bone, cell line CS5
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTCAAGGCTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_ED1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_ED1

NAME: NCI_CGAP_ED1
LIB_ID: 1921
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: chondrosarcoma, left pubic bone, cell line CS5
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTCAAGGCTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (non-normalized version of the library is NCI_CGAP_ED0), and was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_EI0

NAME: NCI_CGAP_EI0
LIB_ID: 1922
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: chondrosarcoma, left pelvis
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTGCACTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_EI1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_EI1

NAME: NCI_CGAP_EI1
LIB_ID: 1923
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: chondrosarcoma, left pelvis
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACACTTGCACTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (non-normalized version of the library is NCI_CGAP_EI0), and was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_Ew1

NAME: NCI_CGAP_Ew1
LIB_ID: 475
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: Ewing`s sarcoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from Ewing's sarcoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_FG0

NAME: NCI_CGAP_FG0
LIB_ID: 2080
ORGANISM: human
ORGAN: bone
TISSUE: enchondroma, pool of 2
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCGGTCACTCTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_FG1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FG1

NAME: NCI_CGAP_FG1
LIB_ID: 2081
ORGANISM: human
ORGAN: bone
TISSUE: enchondroma, pool of 2
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCGGTCACTCTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_FG0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_Fs1

NAME: NCI_CGAP_Fs1
LIB_ID: 1924
ORGANISM: human
ORGAN: bone
TISSUE: fibrosarcoma, cell line HT-1088 (ATCC #CCL-121)
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGTTCTACGAGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_Pr12

NAME: NCI_CGAP_Pr12
LIB_ID: 468
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: bone
TISSUE: prostate tumor metastasis to bone
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from metastatic prostate lesion of the bone, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_SS1

NAME: NCI_CGAP_SS1
LIB_ID: 542
ORGANISM: human
STAGE: adult
ORGAN: bone
TISSUE: synovial sarcoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Synovial sarcoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.4 kb. Note: this is a NCI_CGAP Library.
||

NIH_MGC_86

NAME: NIH_MGC_86
LIB_ID: 1712
ORGANISM: human
ORGAN: bone
TISSUE: osteosarcoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.533 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

Jia bone marrow stroma

NAME: Jia bone marrow stroma
LIB_ID: 528
ORGANISM: human
SEX: both
STAGE: mixed
ORGAN: bone marrow
TISSUE: stroma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
mRNA made from human bone marrow stroma, cDNA made by oligo-dT priming. Directionally cloned. Size-selected for average insert size 0.5 kb. Library constructed by Dr. Libin Jia (NHGRI).
||

NCI_CGAP_HSC1

NAME: NCI_CGAP_HSC1
LIB_ID: 541
ORGANISM: human
STAGE: adult
ORGAN: bone marrow
TISSUE: flow-sorted, CD34+/CD38- hematopoietic stem cells
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from flow-sorted CD34+/CD38- hematopoietic stem cells, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HSC3

NAME: NCI_CGAP_HSC3
LIB_ID: 1324
ORGANISM: human
SEX: unknown
ORGAN: bone marrow
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from lymphoid tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HSC4

NAME: NCI_CGAP_HSC4
LIB_ID: 1325
ORGANISM: human
SEX: unknown
ORGAN: bone marrow
TISSUE: stem cell CD34+, CD38-
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from lymphoid tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIH_MGC_54

NAME: NIH_MGC_54
LIB_ID: 1582
ORGANISM: human
ORGAN: bone marrow
TISSUE: chronic myelogenous leukemia, cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.75 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_55

NAME: NIH_MGC_55
LIB_ID: 1583
ORGANISM: human
ORGAN: bone marrow
TISSUE: acute myelogenous leukemia cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NCI_CGAP_Mel1

NAME: NCI_CGAP_Mel1
LIB_ID: 738
ORGANISM: human
ORGAN: bowel
TISSUE: metastatic melanoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Life Technologies catalog #: 10980-019 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Mel3

NAME: NCI_CGAP_Mel3
LIB_ID: 785
ORGANISM: human
ORGAN: bowel
TISSUE: metastatic melanoma to bowel
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.9 kb. Life Technologies catalog #: 10981-017 Note: this is a NCI_CGAP Library.
||

COGENE 4AR (4EAR)

NAME: COGENE 4AR (4EAR)
LIB_ID: 1779
ORGANISM: human
STAGE: embryo
ORGAN: brain/CNS
TISSUE: anterior rhombomeres (5-8) pooled from 4 embryos.
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.3-1.6 kb. Normalized to Cot10. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

COGENE PR (4EPR)

NAME: COGENE PR (4EPR)
LIB_ID: 1778
ORGANISM: human
STAGE: embryo
ORGAN: brain/CNS
TISSUE: posterior rhombomeres (5-8) pooled from 4 embryos.
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-2.0 kb. Normalized to Cot10. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

Clontech brain (HL3002s)

NAME: Clontech brain (HL3002s)
LIB_ID: 69
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

Clontech fetal brain (HL3025s)

NAME: Clontech fetal brain (HL3025s)
LIB_ID: 73
ORGANISM: human
STAGE: fetal
ORGAN: brain/CNS
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

Johnston frontal cortex

NAME: Johnston frontal cortex
LIB_ID: 742
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex, pool of 4
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Stanley Neuropathology Consortium (www.stanleylab.org) brains S-58, S-65, S-67, S-78. Random + oligo-dT primed into EcoRI site of ZAP II Vector. Mass excised. Avg insert length 1.9kb. Non-directionally cloned. Custom library provided by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Lehrach HBF

NAME: Lehrach HBF
LIB_ID: 80
ORGANISM: human
STAGE: fetal
ORGAN: brain/CNS
HOST: XL1
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
directionally cloned
||

Life Tech brain (10418-010)

NAME: Life Tech brain (10418-010)
LIB_ID: 110
ORGANISM: human
SEX: female
STAGE: adult
AGE: 36
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH12S
VECTOR: pCMV-SPORT
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
directionally cloned, average insert size 1.38 kb
||

NCI_CGAP_Brn20

NAME: NCI_CGAP_Brn20
LIB_ID: 1306
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: oligodendroglioma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from oligodendroglioma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. CITATION: National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index CONT_NAME: Robert Strausberg, Ph.D. COMMENT: cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: I.M.A.G.E. Consortium, LLNL DNA Sequencing by: Washington University Genome Sequencing Center Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn21

NAME: NCI_CGAP_Brn21
LIB_ID: 1326
ORGANISM: human
SEX: unknown
ORGAN: brain/CNS
TISSUE: oligodendroglioma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from brain tumor tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn23

NAME: NCI_CGAP_Brn23
LIB_ID: 965
ORGANISM: human
ORGAN: brain/CNS
TISSUE: glioblastoma, pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATATCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn25

NAME: NCI_CGAP_Brn25
LIB_ID: 1110
ORGANISM: human
ORGAN: brain/CNS
TISSUE: anaplastic oligodendroglioma
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATAGGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized, and was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn35

NAME: NCI_CGAP_Brn35
LIB_ID: 1273
ORGANISM: human
ORGAN: brain/CNS
TISSUE: pool of 5 tumors: meningioma, oligodendroglioma, astrocytoma (grade II), medulloblastoma, astrocytoma (grade IV)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.33 kb. Tumor types include: meningioma, oligodendroglioma, astrocytoma (grade II), medulloblastoma, astrocytoma (grade IV). Life Technologies catalog #: 11544-012 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn41

NAME: NCI_CGAP_Brn41
LIB_ID: 1458
ORGANISM: human
ORGAN: brain/CNS
TISSUE: oligodendroglioma
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCACTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed and normalized by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn50

NAME: NCI_CGAP_Brn50
LIB_ID: 1370
ORGANISM: human
ORGAN: brain/CNS
TISSUE: medulloblastoma
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from medulloblastoma tumor tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCTTTCGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. This library is normalized. Library constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn52

NAME: NCI_CGAP_Brn52
LIB_ID: 1331
ORGANISM: human
ORGAN: brain/CNS
TISSUE: pool of 5 tumors: meningioma, oligodendroglioma, astrocytoma (grade II), medulloblastoma, astrocytoma (grade IV)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This library represents the normalized version of NCI_CGAP_Brn35. Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.19 kb. Tumor types include: meningioma, oligodendroglioma, astrocytoma (grade II), medulloblastoma, astrocytoma (grade IV). Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn53

NAME: NCI_CGAP_Brn53
LIB_ID: 1356
ORGANISM: human
ORGAN: brain/CNS
TISSUE: meningioma, pool of 3
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies, catalog #12005-013. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn64

NAME: NCI_CGAP_Brn64
LIB_ID: 1668
ORGANISM: human
ORGAN: brain/CNS
TISSUE: glioblastoma, with EGFR amplification
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.57 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn65

NAME: NCI_CGAP_Brn65
LIB_ID: 1669
ORGANISM: human
ORGAN: brain/CNS
TISSUE: glioblastoma, without EGFR amplification
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.77 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn66

NAME: NCI_CGAP_Brn66
LIB_ID: 1670
ORGANISM: human
ORGAN: brain/CNS
TISSUE: brain, glioblastoma with probable TP53 mutation and no EGFR amplification
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.64 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn67

NAME: NCI_CGAP_Brn67
LIB_ID: 1671
ORGANISM: human
ORGAN: brain/CNS
TISSUE: anaplastic oligodendroglioma with 1p/19q loss
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.3 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Brn70

NAME: NCI_CGAP_Brn70
LIB_ID: 1667
ORGANISM: human
ORGAN: brain/CNS
TISSUE: anaplastic oligodendroglioma
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.ccdb
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.9 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_CNS1

NAME: NCI_CGAP_CNS1
LIB_ID: 730
ORGANISM: human
ORGAN: brain/CNS
TISSUE: substantia nigra
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.0 kb. Life Technologies catalog #: 10979-011 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pit1

NAME: NCI_CGAP_Pit1
LIB_ID: 1358
ORGANISM: human
ORGAN: brain/CNS
TISSUE: pituitary adenoma, 4 pooled
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.7 kb. Library constructed by Life Technologies, catalog #12001-012. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Sch1

NAME: NCI_CGAP_Sch1
LIB_ID: 425
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: brain/CNS
TISSUE: Schwannoma tumors - two bulk tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Two pooled bulk Schwannoma tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb. Note: this is a NCI_CGAP Library.
||

NICHD_HS_Ut1

NAME: NICHD_HS_Ut1
LIB_ID: 1892
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Cloned unidirectionally from microquantity amounts of mRNA from normal endometrial tissue (late proliferative phase, cycle day 13). Average insert size 1.9 kb. Library constructed by ResGen (Invitrogen Corporation).
||

NICHD_HS_Ut2

NAME: NICHD_HS_Ut2
LIB_ID: 1893
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Cloned unidirectionally from microquantity amounts of mRNA from normal endometrial tissue (mid-secretory phase, cycle day 23). Average insert size 1.6 kb. Library constructed by ResGen (Invitrogen Corporation).
||

NIH_MGC_114

NAME: NIH_MGC_114
LIB_ID: 1783
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: pool of 6 brains
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
'RNA source anonymous pool of 6 male brains, age range 23-27 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 019. Note: this is a NIH_MGC Library. '
||

NIH_MGC_119

NAME: NIH_MGC_119
LIB_ID: 1788
ORGANISM: human
SEX: male
STAGE: adult
AGE: 27
ORGAN: brain/CNS
TISSUE: normal medulla
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source normal medulla from anonymous male age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.3 kb, insert size range 0.9-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 013. Note: this is a NIH_MGC Library. '
||

NIH_MGC_121

NAME: NIH_MGC_121
LIB_ID: 1790
ORGANISM: human
SEX: both
STAGE: fetal
ORGAN: brain/CNS
TISSUE: pool of 3 fetal brains
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source anonymous pool of 3 fetal brains, female age 20 weeks, female age 24 weeks, and male age 26 weeks. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 0.7-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 017. Note: this is a NIH_MGC Library. '
||

NIH_MGC_124

NAME: NIH_MGC_124
LIB_ID: 1861
ORGANISM: human
SEX: male
STAGE: adult
AGE: 27
ORGAN: brain/CNS
TISSUE: hippocampus
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source male hippocampus, age 27. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 0.9-4 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 012. Note: this is a NIH_MGC Library. '
||

NIH_MGC_126

NAME: NIH_MGC_126
LIB_ID: 1889
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5'' and 3'' adaptors were used in cloning as follows: 5''-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'' and 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3''. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.5-1 kb size fraction (other fractions present in NIH_MGC_127 and NIH_MGC_128). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library. '
||

NIH_MGC_127

NAME: NIH_MGC_127
LIB_ID: 1890
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5'' and 3'' adaptors were used in cloning as follows: 5''-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'' and 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3''. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 1-2 kb size fraction (other fractions present in NIH_MGC_126 and NIH_MGC_128). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library. '
||

NIH_MGC_128

NAME: NIH_MGC_128
LIB_ID: 1891
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5'' and 3'' adaptors were used in cloning as follows: 5''-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3'' and 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3''. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the >2 kb size fraction (other fractions present in NIH_MGC_126 and NIH_MGC_127). Library created in the laboratory of T. Usdin, M.D., Ph.D. (NIMH, NIH). Note: this is a NIH_MGC Library. '
||

NIH_MGC_141

NAME: NIH_MGC_141
LIB_ID: 1958
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.2-0.5 kb size fraction (other fractions present in NIH_MGC_142). Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library.
||

NIH_MGC_142

NAME: NIH_MGC_142
LIB_ID: 1959
ORGANISM: human
SEX: both
STAGE: embryo
ORGAN: brain/CNS
TISSUE: normal brain, pooled
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Double-stranded cDNA was prepared from a pool of 40 cell line polyA+ RNAs (bladder - 2%, blood - 33.4%, brain - 5.6%, breast - 12.5%, colon - 4%, connective tissue - 1.4%, eye - 1%, intestine - 2.6%, kidnney - 2.2%, liver - 5.7%, lung - 10.8%, NK-cell - 5.2%, ovary - 4%, pharynx - 2.5%, prostate - 4.3%, salivary gland - 1.3%, and skin - 2.3%). 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the >0.5 kb size fraction (other fractions present in NIH_MGC_141). Library created in the laboratory of M. Brownstein (NIMH, NIH).
||

NIH_MGC_181

NAME: NIH_MGC_181
LIB_ID: 2026
ORGANISM: human
ORGAN: brain/CNS
TISSUE: White matter
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.42 kb. Library was constructed by Invitrogen. Note: this is a NIH_MGC Library. '
||

NIH_MGC_19

NAME: NIH_MGC_19
LIB_ID: 1476
ORGANISM: human
ORGAN: brain/CNS
TISSUE: neuroblastoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_192

NAME: NIH_MGC_192
LIB_ID: 2050
ORGANISM: human
ORGAN: brain/CNS
TISSUE: glioblastoma cell line
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': SmaI
RE_3': NotI
DESCR:
The library was constructed by reverse transcription of 1 ug mRNA using the oligo dT primer GCGGCCGCCC(T)20 and an RNaseH + MMLV reverse transcriptase. Second strand synthesis was carried out by standard methods. The cDNA was size selected by agarose gel for > 1.2 kb, digested with Not I and directionally cloned into the vector Express-1 at the SmaI/NotI sites. DNA from the primary library was used for in vitro transcription from the T7 promoter to produce biotinylated RNA transcripts. These biotinylated transcripts, along with blocking oligos to the poly-A, multiple cloning site and primer regions, were hybridized with single stranded circles produced by phagmeid production from the primary library to a Cot value of 10-20. Strepavidin/phenol extraction was utilized to remove DNA:RNA hybrids leaving un-hybridized single stranded circles which were repaired by primer extension and transformed back into E. coli resulting in the normalized library. Average insert size 2.0 kb. 3' linker/adaptor sequence GCGGCCGCCC(T)20. This libary was constructed by Agencourt Bioscience.
||

NIH_MGC_227

NAME: NIH_MGC_227
LIB_ID: 2090
ORGANISM: human
SEX: male
STAGE: adult
AGE: 47
ORGAN: brain/CNS
TISSUE: Bulk tissue from Human Spinal cord
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)30N-1N-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.3kb Library created in the laboratory of Jonathan Kuo and Ted Usdin. Note: this is a NIH_MGC Library
||

NIH_MGC_228

NAME: NIH_MGC_228
LIB_ID: 2091
ORGANISM: human
SEX: male
STAGE: adult
AGE: 47
ORGAN: brain/CNS
TISSUE: cerebellar cortex
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed and directionally cloned.5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)3N-1N-3. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.2kb Library created in the laboratory of Jonathan Kuo and Ted Usdin.
||

NIH_MGC_229

NAME: NIH_MGC_229
LIB_ID: 2092
ORGANISM: human
SEX: male
STAGE: adult
AGE: 47
ORGAN: brain/CNS
TISSUE: Human Brain - Frontal Cortex
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed and directionally cloned.5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-d(T)3N-1N-3. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected for >0.5kb with an average insert size of 1.2kb Library created in the laboratory of Jonathan Kuo and Ted Usdin.
||

NIH_MGC_264

NAME: NIH_MGC_264
LIB_ID: 2170
ORGANISM: human
SEX: female
STAGE: adult
AGE: 73
ORGAN: brain/CNS
TISSUE: normal brain
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_272 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_266

NAME: NIH_MGC_266
LIB_ID: 2172
ORGANISM: human
SEX: both
STAGE: fetal
ORGAN: brain/CNS
TISSUE: normal brain, pool
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_274 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_272

NAME: NIH_MGC_272
LIB_ID: 2204
ORGANISM: human
SEX: female
STAGE: adult
AGE: 73
ORGAN: brain/CNS
TISSUE: normal brain
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_264 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_274

NAME: NIH_MGC_274
LIB_ID: 2206
ORGANISM: human
SEX: both
STAGE: fetal
ORGAN: brain/CNS
TISSUE: normal brain, pool
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_266 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_286

NAME: NIH_MGC_286
LIB_ID: 2260
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebral cortex
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_287 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_287

NAME: NIH_MGC_287
LIB_ID: 2261
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebral cortex
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_286 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_288

NAME: NIH_MGC_288
LIB_ID: 2282
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebral cortex
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_289 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_289

NAME: NIH_MGC_289
LIB_ID: 2283
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebral cortex
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_288 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_306

NAME: NIH_MGC_306
LIB_ID: 2270
ORGANISM: human
ORGAN: brain/CNS
TISSUE: normal brain
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_307 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_307

NAME: NIH_MGC_307
LIB_ID: 2271
ORGANISM: human
ORGAN: brain/CNS
TISSUE: normal brain
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_306 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_308

NAME: NIH_MGC_308
LIB_ID: 2292
ORGANISM: human
ORGAN: brain/CNS
TISSUE: normal brain
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_309 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_309

NAME: NIH_MGC_309
LIB_ID: 2293
ORGANISM: human
ORGAN: brain/CNS
TISSUE: normal brain
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_308 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_310

NAME: NIH_MGC_310
LIB_ID: 2272
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebellum
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_311 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_311

NAME: NIH_MGC_311
LIB_ID: 2273
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebellum
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_310 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_312

NAME: NIH_MGC_312
LIB_ID: 2294
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebellum
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_313 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_313

NAME: NIH_MGC_313
LIB_ID: 2295
ORGANISM: human
ORGAN: brain/CNS
TISSUE: cerebellum
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_312 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_47

NAME: NIH_MGC_47
LIB_ID: 1747
ORGANISM: human
ORGAN: brain/CNS
TISSUE: neuroblastoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_56

NAME: NIH_MGC_56
LIB_ID: 1584
ORGANISM: human
ORGAN: brain/CNS
TISSUE: primitive neuroectodermal cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_57

NAME: NIH_MGC_57
LIB_ID: 1585
ORGANISM: human
ORGAN: brain/CNS
TISSUE: glioblastoma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.55 kb (range 0.9-4.0 kb). 12/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_73

NAME: NIH_MGC_73
LIB_ID: 1641
ORGANISM: human
ORGAN: brain/CNS
TISSUE: brain
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.35 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_95

NAME: NIH_MGC_95
LIB_ID: 1698
ORGANISM: human
ORGAN: brain/CNS
TISSUE: hippocampus tissue
HOST: DH10B
VECTOR: pBluescriptR
V_TYPE: phagemid
RE_5': SalI-XhoI
RE_3': BamHI
DESCR:
' Oligo-dT primed using primer 5''-TTTTTTTTTTTTTTTTVN-3'', size-selected for average insert size 2.5 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library. '
||

NIH_MGC_96

NAME: NIH_MGC_96
LIB_ID: 1708
ORGANISM: human
ORGAN: brain/CNS
TISSUE: hypothalamus tissue
HOST: DH10B
VECTOR: pBluescriptR
V_TYPE: phagemid
RE_5': SalI-XhoI
RE_3': BamHI
DESCR:
' Oligo-dT primed using primer 5''-TTTTTTTTTTTTTTTTVN-3'', size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library. '
||

NIH_MGC_98

NAME: NIH_MGC_98
LIB_ID: 1766
ORGANISM: human
ORGAN: brain/CNS
TISSUE: astrocytoma, grade IV, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

Schiller astrocytoma

NAME: Schiller astrocytoma
LIB_ID: 1278
ORGANISM: human
SEX: male
STAGE: adult
AGE: 44
ORGAN: brain/CNS
TISSUE: astrocytoma, determined by consensus pathology
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from human astrocytoma usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
||

Schiller glioblastoma multiforme

NAME: Schiller glioblastoma multiforme
LIB_ID: 1280
ORGANISM: human
SEX: female
STAGE: adult
AGE: 33
ORGAN: brain/CNS
TISSUE: glioblastoma multiforme, determined by consensus pathology. Tumor has LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C.
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from human glioblastoma multiforme usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; shows LOH on chromosomes 10 and 14 and a gain in 9p and 1q. The tumor also has a heterozygous p53 mutation R273C. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
||

Schiller meningioma

NAME: Schiller meningioma
LIB_ID: 1279
ORGANISM: human
SEX: female
STAGE: adult
AGE: 72
ORGAN: brain/CNS
TISSUE: meningioma, determined by consensus pathology
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from human meningioma usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
||

Schiller oligodendroglioma

NAME: Schiller oligodendroglioma
LIB_ID: 1277
ORGANISM: human
SEX: male
STAGE: adult
AGE: 44
ORGAN: brain/CNS
TISSUE: oligodendroglioma, determined by consensus pathology and chromosome 1p and 19q deletion determined by CGH.
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from human oligodendroglioma usingprimer 5'-GAGAGAGAGAGAGAGAGAGAAACTAGTCTGAGT(18)-3'. An EcoRI adaptor was used on the 5' end of the cDNA as follows: 5'-AATTCGGCACGAG-3'. The library was size-selected and went through one round of amplification. Average insert size is 1.7 kb, with a range from 0.4-12 kb. Tumor identification by consensus pathology; contains chromosome 1p and 19q deletion as determined by CGH. This library was constructed by Dr. Martin Schiller (Johns Hopkins University).
||

Schneider fetal brain 00004

NAME: Schneider fetal brain 00004
LIB_ID: 1341
ORGANISM: human
SEX: male
STAGE: fetal
ORGAN: brain/CNS
TISSUE: frontal lobe
HOST: DH10B
VECTOR: pBluescript SK+
V_TYPE: phagemid
RE_5': SstI
RE_3': XhoI
DESCR:
Double-stranded cDNA was prepared from human fetal brain tissue. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-GAGAGAGAGAAGAGCTCAAGGATCCTTAATTAAATTAATCCCCCCCCCCCCC-3' and 3' adaptor sequence: 5'-GAGAGAGAGACTCGAGTTTTTTTTTTTTTTTTTT-3'. The library was size-selected for >0.5 kb inserts and has an average insert size estimated at 1.2 kb. This library was constructed using the CAP-trapper method for full-length enrichment and has not undergone amplification. Library was constructed by Dr. Claudio Schneider (LNCIB-Area Science Park, Trieste, Italy).
||

Soares 1NIB

NAME: Soares 1NIB
LIB_ID: 52
ORGANISM: human
SEX: female
STAGE: infant
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B
VECTOR: Lafmid BA
V_TYPE: phagemid
RE_5': HindIII
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Hind III adaptors (Pharmacia), digested with Not I and directionally cloned into the Not I and Hind III sites of the Lafmid BA vector. Library went through one round of normalization. Reference: PNAS 91, 9228-9232 (1994) Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares 2NbHMSP

NAME: Soares 2NbHMSP
LIB_ID: 201
ORGANISM: human
SEX: male
STAGE: adult
AGE: 46
ORGAN: brain/CNS
TISSUE: multiple sclerosis plaque
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA (prepared from RNA from 4 multiple sclerosis lesions from one patient, provided by Dr. K.G. Becker, NINDS/NIH) was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot5. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares N2b4HB55Y

NAME: Soares N2b4HB55Y
LIB_ID: 83
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot50, and contains inserts larger than 1.5 kb. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
||

Soares N2b5HB55Y

NAME: Soares N2b5HB55Y
LIB_ID: 84
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot50, and contains inserts ranging from 0.5 to 1.5 kb. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
||

Soares NbHFB

NAME: Soares NbHFB
LIB_ID: 423
ORGANISM: human
STAGE: fetal
ORGAN: brain/CNS
TISSUE: whole brain
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' AACTGGAAGAATTCGCGGCCGCAATATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Stanley Frontal B/N pool 1

NAME: Stanley Frontal B/N pool 1
LIB_ID: 1252
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: S-136 (35 yo female), S-65 (48 yo male), S-75 (bipolar 34 yo male), S-83 (biplolar 48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male bipolars, ages 34 and 48 (S-75, S-83) subtracted by pool of two mentally normal individuals, male, age 48 and female, age 35 (S-65, S-136). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal B/N pool 2

NAME: Stanley Frontal B/N pool 2
LIB_ID: 1255
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: S-111 (bipolar 45 yo male), S-124 (41 yo male), S-128 (bipolar 50 yo male), S-141 (53 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two bipolar males, ages 45 and 50 (S-111, S-128) subtracted by pool of two mentally normal males, ages 41 and 53 (S-124, S-141). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal N/B pool 2

NAME: Stanley Frontal N/B pool 2
LIB_ID: 1256
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: S-111 (bipolar 45 yo male), S-124 (41 yo male), S-128 (bipolar 50 yo male), S-141 (53 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two mentally normal males, ages 41 and 53 (S-124, S-141) subtracted by pool of two bipolar males, ages 45 and 50 (S-111, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal N/S pool 2

NAME: Stanley Frontal N/S pool 2
LIB_ID: 1254
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: schizophrenic 44 yo male, S-118 (56 yo female), S-124 (41 yo male), S-141 (53 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two mentally normal male individuals ages 41 and 53 (S-124, S-141) subtracted by pool of two schizophrenic individuals, male age 44 and female age 56 (S-116, S-118). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal S/B pool 1

NAME: Stanley Frontal S/B pool 1
LIB_ID: 1251
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: S-121 (schizophrenic 32 yo male), S-30 (schizophrenic 30 yo male), S-75 (bipolar 34 yo male), S-83 (bipolar 48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male schizophrenics, ages 30 and 32 (S-30, S-121) subtracted by pool of two male bipolars, age 34 and 48 (S-75, S-83). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal S/N individual

NAME: Stanley Frontal S/N individual
LIB_ID: 1249
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: brain/CNS
TISSUE: pool of 2 frontal cortexes: S-11 (schizophrenic 34 yo male), S-37 (28 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. 34 yo schizophrenic male (S-11) subtracted by 28 yo mentally normal male (S-37). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal S/N pool 1

NAME: Stanley Frontal S/N pool 1
LIB_ID: 1250
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: S-121 (schizophrenic 32 yo male), S-136 (35 yo female), S-30 (schizophrenic 30 yo male), S-65 (48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two male schizophrenics, ages 30 and 32 (S-30, S-121) subtracted by pool of two mentally normal individuals, male, age 48 and female, age 35 (S-65, S-136). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Frontal S/N pool 2

NAME: Stanley Frontal S/N pool 2
LIB_ID: 1253
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: frontal cortex pool: schizophrenic 44 yo male, S-118 (schizophrenic 56 yo female), S-124 (41 yo male), S-141 (53 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of two schizophrenics, male age 44 and female age 56 (S-116, S-118) subtracted by pool of two mentally normal male individuals ages 41 and 53 (S-124, S-141). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Hippocampus B/N pool 1

NAME: Stanley Hippocampus B/N pool 1
LIB_ID: 1259
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: hippocampus pool: S-128 (bipolar 50 yo male), S-164 (50 yo female), S-47 (bipolar 37 yo female), S-65 (48 yo male), S-67 (34 yo male), S-83 (bipolar 48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128) subtracted by pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Hippocampus B/S pool 1

NAME: Stanley Hippocampus B/S pool 1
LIB_ID: 1262
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: hippocampus pool: S-109 (schizophrenic 60 yo male), S-11 (schizophrenic 34 yo male), S-128 (bipolar 50 yo male), S-47 (bipolar 37 yo female), S-64 (schizophrenic 58 yo male), S-83 (biploar 48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128) subtracted by pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Hippocampus N/B pool 1

NAME: Stanley Hippocampus N/B pool 1
LIB_ID: 1260
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: hippocampus pool: S-128 (bipolar 50 yo male), S-164 (50 yo female), S-47 (bipolar 37 yo female), S-65 (48 yo male), S-67 (34 yo male), S-83 (bipolar 48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164) subtracted by pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Hippocampus N/S pool 1

NAME: Stanley Hippocampus N/S pool 1
LIB_ID: 1258
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: hippocampus pool: S-109 (schizophrenic 60 yo male), S-11 (schizophrenic 34 yo male), S-164 (50 yo female), S-64 (schizophrenic 58 yo male), S-65 (48 yo male), S-67 (34 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164) subtracted by pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Hippocampus S/B pool 1

NAME: Stanley Hippocampus S/B pool 1
LIB_ID: 1261
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: hippocampus pool: S-109 (schizophrenic 60 yo male), S-11 (schizophrenic 34 yo male), S-128 (bipolar 50 yo male), S-47 (bipolar 37 yo female), S-64 (schizophrenic 58 yo male), S-83 (bipolar 48 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109) subtracted by pool of three bipolar individuals, male, ages 48 and 50, female, age 37 (S-47, S-83, S-128). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stanley Hippocampus S/N pool 1

NAME: Stanley Hippocampus S/N pool 1
LIB_ID: 1257
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: brain/CNS
TISSUE: hippocampus pool: S-109 (schizophrenic 60 yo male), S-11 (schizophrenic 34 yo male), S-164 (50 yo female), S-64 (schizophrenic 58 yo male), S-65 (48 yo male), S-67 (34 yo male)
HOST: GeneHogs DH10B
VECTOR: pCR2.1-TOPO
V_TYPE: plasmid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Total RNA (purified with Trizol and DNAsed before use) was reverse transcribed using a modified oligo-dT primer containing RsaI and HindIII sites. Double- stranded cDNA was digested with RsaI, resulting in blunt ended cDNA of an average 0.1-2 kb in length. Digested cDNA was split into two sets, one used as is as the driver, the other set was split in half again and each half linked to a different adaptor (5'-TCGAGCGGCCGCCCGGGCAGGT-3' or 5'- AGGGCGTGGTGCGGAGGGCGGT-3'), to be used as tester. Subtraction was performed using the Clontech PCR Select cDNA subtraction kit. Pool of three schizophrenic males, ages 34, 58 and 60 (S-11, S-64, S-109) subtracted by pool of three mentally normal individuals, male, ages 34 and 48, female, age 50 (S-65, S-67, S-164). Tissues were obtained from the Stanley Neuropathology Consortium (www.stanleylab.org). Library constructed and subtracted by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

Stratagene NT2 neuroepithelium (937230)

NAME: Stratagene NT2 neuroepithelium (937230)
LIB_ID: 278
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: uninduced, exponentially growing neuroepithelial cells (Ntera-2/cl.D1)
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Uninduced, exponentially growing neuroepithelial cells (Ntera-2/c1.D1). Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene NT2/RA neuroepithelium 937231

NAME: Stratagene NT2/RA neuroepithelium 937231
LIB_ID: 279
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: NT2 cells induced with retinoic acid for 24 hours
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. NT2 cells (Ntera-2/c1.D1) induced with Retinoic Acid for 24 hours. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene NT2/RA+MI neuroepith (937234)

NAME: Stratagene NT2/RA+MI neuroepith (937234)
LIB_ID: 281
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: NT2 precursor cells induced with retinoic acid for 1 week, followed by 3 weeks in mitotic inhibitors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. NT2 (Ntera-2/c1.D1) precursor cells induced with Retinoic Acid for 1 week, followed by 3 weeks in mitotic inhibitors (Replate #2). Average insert size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene fetal brain (937226)

NAME: Stratagene fetal brain (937226)
LIB_ID: 64
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: brain/CNS
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
||

Stratagene mature hNT (937233)

NAME: Stratagene mature hNT (937233)
LIB_ID: 280
ORGANISM: human
STAGE: adult
ORGAN: brain/CNS
TISSUE: differentiated, post-mitotic hNT neurons
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Differentiated, post mitotic hNT neurons. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene schizophrenic brain S11

NAME: Stratagene schizophrenic brain S11
LIB_ID: 380
ORGANISM: human
SEX: male
STAGE: adult
AGE: 34
ORGAN: brain/CNS
TISSUE: frontal cortex, schizophrenic
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': EcoRI
DESCR:
Random + oligo-dT primed into EcoRI site of ZAP II Vector. Mass excised. Avg insert length 1.4kb. Non-directionally cloned. Custom library provided by Dr. Nancy Johnston [(410) 614-3918, nlj@welchlink.welch.jhu.edu].
||

NCI_CGAP_Br1.1

NAME: NCI_CGAP_Br1.1
LIB_ID: 501
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: pooled bulk tumor
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAACCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is not normalized. (The normalized version of this library is NCI_CGAP_Br2.) Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br12

NAME: NCI_CGAP_Br12
LIB_ID: 1245
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: invasive breast carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from breast carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Non-amplified library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br13

NAME: NCI_CGAP_Br13
LIB_ID: 1288
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: breast carcinoma in situ
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from breast carcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br14

NAME: NCI_CGAP_Br14
LIB_ID: 1289
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: normal breast epithelium
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from breast epithelium, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. CITATION: National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index CONT_NAME: Robert Strausberg, Ph.D. COMMENT: cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: I.M.A.G.E. Consortium, LLNL Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br15

NAME: NCI_CGAP_Br15
LIB_ID: 1300
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: adenocarcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from breast adenocarcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br16

NAME: NCI_CGAP_Br16
LIB_ID: 1298
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: lobullar carcinoma in situ
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from breast carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br17

NAME: NCI_CGAP_Br17
LIB_ID: 1299
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: adenocarcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from breast adenocarcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br18

NAME: NCI_CGAP_Br18
LIB_ID: 1355
ORGANISM: human
SEX: female
ORGAN: breast
TISSUE: pool of 3 high-grade tumors: 1 primary, 2 metatstatic to ovary
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Library constructed by Life Technologies, catalog #12006-011. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br2

NAME: NCI_CGAP_Br2
LIB_ID: 502
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: pooled bulk tumor
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from pooled bulk breast tumor tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAACCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. This library is the normalized version of NCI_CGAP_Br1.1. Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br22

NAME: NCI_CGAP_Br22
LIB_ID: 1570
ORGANISM: human
SEX: female
ORGAN: breast
TISSUE: invasive ductal carcinoma, 3 pooled samples
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br3

NAME: NCI_CGAP_Br3
LIB_ID: 534
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: ductal tumor
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Ductal breast tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb.
||

NCI_CGAP_Br4

NAME: NCI_CGAP_Br4
LIB_ID: 590
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: ductal tissue
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal breast ductal tissue, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br5

NAME: NCI_CGAP_Br5
LIB_ID: 591
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
TISSUE: infiltrating ductal carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from infiltrating ductal carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Br7

NAME: NCI_CGAP_Br7
LIB_ID: 732
ORGANISM: human
ORGAN: breast
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Life Technologies catalog #:10985-018 Note: this is a NCI_CGAP Library.
||

NIH_MGC_107

NAME: NIH_MGC_107
LIB_ID: 1859
ORGANISM: human
ORGAN: breast
TISSUE: adenocarcinoma, cell line
HOST: DH10B TonA
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_151

NAME: NIH_MGC_151
LIB_ID: 2112
ORGANISM: human
ORGAN: breast
TISSUE: cancer cells growing in a xenograft mouse model
HOST: DH10B
VECTOR: pOTB7-3
V_TYPE: phagemid
RE_5': AscI
RE_3': BstXI
DESCR:
Cells were isolated by flow cytometry and RNA isolated in Trizol reagent. cDNA was obtained by reverse transcription using oligo-dT primer 5'-GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV-3'and treated with ExoI. PCR was performed using a complementary 3' primer containing a BstXI site and a cap-switch 5' primer. The resulting cDNA was digested with BstXI and ligated to a BstXI adaptor 5'-AAAAAAAAAAAAAAAAGTCGACATCGATACGCGTGGTCTCGTAGCTGACTTTCCAGCACA-3' then digested with AscI. Size-selection was performed >0.5 kb to result in an average insert size of 0.8 kb. Library constructed and contributed by Mike Clarke and Andrew Hass (University of Michigan). Note: this is an MGC library.
||

NIH_MGC_87

NAME: NIH_MGC_87
LIB_ID: 1713
ORGANISM: human
SEX: female
ORGAN: breast
TISSUE: mammary adenocarcinoma, cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.383 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

Soares 2NbHBst

NAME: Soares 2NbHBst
LIB_ID: 78
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization to Cot230. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
||

Soares 3NbHBst

NAME: Soares 3NbHBst
LIB_ID: 79
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: breast
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization to Cot20. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
||

NCI_CGAP_Car1

NAME: NCI_CGAP_Car1
LIB_ID: 1912
ORGANISM: human
ORGAN: cartilage
TISSUE: osteoarthritic cartilage from knee
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTGATCACGCTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_EU0

NAME: NCI_CGAP_EU0
LIB_ID: 2074
ORGANISM: human
ORGAN: cartilage
TISSUE: cartilage, osteoarthritic
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCTGATCACGCTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_EU1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_EU1

NAME: NCI_CGAP_EU1
LIB_ID: 2075
ORGANISM: human
ORGAN: cartilage
TISSUE: cartilage, osteoarthritic
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCTGATCACGCTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_EU0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_EZ0

NAME: NCI_CGAP_EZ0
LIB_ID: 2076
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade II
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTAATATGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_EZ1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_EZ1

NAME: NCI_CGAP_EZ1
LIB_ID: 2077
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade II
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTAATATGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_EZ0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FE0

NAME: NCI_CGAP_FE0
LIB_ID: 2078
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade II, pool of 3
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCGCTACGGACTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_FE1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FE1

NAME: NCI_CGAP_FE1
LIB_ID: 2079
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade II, pool of 3
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCGCTACGGACTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_FE0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FH0

NAME: NCI_CGAP_FH0
LIB_ID: 2082
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade I
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAGAATCCGGCTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_FH1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FH1

NAME: NCI_CGAP_FH1
LIB_ID: 2083
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade I
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAGAATCCGGCTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_FH0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FL0

NAME: NCI_CGAP_FL0
LIB_ID: 2084
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade III, pool of 4
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGAGGTCGGTGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_FL1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FL1

NAME: NCI_CGAP_FL1
LIB_ID: 2085
ORGANISM: human
ORGAN: cartilage
TISSUE: chondrosarcoma, grade III, pool of 4
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGAGGTCGGTGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version of this library is also available, NCI_CGAP_FL0). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

Clontech HeLa S3 cells (HL3021s)

NAME: Clontech HeLa S3 cells (HL3021s)
LIB_ID: 75
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: cervix
TISSUE: HeLa S3 cells
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

NIH_MGC_12

NAME: NIH_MGC_12
LIB_ID: 1421
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: carcinoma cell line
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. Library prepared by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_13

NAME: NIH_MGC_13
LIB_ID: 1564
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-NCI
V_TYPE: phagemid
RE_5': SfiI
RE_3': SfiI
DESCR:
'Double-stranded cDNA was prepared from cell line RNA.
5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence:
5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence:
5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.7-3.7 kb).
This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
Note: this is a NIH_MGC Library.
'
||

NIH_MGC_35

NAME: NIH_MGC_35
LIB_ID: 1409
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7a
V_TYPE: plasmid
RE_5': CeuI
RE_3': SceI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into CeuI/SceI sites using the following 5'' adaptor: taactataacggtcctaaggtagcga and 3'' adaptor: tttcattacctctttctccgcaccccacataaa. Average insert size 1.8kb. Library prepared by Edge BioSystems. Note: this is a NIH_MGC Library. '
||

NIH_MGC_4

NAME: NIH_MGC_4
LIB_ID: 1420
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: cervix carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7a
V_TYPE: plasmid
RE_5': CeuI
RE_3': SceI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into CeuI/SceI sites using the following 5'' adaptor: taactataacggtcctaaggtagcga and 3'' adaptor: tttcattacctctttctccgcaccccacataaa. Average insert size 900 bp. Library prepared by Edge BioSystems. Note: this is a NIH_MGC Library. '
||

NIH_MGC_5

NAME: NIH_MGC_5
LIB_ID: 1424
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: cervix carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_64

NAME: NIH_MGC_64
LIB_ID: 1530
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7a
V_TYPE: plasmid
RE_5': CeuI
RE_3': SceI
DESCR:
'This library is a size selection of NIH_MGC_35, from 3.0-4.5 kb. Size selection done at the National Institute of Mental Health, NIH. Note: this is a NIH_MGC Library. '
||

Soares NHCe

NAME: Soares NHCe
LIB_ID: 1383
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: normal cervix
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGGCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized; constructed by Bento Soares and M.Fatima Bonaldo.
||

Soares NHCeC

NAME: Soares NHCeC
LIB_ID: 1384
ORGANISM: human
SEX: female
ORGAN: cervix
TISSUE: cervical tumor
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGAAGTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized; constructed by Bento Soares and M.Fatima Bonaldo.
||

Stratagene HeLa S3 cells (937216)

NAME: Stratagene HeLa S3 cells (937216)
LIB_ID: 272
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: cervix
TISSUE: HeLa S3 epithelioid carcinoma cells grown to semi-confluency with no induction
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. HeLa S3 epithelioid carcinoma cells grown to semi-confluency without induction. Average insert size: 1.5 kb; Uni-ZAP XR Vector. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Morton fetal cochlea

NAME: Morton fetal cochlea
LIB_ID: 190
ORGANISM: human
STAGE: fetal
ORGAN: cochlea
TISSUE: pooled
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Fetal cochlea, normal. 37% of inserts <0.5kb, 56% 0.5-1.0kb, 7% >1kb. Uni-ZAP XR Vector. Library constructed by N. Robertson, C. Morton. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Reference: Genomics 23, 42-50 (1994)
||

Barstead HPL-RB7

NAME: Barstead HPL-RB7
LIB_ID: 1311
ORGANISM: human
SEX: male
STAGE: adult
AGE: 25
ORGAN: colon
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [5' AATTCACTAGTAAT 3' and 5' ATTACTAGTG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

NCI_CGAP_Co1

NAME: NCI_CGAP_Co1
LIB_ID: 406
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: bulk tumor
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co10

NAME: NCI_CGAP_Co10
LIB_ID: 527
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: tumor RER+
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAACGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized. Non-normalized version of this library is named NCI_CGAP_Co9. Library was constructed by Bento Soares and M. Fatima Bonaldo (N-Soares4). Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co11

NAME: NCI_CGAP_Co11
LIB_ID: 532
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: pooled tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Multiple colon tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co12

NAME: NCI_CGAP_Co12
LIB_ID: 543
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: pooled tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pooled colon tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co14

NAME: NCI_CGAP_Co14
LIB_ID: 1240
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: moderately differentiated adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.7 kb. Life Technologies catalog #: 11531-019 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co16

NAME: NCI_CGAP_Co16
LIB_ID: 1296
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: tumor RER+
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Plasmid DNA from the normalized library NCI_CGAP_Co10 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1057416-1061255, and 1144584-1145351). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co17

NAME: NCI_CGAP_Co17
LIB_ID: 1359
ORGANISM: human
ORGAN: colon
TISSUE: juvenile granulosa tumor
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.9 kb. Library constructed by Life Technologies, catalog #11999-018. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co18

NAME: NCI_CGAP_Co18
LIB_ID: 1371
ORGANISM: human
ORGAN: colon
TISSUE: moderately differentiated adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.26 kb. Library constructed by Life Technologies. Normalized versions of this library named NCI_CGAP_Co19 (Cot 50), NCI_CGAP_Co20 (Cot 500), and NCI_CGAP_Co21 (Cot >500). Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co19

NAME: NCI_CGAP_Co19
LIB_ID: 1372
ORGANISM: human
ORGAN: colon
TISSUE: moderately differentiated adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Normalized to Cot50. Average insert size 1.32kb. Normalized version of NCI_CGAP_Co18. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co2

NAME: NCI_CGAP_Co2
LIB_ID: 428
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: bulk villous adenoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Bulk colon villous adenoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co20

NAME: NCI_CGAP_Co20
LIB_ID: 1373
ORGANISM: human
ORGAN: colon
TISSUE: moderately differentiated adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Normalized to Cot 500. Average insert size 1.11kb. Normalized version of NCI_CGAP_Co18. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co21

NAME: NCI_CGAP_Co21
LIB_ID: 1374
ORGANISM: human
ORGAN: colon
TISSUE: moderately differentiated adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Normalized to Cot >500. Average insert size 1.04kb. Normalized version of NCI_CGAP_Co18. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co22

NAME: NCI_CGAP_Co22
LIB_ID: 1386
ORGANISM: human
ORGAN: colon
TISSUE: colonic adenocarcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co23

NAME: NCI_CGAP_Co23
LIB_ID: 1462
ORGANISM: human
ORGAN: colon
TISSUE: colonnic adenocarcinoma metastasis
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from colonic metastasis, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co25

NAME: NCI_CGAP_Co25
LIB_ID: 1603
ORGANISM: human
ORGAN: colon
TISSUE: adenocarcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from colonic adenocarcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co26

NAME: NCI_CGAP_Co26
LIB_ID: 1688
ORGANISM: human
ORGAN: colon
TISSUE: normal colonic mucosa
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal colonic mucosa, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co27

NAME: NCI_CGAP_Co27
LIB_ID: 1691
ORGANISM: human
ORGAN: colon
TISSUE: adenocarcinoma (mucinous component)
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from colonic adenocarcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co28

NAME: NCI_CGAP_Co28
LIB_ID: 1690
ORGANISM: human
ORGAN: colon
TISSUE: normal colonic mucosa
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from colonic mucosa, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co29

NAME: NCI_CGAP_Co29
LIB_ID: 1692
ORGANISM: human
ORGAN: colon
TISSUE: tubulovillous adenoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from colonic adenoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co3

NAME: NCI_CGAP_Co3
LIB_ID: 424
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: 12 pooled bulk tumors
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from 12 pooled bulk tumor samples andprimed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTCGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co4

NAME: NCI_CGAP_Co4
LIB_ID: 505
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: colon
TISSUE: tumor, 2 pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from pooled colon tumor tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTCGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. This library is not normalized. Library constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co8

NAME: NCI_CGAP_Co8
LIB_ID: 723
ORGANISM: human
ORGAN: colon
TISSUE: adenomcarcinoma
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from colon adenomcarcinoma, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized. Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Co9

NAME: NCI_CGAP_Co9
LIB_ID: 526
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: tumor RER+
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from RER+ colon tumor, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAACGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is not normalized. Normalized version of this library is named NCI_CGAP_Co10. Library was constructed by Bento Soares and M. Fatima Bonaldo (Soares4). Note: this is a NCI_CGAP Library.
||

NIH_MGC_15

NAME: NIH_MGC_15
LIB_ID: 1428
ORGANISM: human
ORGAN: colon
TISSUE: colon adenocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_268

NAME: NIH_MGC_268
LIB_ID: 2174
ORGANISM: human
SEX: male
STAGE: adult
AGE: 51
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_276 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_276

NAME: NIH_MGC_276
LIB_ID: 2208
ORGANISM: human
SEX: male
STAGE: adult
AGE: 51
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_268 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_294

NAME: NIH_MGC_294
LIB_ID: 2264
ORGANISM: human
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_295 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_295

NAME: NIH_MGC_295
LIB_ID: 2265
ORGANISM: human
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_294 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_296

NAME: NIH_MGC_296
LIB_ID: 2286
ORGANISM: human
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_297 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_297

NAME: NIH_MGC_297
LIB_ID: 2287
ORGANISM: human
ORGAN: colon
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_296 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_65

NAME: NIH_MGC_65
LIB_ID: 1614
ORGANISM: human
ORGAN: colon
TISSUE: adenocarcinoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

Soares/Dieckgraefe NHCD

NAME: Soares/Dieckgraefe NHCD
LIB_ID: 1336
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: colonic mucosa isolated from patients with moderate to severe Crohn's disease; including perforating (fistulas)and non-perforating samples.
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from moderate to severe Crohn's disease; samples include both perforating (fistulas) and non-perforating samples. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares/Dieckgraefe NHUC

NAME: Soares/Dieckgraefe NHUC
LIB_ID: 1335
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: 5 ulcerative colitis patients - colonic mucosa representing different stages of disease from mild cryptitis to severe ulceration, fibrosis and degeneration.
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCTAGCTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Tissue samples provided by Dr. Brian Dieckgraefe (Washington University, dieck@im.wustl.edu); colonic mucosa represents a range of disease involvement from mild cryptitis to severe ulceration, fibrosis, and degeneration. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Stratagene colon tumor (937204)

NAME: Stratagene colon tumor (937204)
LIB_ID: 268
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: T84 carcinoma cell line
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. From carcinoma cell line T84. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene colon tumor (937221)

NAME: Stratagene colon tumor (937221)
LIB_ID: 275
ORGANISM: human
STAGE: adult
ORGAN: colon
TISSUE: HT-29 (HTB 38) adenocarcinoma, moderately differentiated grade II
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. From adenocarcinoma HT-29 (HTB 38), moderately differentiated grade II. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

NCI_CGAP_Sar4

NAME: NCI_CGAP_Sar4
LIB_ID: 1357
ORGANISM: human
ORGAN: connective tissue
TISSUE: pool of 5 tumors: myxoid liposarcoma, solitary fibrous tumor (sarcoma), malignant fibrous histiocytoma, gastrointestinal stromal tumor, mesothelioma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.6 kb. Library constructed by Life Technologies, catalog #12004-016. Note: this is a NCI_CGAP Library.
||

NIA Human H1 Embryonic Stem Cells cDNA Library (Long)

NAME: NIA Human H1 Embryonic Stem Cells cDNA Library (Long)
LIB_ID: 2045
ORGANISM: human
SEX: unknown
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: undifferentiated ES cell line H1
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
' This is a long-transcript enriched cDNA library (Ref. Genome Res. 11: 1553-1558 (2001). [PMID: 11544199]). Undifferentiated human ES cell line H1 was obtained from WiCell Research Institute, Inc., Madison, Wi, cultured according to their instructions, and prepared for RNA extraction by Drs. Drs. Irene Ginis and Mahendra S. Rao. Briefly, cells were cultured on mouse embryonic fibroblast (MEF) feeders in ES cell medium consisting of DMEM/F12 (Invitrogen/GIBCO 11330-032) supplemented with 20% Knockout Serum Replacement, 100 mM MEM nonessential amino acids, 0.55 mM beta-mercaptoethanol, 2 mM L-glutamine, antibiotics/antimycotics, and with 4 ng/ml human basic fibroblast growth factor (bFGF) (all from Invitrogen/GIBCO). MEF derived from E12.5 mouse embryo were purchased from StemCell Technologiea, Inc. Vancouver, Canada. MEF were expanded on 0.5% bovine gelatin-coated dishes in DMEM medium (cat#11965-092) supplemented with 10% FBS (heat inactivated, Hyclone cat#3007103), 2 mM glutamine and 100 mM MEM non-essential amino acids. Subconfluent MEF cultures were treated with 1 mg/ml Mitomycin C (Sigma) for 3 hours to arrest cell division, trypsinized and plated at 0.2x105/cm2 onto 5% bovine collagen-coated dishes overnight. Feeders were washed twice with PBS; and then incubated in ES cell medium for at least 1 hour before plating ES cells. MEF''s of passage 2-3 were used as feeders. ES cells plated on top of MEF feeders were cultured at 5% CO2/5% O2 humidified tissue culture incubator from Thermo Forma. They formed round colonies with defined edges and were positive for alkaline phosphatase and SSEA-4. When confluent (18-10 days after plating) ES cells were treated with 1 mg/ml collagenase, type IV (Invitrogen/GIBCO) for 5-10 min and gently scraped off with 5 ml pipette. Cells from 4X6cm dishes were spun at 500 rpm for 3 min and the pellet was used for RNA purification. RNA was purified with TRIzol Reagent from Invitrogen. Double-stranded cDNAs were synthesized with an Oligo(dT) primer [Invitrogen: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3''] from 3.45g of total RNA, treated with T4 DNA polymerase, and purified by ethanol- precipitation. The cDNAs were ligated to Lone-linker LL-Sal4, purified by phenol/chloroform extraction, and separated from free linkers by Centricon-100 column. Then, the cDNAs were amplified by long-range high fidelity PCR using Ex Taq polymerase (Takara) with a primer Sal4-S. The products were purified by phenol/chloroform extraction and Centricon-100 column. The cDNAs were digested with SalI and NotI enzymes and cloned into SalI/NotI site of pCMV- SPORT6 plasmid vector. The DH10B E. coli host was transformed with the ligation mixture by the standard chemical method. The average insert size is about 3.6kb. The library was constructed by Yulan Piao. Note: this is a NIH_MGC Library. '
||

NIH_MGC_193

NAME: NIH_MGC_193
LIB_ID: 2046
ORGANISM: human
SEX: unknown
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic Stem Cells HI
HOST: DH10B TonA
VECTOR: pCI-neo (updated)
V_TYPE: plasmid
RE_5': AscI
RE_3': PacI
DESCR:
' Constructed by Gina Zastrow using the Bradfield Laboratory RACE method (University of Wisconsin). Note: this is a NIH_MGC Library. '
||

NIH_MGC_200

NAME: NIH_MGC_200
LIB_ID: 2113
ORGANISM: human
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: ES cell line BG01, undifferentiated
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCCT(30)-3' and directionally cloned using 5' adaptor sequence: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCrGrGrG-3' Average insert size 0.96 kb (range 0.4-2.5 kb). Library is normalized and enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Reference: Biotechniques 30:892-897 (2001). Note: this is a NIH_MGC Library.
||

NIH_MGC_201

NAME: NIH_MGC_201
LIB_ID: 2114
ORGANISM: human
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: ES cell line BG01, derived early primative ectodermal like (EPL) cells, the in vitro equivalent of the next pluripotent stage of development
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCCT(30)-3' and directionally cloned using 5' adaptor sequence: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCrGrGrG-3' Average insert size 1.04 kb (range 0.5-2.5 kb). Library is normalized and enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Reference: Biotechniques 30:892-897 (2001). Note: this is a NIH_MGC Library.
||

NIH_MGC_202

NAME: NIH_MGC_202
LIB_ID: 2115
ORGANISM: human
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: ES cell line BG01, derived embyoid bodies with high levels of neural progenitors
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCGAGGCGGCCT(30)-3' and directionally cloned using 5' adaptor sequence: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTATGGCCrGrGrG-3' Average insert size 0.9 kb (range 0.4-2.5 kb). Library is normalized and enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Reference: Biotechniques 30:892-897 (2001). Note: this is a NIH_MGC Library.
||

NIH_MGC_239

NAME: NIH_MGC_239
LIB_ID: 2095
ORGANISM: human
SEX: unknown
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic stem cell, pooled H7 ES cell line, passage 22
HOST: DH10B TonA
VECTOR: pCI-neo (updated)
V_TYPE: plasmid
RE_5': AscI
RE_3': PacI
DESCR:
Bradfield Lab RACE method. Size selection 500-2kb, average insert size 550bp
||

NIH_MGC_240

NAME: NIH_MGC_240
LIB_ID: 2096
ORGANISM: human
SEX: unknown
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic stem cells pooled from H7 ES cell line, passage 22
HOST: DH10B TonA
VECTOR: pCI-neo (updated)
V_TYPE: plasmid
RE_5': AscI
RE_3': PacI
DESCR:
Bradfield Lab RACE method, Size selection 2kb-5kb, average insert size 550bp.
||

NIH_MGC_258

NAME: NIH_MGC_258
LIB_ID: 2185
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos and differentiated to an early endodermal cell type. Markers: GATA4+, MixL1+, Msx1+, HNF4alpha+, AFP-, Passage number: 40
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
||

NIH_MGC_259

NAME: NIH_MGC_259
LIB_ID: 2186
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos and differentiated to an early endodermal cell type. Markers: GATA4+, MixL1+, Msx1+, HNF4alpha+, AFP-, Passage number: 40
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
'cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.2 kb resulted in an average insert size of 1.6 kb. This primary library is normalized to Cot10 (non-normalized primary library is NIH_MGC_258) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.'
||

NIH_MGC_260

NAME: NIH_MGC_260
LIB_ID: 2187
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos. Markers: SSEA1-, SSEA3+, SSEA4+, Tra 1-60+, Tra 1-81+, CD9+, Alk Phos+, Oct4+, Nanog+. Passage number: 21
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
'cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.9 kb. This primary library is not normalized (normalized primary library is NIH_MGC_261) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.'
||

NIH_MGC_261

NAME: NIH_MGC_261
LIB_ID: 2188
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic stem cells isolated from the inner cell mass of blastocyst stage embryos. Markers: SSEA1-, SSEA3+, SSEA4+, Tra 1-60+, Tra 1-81+, CD9+, Alk Phos+, Oct4+, Nanog+. Passage number: 21
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
'cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.5 kb. This primary library is normalized to Cot10 (non-normalized primary library is NIH_MGC_260) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.'
||

NIH_MGC_262

NAME: NIH_MGC_262
LIB_ID: 2189
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic Stem cells isolated from the inner cell mass of blastocyst stage embryos and differentiated to an early neural progenitor cell type. Markers: Nestin+, Musashi+-. Passage number: 18
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
'cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 2.1 kb. This primary library is not normalized (normalized primary library is NIH_MGC_263) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.'
||

NIH_MGC_263

NAME: NIH_MGC_263
LIB_ID: 2190
ORGANISM: human
SEX: male
STAGE: embryo
ORGAN: embryonic stem cell
TISSUE: Embryonic Stem cells isolated from the inner cell mass of blastocyst stage embryos and differentiated to an early neural progenitor cell type. Markers: Nestin+, Musashi+-. Passage number: 18
HOST: DH10B TonA
VECTOR: pExpress-1
V_TYPE: plasmid
RE_5': EcoRV
RE_3': NotI
DESCR:
'cDNA was primed using oligo-dT primer: 5''-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3'' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1.25 kb resulted in an average insert size of 1.6 kb. This primary library is normalized to Cot10 (non-normalized primary library is NIH_MGC_262) and was constructed by Express Genomics (Frederick, MD). Note: this is a NIH_MGC library.'
||

Stratagene endothelial cell (937223)

NAME: Stratagene endothelial cell (937223)
LIB_ID: 277
ORGANISM: human
STAGE: infant
ORGAN: endothelial cell
TISSUE: umbilical vein endothelial cells, 1 passage
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Umbilical vein endothelial cells, passaged once. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

NCI_CGAP_Eso2

NAME: NCI_CGAP_Eso2
LIB_ID: 1243
ORGANISM: human
ORGAN: esophagus
TISSUE: squamous cell carcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Life Technologies catalog #: 11502-010 Note: this is a NCI_CGAP Library.
||

NIH_MGC_16

NAME: NIH_MGC_16
LIB_ID: 1475
ORGANISM: human
ORGAN: eye
TISSUE: retinoblastoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_43

NAME: NIH_MGC_43
LIB_ID: 1679
ORGANISM: human
ORGAN: eye
TISSUE: normal pigmented retinal epithelium
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_67

NAME: NIH_MGC_67
LIB_ID: 1616
ORGANISM: human
ORGAN: eye
TISSUE: retinoblastoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.75 kb. Library constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

Soares N2b4HR

NAME: Soares N2b4HR
LIB_ID: 85
ORGANISM: human
STAGE: adult
ORGAN: eye
TISSUE: retina
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCACTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot50, and has an average insert length of 2 kb. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
||

Soares N2b5HR

NAME: Soares N2b5HR
LIB_ID: 86
ORGANISM: human
STAGE: adult
ORGAN: eye
TISSUE: retina
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCACTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot50, and has an average insert length of 1 kb. Library constructed by Bento Soares and M. Fatima Bonaldo (Columbia University).
||

Stratagene corneal stroma (937222)

NAME: Stratagene corneal stroma (937222)
LIB_ID: 276
ORGANISM: human
STAGE: adult
AGE: 76
ORGAN: eye
TISSUE: corneal stroma grown out of explants
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Corneal fibroblasts grown out of explants from 76 year old donor. Average insert size: 1.5 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene fetal retina (937202)

NAME: Stratagene fetal retina (937202)
LIB_ID: 267
ORGANISM: human
STAGE: fetal
ORGAN: eye
TISSUE: pooled retinal tissue
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pooled retinal tissue. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Soares Nb2HF8-9w

NAME: Soares Nb2HF8-9w
LIB_ID: 385
ORGANISM: human
STAGE: fetal
ORGAN: fetus
TISSUE: total fetus
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from mRNA obtained from pooled 8-9 week (total) fetus material with a Not I - oligo(dT) primer [5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCTTAATTTTTTTTTTTTTTTTT-3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares NbHSF

NAME: Soares NbHSF
LIB_ID: 219
ORGANISM: human
STAGE: adult
ORGAN: fibroblast
TISSUE: senescent cells
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAACCATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
||

Stratagene fibroblast (937212)

NAME: Stratagene fibroblast (937212)
LIB_ID: 271
ORGANISM: human
STAGE: adult
ORGAN: fibroblast
TISSUE: WI-38 lung fibroblast cell line, grown to confluency
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. WI-38 lung fibroblast cell line grown to confluency. Average insert size: 0.8 kb; Uni-ZAP XR Vector. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

NCI_CGAP_GU1

NAME: NCI_CGAP_GU1
LIB_ID: 1352
ORGANISM: human
ORGAN: genitourinary
TISSUE: high-grade transitional cell carcinoma, 2 pooled
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GC1

NAME: NCI_CGAP_GC1
LIB_ID: 404
ORGANISM: human
STAGE: adult
ORGAN: germ cell
TISSUE: bulk seminoma
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GC2

NAME: NCI_CGAP_GC2
LIB_ID: 429
ORGANISM: human
STAGE: adult
ORGAN: germ cell
TISSUE: bulk tumor
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Bulk germ cell tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GC3

NAME: NCI_CGAP_GC3
LIB_ID: 633
ORGANISM: human
STAGE: adult
ORGAN: germ cell
TISSUE: 3 pooled tumors, including broad spectrum tumor types
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAATCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is not normalized. Normalized version of this library is named NCI_CGAP_GC4. Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GC4

NAME: NCI_CGAP_GC4
LIB_ID: 634
ORGANISM: human
STAGE: adult
ORGAN: germ cell
TISSUE: 3 pooled tumors, including broad spectrum tumor types
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from 3 pooled germ cell tumors, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAATCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized. Non-normalized version of this library is named NCI_CGAP_GC3. Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GC5

NAME: NCI_CGAP_GC5
LIB_ID: 533
ORGANISM: human
STAGE: adult
ORGAN: germ cell
TISSUE: pooled tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Mixed germ cell tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.7 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_GC6

NAME: NCI_CGAP_GC6
LIB_ID: 1234
ORGANISM: human
STAGE: adult
ORGAN: germ cell
TISSUE: 3 pooled tumors, including broad spectrum tumor types
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Plasmid DNA from the normalized library NCI_CGAP_GC4 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1257096-1258631, 1469064-1470983, and 1475592-1476743). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN5

NAME: NCI_CGAP_HN5
LIB_ID: 1328
ORGANISM: human
SEX: unknown
ORGAN: gingiva
TISSUE: normal gingiva -- cell line from primary keratinocytes
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' AATTCGGCACGAG 3' 3' GCCGTGCTC 5' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' EcoRI site appears to have been lost in a fraction of the clones. Library constructed by Stratagene; available through Mary May, PhD (Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH; mmay@yoda.nidr.nih.gov). Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN6

NAME: NCI_CGAP_HN6
LIB_ID: 1329
ORGANISM: human
SEX: unknown
ORGAN: gingiva
TISSUE: normal gingiva -- cell line of immortalized keratinocytes
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' AATTCGGCACGAG 3' 3' GCCGTGCTC 5' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' EcoRI site appears to have been lost in a fraction of the clones. Library constructed by Stratagene; available through Mary May, PhD (Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH; mmay@yoda.nidr.nih.gov). Note: this is a NCI_CGAP Library.
||

NIH_MGC_182

NAME: NIH_MGC_182
LIB_ID: 2027
ORGANISM: human
ORGAN: gut
TISSUE: pooled gut (stomach, small intestine, colon, esophagus, gall bladder)
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.68. Library was constructed by Invitrogen. Note: this is a NIH_MGC Library. '
||

COGENE 4PA1

NAME: COGENE 4PA1
LIB_ID: 1820
ORGANISM: human
SEX: unknown
STAGE: embryo
ORGAN: head/neck
TISSUE: 1st pharyngeal arch, pooled samples
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-1.2 kb. Normalized to Cot10. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

COGENE 6E MAN

NAME: COGENE 6E MAN
LIB_ID: 1876
ORGANISM: human
STAGE: embryo
ORGAN: head/neck
TISSUE: mandible
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-2.0 kb. Normalized to Cot5. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

COGENE 6E MAX

NAME: COGENE 6E MAX
LIB_ID: 1875
ORGANISM: human
STAGE: embryo
ORGAN: head/neck
TISSUE: maxilla
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-1.8 kb. Normalized to Cot5. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

COGENE 8.5 EAT

NAME: COGENE 8.5 EAT
LIB_ID: 1877
ORGANISM: human
STAGE: embryo
ORGAN: head/neck
TISSUE: anterior tongue
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-1.6 kb. Normalized to Cot5. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

COGENE 8.5 EPT

NAME: COGENE 8.5 EPT
LIB_ID: 1819
ORGANISM: human
SEX: unknown
STAGE: embryo
ORGAN: head/neck
TISSUE: posterior tongue
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA primed using oligo-dT primer, directionally cloned into UDG sites of pAMP1. Size selected for insert sizes ranging from 0.2-2.5 kb. Normalized to Cot10. Primary library, non-amplified. Library constructed by M. Lovett. For more information on this library, please contact R. Tidwell (Washington University) or visit the COGENE website at http://hg.wustl.edu/COGENE/.
||

NCI_CGAP_HN20

NAME: NCI_CGAP_HN20
LIB_ID: 1604
ORGANISM: human
ORGAN: head/neck
TISSUE: normal
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from head/neck tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN21

NAME: NCI_CGAP_HN21
LIB_ID: 1605
ORGANISM: human
ORGAN: head/neck
TISSUE: nasopharyngeal carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from head/neck tumor, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 300 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIH_MGC_102

NAME: NIH_MGC_102
LIB_ID: 1767
ORGANISM: human
ORGAN: head/neck
TISSUE: salivary gland, epidermoid carcinoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

Barstead HPL-RB3

NAME: Barstead HPL-RB3
LIB_ID: 1059
ORGANISM: human
SEX: male
STAGE: adult
AGE: 64
ORGAN: heart
TISSUE: aorta
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [5' AATTCGGATCCAAC 3' and 5' GTTGGATCCG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

Barstead HPL-RB6

NAME: Barstead HPL-RB6
LIB_ID: 1310
ORGANISM: human
SEX: male
STAGE: adult
AGE: 64
ORGAN: heart
TISSUE: aorta
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [5' AATTCGGATCGAAC 3' and 5' GTTGGATCGG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

Clontech heart (HL3026s)

NAME: Clontech heart (HL3026s)
LIB_ID: 70
ORGANISM: human
STAGE: adult
ORGAN: heart
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

Life Tech heart (10419-018)

NAME: Life Tech heart (10419-018)
LIB_ID: 112
ORGANISM: human
SEX: female
STAGE: adult
AGE: 50
ORGAN: heart
HOST: DH12S
VECTOR: pCMV-SPORT
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
directionally cloned, average insert size 1.72 kb
||

NIH_MGC_74

NAME: NIH_MGC_74
LIB_ID: 1642
ORGANISM: human
ORGAN: heart
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.25 kb (range 0.6-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

Soares NbHH19W

NAME: Soares NbHH19W
LIB_ID: 227
ORGANISM: human
STAGE: fetal
ORGAN: heart
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATCTTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
||

Clontech kidney (HL3001s)

NAME: Clontech kidney (HL3001s)
LIB_ID: 66
ORGANISM: human
STAGE: adult
ORGAN: kidney
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

Gessler Wilms' tumor

NAME: Gessler Wilms' tumor
LIB_ID: 440
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: six pooled nephroblastomas; Wilms' tumor
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
RNA was prepared from a pool of anonymous Wilms' tumors by acid-phenol extraction. cDNA was synthesized with the Life Technologies Superscript plasmid system using an oligo dT-NotI primer for first strand synthesis. A SalI adaptor was ligated to the 5' ends of the clones. cDNA was size- selected on columns, NotI digested, and ligated into NotI/SalI-cut pSPORT1 and electroporated into DH10B cells. Average insert size: 2.0 kb. Library was constructed by Dr. Manfred Gessler.
||

Life Tech kidney (10420-016)

NAME: Life Tech kidney (10420-016)
LIB_ID: 106
ORGANISM: human
SEX: male
STAGE: adult
AGE: 38
ORGAN: kidney
HOST: DH12S
VECTOR: pCMV-SPORT
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Caucasian. Average insert size: 1.32 kb; pCMV-SPORT vector.
||

NCI_CGAP_Kid1

NAME: NCI_CGAP_Kid1
LIB_ID: 471
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: papillary renal cell carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive kidney tumor, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid11

NAME: NCI_CGAP_Kid11
LIB_ID: 1235
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: 2 pooled
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Plasmid DNA from the normalized library NCI_CGAP_Kid3 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1322376-1323911, 1456007-1456775, and 1500552-1502855). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid12

NAME: NCI_CGAP_Kid12
LIB_ID: 1309
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: 2 pooled tumors, clear cell type
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Plasmid DNA from the normalized library NCI_CGAP_Kid5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1323912-1325831, 1471368-1472903 and 1492104-1493255). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid13

NAME: NCI_CGAP_Kid13
LIB_ID: 1351
ORGANISM: human
ORGAN: kidney
TISSUE: primary Wilms' tumor, Wilms' tumor metastatic to brain, pooled
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid3

NAME: NCI_CGAP_Kid3
LIB_ID: 687
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: 2 pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' AACTGGAAGAATTCGCGGCCGCAATGCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to Eco RI adaptors (Pharmacia), digested with Not I and cloned into the Not I and Eco RI sites of the modified pT7T3 vector. mRNA source: 2 pooled kidneys. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid5

NAME: NCI_CGAP_Kid5
LIB_ID: 688
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: 2 pooled tumors, clear cell type
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' AACTGGAAGAATTCGCGGCCGCAATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid6

NAME: NCI_CGAP_Kid6
LIB_ID: 545
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: pooled tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pooled kidney tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.0 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid7

NAME: NCI_CGAP_Kid7
LIB_ID: 610
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: 5 pooled tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. 5 pooled kidney tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.3 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Kid8

NAME: NCI_CGAP_Kid8
LIB_ID: 1241
ORGANISM: human
STAGE: adult
ORGAN: kidney
TISSUE: renal cell cancer
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Life Technologies catalog #: 11524-014 Note: this is a NCI_CGAP Library.
||

NIH_MGC_108

NAME: NIH_MGC_108
LIB_ID: 1813
ORGANISM: human
ORGAN: kidney
TISSUE: Wilms' tumor
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_14

NAME: NIH_MGC_14
LIB_ID: 1427
ORGANISM: human
ORGAN: kidney
TISSUE: renal cell adenocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_45

NAME: NIH_MGC_45
LIB_ID: 1680
ORGANISM: human
ORGAN: kidney
TISSUE: renal carcinoma (ascites)
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_58

NAME: NIH_MGC_58
LIB_ID: 1586
ORGANISM: human
ORGAN: kidney
TISSUE: hypernephroma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.35 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_75

NAME: NIH_MGC_75
LIB_ID: 1656
ORGANISM: human
ORGAN: kidney
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_89

NAME: NIH_MGC_89
LIB_ID: 1725
ORGANISM: human
ORGAN: kidney
TISSUE: hypernephroma, cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.3 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NCI_CGAP_HN2

NAME: NCI_CGAP_HN2
LIB_ID: 763
ORGANISM: human
SEX: unknown
ORGAN: larynx
TISSUE: squamous cell carcinoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' Library constructed by Stratagene. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lar1

NAME: NCI_CGAP_Lar1
LIB_ID: 546
ORGANISM: human
STAGE: adult
ORGAN: larynx
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Larynx squamous carcinoma. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb. Note: this is a NCI_CGAP Library.
||

Life Tech leukocyte (10421-014)

NAME: Life Tech leukocyte (10421-014)
LIB_ID: 107
ORGANISM: human
STAGE: adult
ORGAN: leukocyte
TISSUE: pooled
HOST: DH12S
VECTOR: pCMV-SPORT
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size: 1.3 kb; pCMV-SPORT vector.
||

NIH_MGC_118

NAME: NIH_MGC_118
LIB_ID: 1787
ORGANISM: human
STAGE: adult
ORGAN: leukocyte
TISSUE: leukocytes from non-activated multiple adult donors
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source leukocytes from anonymous pool of non-activated adult donors. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7 kb, insert size range 1.2-3.3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 027. Note: this is a NIH_MGC Library. '
||

Clontech fetal liver (HL3020s)

NAME: Clontech fetal liver (HL3020s)
LIB_ID: 72
ORGANISM: human
STAGE: fetal
ORGAN: liver
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

Clontech liver (HL3006s)

NAME: Clontech liver (HL3006s)
LIB_ID: 67
ORGANISM: human
STAGE: adult
ORGAN: liver
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
directionally cloned
||

Life Tech liver (10422-012)

NAME: Life Tech liver (10422-012)
LIB_ID: 113
ORGANISM: human
SEX: female
STAGE: juvenile
AGE: 9
ORGAN: liver
HOST: DH12S
VECTOR: pCMV-SPORT
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
directionally cloned, average insert size 1.6 kb
||

NCI_CGAP_Gas2

NAME: NCI_CGAP_Gas2
LIB_ID: 735
ORGANISM: human
ORGAN: liver
TISSUE: gastric carcinoma metastatic to liver
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Life Technologies catalog #: 10989-010 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Li1

NAME: NCI_CGAP_Li1
LIB_ID: 476
ORGANISM: human
STAGE: adult
ORGAN: liver
TISSUE: hepatocytes
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal liver hepatocytes, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Li2

NAME: NCI_CGAP_Li2
LIB_ID: 469
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: liver
TISSUE: hepatocellular carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive hepatocellular carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size- selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Li5

NAME: NCI_CGAP_Li5
LIB_ID: 733
ORGANISM: human
ORGAN: liver
TISSUE: hepatic adenoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.8 kb. Life Technologies catalog #: 10986-016 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Li8

NAME: NCI_CGAP_Li8
LIB_ID: 1405
ORGANISM: human
ORGAN: liver
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr20

NAME: NCI_CGAP_Pr20
LIB_ID: 494
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: liver
TISSUE: prostate tumor metastasis to liver
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from metastatic prostate lesion of the liver, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIH_MGC_100

NAME: NIH_MGC_100
LIB_ID: 1774
ORGANISM: human
ORGAN: liver
TISSUE: hepatocellular carcinoma, cell line
HOST: DH10B TonA
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_290

NAME: NIH_MGC_290
LIB_ID: 2262
ORGANISM: human
ORGAN: liver
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_291 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_291

NAME: NIH_MGC_291
LIB_ID: 2263
ORGANISM: human
ORGAN: liver
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_290 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_292

NAME: NIH_MGC_292
LIB_ID: 2284
ORGANISM: human
ORGAN: liver
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_293 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_293

NAME: NIH_MGC_293
LIB_ID: 2285
ORGANISM: human
ORGAN: liver
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_292 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_76

NAME: NIH_MGC_76
LIB_ID: 1643
ORGANISM: human
ORGAN: liver
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.85 kb (range 1.0-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_90

NAME: NIH_MGC_90
LIB_ID: 1726
ORGANISM: human
ORGAN: liver
TISSUE: adenocarcinoma, cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.7 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

Stratagene liver (937224)

NAME: Stratagene liver (937224)
LIB_ID: 63
ORGANISM: human
SEX: male
STAGE: adult
AGE: 49
ORGAN: liver
TISSUE: hepatectomy
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Hepatectomy from normal male caucasian. Average insert size: 1.1 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Clontech fetal lung (HL3022s)

NAME: Clontech fetal lung (HL3022s)
LIB_ID: 68
ORGANISM: human
STAGE: fetal
ORGAN: lung
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
directionally cloned
||

Clontech lung (HL3004s)

NAME: Clontech lung (HL3004s)
LIB_ID: 71
ORGANISM: human
STAGE: adult
ORGAN: lung
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

Life Tech lung (10424-018)

NAME: Life Tech lung (10424-018)
LIB_ID: 109
ORGANISM: human
SEX: male
STAGE: adult
AGE: 17
ORGAN: lung
HOST: DH12S
VECTOR: pCMV-SPORT
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
directionally cloned, average insert size 1.3 kb
||

NCI_CGAP_DH0

NAME: NCI_CGAP_DH0
LIB_ID: 1915
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: metastatic chondrosarcoma, cell line VS-8
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGATCATTGCTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_DH1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_DH1

NAME: NCI_CGAP_DH1
LIB_ID: 1916
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: metastatic chondrosarcoma, cell line VS-8
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAGATCATTGCTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (non-normalized version of the library is NCI_CGAP_DH0), and was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_DI0

NAME: NCI_CGAP_DI0
LIB_ID: 1917
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: focal fibrosis, pool
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCATACGCGGTCTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_DT0

NAME: NCI_CGAP_DT0
LIB_ID: 1918
ORGANISM: human
STAGE: adult
AGE: 1
ORGAN: lung
TISSUE: metastatic chondrosarcoma
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAACTGTTCGGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version of this library is also available (NCI_CGAP_DT1). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_DT1

NAME: NCI_CGAP_DT1
LIB_ID: 1919
ORGANISM: human
STAGE: adult
AGE: 1
ORGAN: lung
TISSUE: metastatic chondrosarcoma
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAACTGTTCGGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3'(Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (non-normalized version of the library is NCI_CGAP_DT0), and was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_Lu1

NAME: NCI_CGAP_Lu1
LIB_ID: 427
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: bulk tumor
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Bulk lung tumor. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu13

NAME: NCI_CGAP_Lu13
LIB_ID: 1339
ORGANISM: human
ORGAN: lung
TISSUE: adenocarcinoma, poorly differentiated
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCCGGTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu19

NAME: NCI_CGAP_Lu19
LIB_ID: 1313
ORGANISM: human
SEX: unknown
ORGAN: lung
TISSUE: squamous cell carcinoma, poorly differentiated, 4 pooled tumors including primary and metastatic
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from pooled lung tumor tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGACAGCTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu21

NAME: NCI_CGAP_Lu21
LIB_ID: 1404
ORGANISM: human
ORGAN: lung
TISSUE: small cell carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu24

NAME: NCI_CGAP_Lu24
LIB_ID: 1312
ORGANISM: human
ORGAN: lung
TISSUE: neuroendocrine lung carcinoid
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Plasmid DNA from the normalized library NCI_CGAP_Lu5 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 1414920-1417991 and 1520904-1522439). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu25

NAME: NCI_CGAP_Lu25
LIB_ID: 1307
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: bronchioalveloar carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from lung carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu26

NAME: NCI_CGAP_Lu26
LIB_ID: 1308
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: invasive carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from lung adenocarcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu27

NAME: NCI_CGAP_Lu27
LIB_ID: 1354
ORGANISM: human
ORGAN: lung
TISSUE: poorly-differentiated adenocarcinoma, 4 pooled
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies, catalog #12007-019. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu28

NAME: NCI_CGAP_Lu28
LIB_ID: 1353
ORGANISM: human
ORGAN: lung
TISSUE: squamous cell carcinoma, 2 pooled
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies, catalog #12008-017. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu31

NAME: NCI_CGAP_Lu31
LIB_ID: 1375
ORGANISM: human
SEX: male
STAGE: fetal
ORGAN: lung
TISSUE: lung, cell line
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Cloned unidirectionally, no 5' adaptor. Primer: Oligo dT. Full-length library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu34

NAME: NCI_CGAP_Lu34
LIB_ID: 1392
ORGANISM: human
ORGAN: lung
TISSUE: large cell carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu34.1

NAME: NCI_CGAP_Lu34.1
LIB_ID: 1402
ORGANISM: human
ORGAN: lung
TISSUE: large cell carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu5

NAME: NCI_CGAP_Lu5
LIB_ID: 722
ORGANISM: human
ORGAN: lung
TISSUE: neuroendocrine lung carcinoid
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from neuroendocrine lung carcinoid, and was then primed with a Not I - oligo(dT) primer [5'-AACTGGAAGAATTCGCGGCCGCCAACTTTTTTTTTTTTTTTTTTT-3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized and was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu6

NAME: NCI_CGAP_Lu6
LIB_ID: 736
ORGANISM: human
ORGAN: lung
TISSUE: small cell carcinoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. Life Technologies catalog #: 10991-016 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lu7

NAME: NCI_CGAP_Lu7
LIB_ID: 737
ORGANISM: human
ORGAN: lung
TISSUE: adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Life Technologies catalog #: 10992-014 Note: this is a NCI_CGAP Library.
||

NIH_MGC_101

NAME: NIH_MGC_101
LIB_ID: 1871
ORGANISM: human
ORGAN: lung
TISSUE: epidermoid carcinoma, cell line
HOST: DH10B TonA
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_18

NAME: NIH_MGC_18
LIB_ID: 1651
ORGANISM: human
ORGAN: lung
TISSUE: large cell carcinoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_212

NAME: NIH_MGC_212
LIB_ID: 2105
ORGANISM: human
SEX: unknown
ORGAN: lung
TISSUE: pool of 3 metastases
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled lung metastases (cell lines) . The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCGATAAGGCCATTTTTTTTTTTTTTTTTT -3') contains the sequence tag GATAAGGCCA between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library constructed by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa). Note: this is a NIH_MGC Library.
||

NIH_MGC_213

NAME: NIH_MGC_213
LIB_ID: 2106
ORGANISM: human
SEX: unknown
ORGAN: lung
TISSUE: pool of 3 metastases
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled lung metastases (cell lines) . The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCGATAAGGCCATTTTTTTTTTTTTTTTTT -3') contains the sequence tag GATAAGGCCA between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library constructed by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa). Note: this is a NIH_MGC Library.
||

NIH_MGC_214

NAME: NIH_MGC_214
LIB_ID: 2107
ORGANISM: human
SEX: unknown
ORGAN: lung
TISSUE: pool of 3 metastases
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled lung metastases (cell lines) . The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCGATAAGGCCATTTTTTTTTTTTTTTTTT -3') contains the sequence tag GATAAGGCCA between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library constructed by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa). Note: this is a NIH_MGC Library.
||

NIH_MGC_215

NAME: NIH_MGC_215
LIB_ID: 2108
ORGANISM: human
SEX: unknown
ORGAN: lung
TISSUE: pool of 3 metastases
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Non-normalized full-length enriched library from pooled lung metastases (cell lines) . The library was constructed in the pYX-Asc vector according to the procedure described in Genome Research 6:791-806. The oliogonucleotide used to prime first-strand synthesis (5'-TGTTACCATTCTGATGTTGGAGCGGCCGCGATAAGGCCATTTTTTTTTTTTTTTTTT -3') contains the sequence tag GATAAGGCCA between the NotI cloning site and dT(18) stretch. Cloning was accomplished via 5' and 3' adaptors as follows: 5'-GAATTCGGCACGAGG-3' and 5'-GCGGCCGCCAGCCACGAC-3'. Library constructed by Maria de Fatima Bonaldo and M. Bento Soares (University of Iowa). Note: this is a NIH_MGC Library.
||

NIH_MGC_265

NAME: NIH_MGC_265
LIB_ID: 2171
ORGANISM: human
SEX: female
STAGE: adult
AGE: 61
ORGAN: lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_273 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_273

NAME: NIH_MGC_273
LIB_ID: 2205
ORGANISM: human
SEX: female
STAGE: adult
AGE: 61
ORGAN: lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_265 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_298

NAME: NIH_MGC_298
LIB_ID: 2266
ORGANISM: human
ORGAN: lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_299 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_299

NAME: NIH_MGC_299
LIB_ID: 2267
ORGANISM: human
ORGAN: lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_298 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_300

NAME: NIH_MGC_300
LIB_ID: 2288
ORGANISM: human
ORGAN: lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_301 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_301

NAME: NIH_MGC_301
LIB_ID: 2289
ORGANISM: human
ORGAN: lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_300 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_59

NAME: NIH_MGC_59
LIB_ID: 1587
ORGANISM: human
ORGAN: lung
TISSUE: mucoepidermoid carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_68

NAME: NIH_MGC_68
LIB_ID: 1617
ORGANISM: human
ORGAN: lung
TISSUE: large cell carcinoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_69

NAME: NIH_MGC_69
LIB_ID: 1618
ORGANISM: human
ORGAN: lung
TISSUE: large cell carcinoma, undifferentiated, cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_7

NAME: NIH_MGC_7
LIB_ID: 1408
ORGANISM: human
ORGAN: lung
TISSUE: small cell carcinoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_77

NAME: NIH_MGC_77
LIB_ID: 1657
ORGANISM: human
ORGAN: lung
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.9 kb (range 0.5-4.0 kb). 12/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

Soares NbHL19W

NAME: Soares NbHL19W
LIB_ID: 216
ORGANISM: human
STAGE: fetal
ORGAN: lung
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCAATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
||

Stratagene lung (937210)

NAME: Stratagene lung (937210)
LIB_ID: 60
ORGANISM: human
SEX: male
STAGE: adult
AGE: 72
ORGAN: lung
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Normal lung. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene lung carcinoma (937218)

NAME: Stratagene lung carcinoma (937218)
LIB_ID: 273
ORGANISM: human
STAGE: adult
ORGAN: lung
TISSUE: small cell carcinoma cell line NCI-H69
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Small cell carcinoma cell line NCI-H69. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

NIH_MGC_3

NAME: NIH_MGC_3
LIB_ID: 1419
ORGANISM: human
ORGAN: lymph
TISSUE: lymph from Burkitt lymphoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7a
V_TYPE: plasmid
RE_5': CeuI
RE_3': SceI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into CeuI/SceI sites using the following 5'' adaptor: taactataacggtcctaaggtagcga and 3'' adaptor: tttcattacctctttctccgcaccccacataaa. Average insert size 840 bp. Library prepared by Edge BioSystems. Note: this is a NIH_MGC Library. '
||

NIH_MGC_36

NAME: NIH_MGC_36
LIB_ID: 1502
ORGANISM: human
ORGAN: lymph
TISSUE: germinal center B-cells mRNA size selected 1.35-2.37 kb
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
' Library was derived from 1.35-2.37 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library. '
||

NIH_MGC_37

NAME: NIH_MGC_37
LIB_ID: 1503
ORGANISM: human
ORGAN: lymph
TISSUE: germinal center B cells mRNA size selected 0.5-1.35 kb
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
' Library was derived from 0.5-1.35 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library. '
||

NIH_MGC_38

NAME: NIH_MGC_38
LIB_ID: 1504
ORGANISM: human
ORGAN: lymph
TISSUE: germinal center B cells mRNA size selected 2.37-3.5 kb
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
' Library was derived from 2.37-3.5 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library. '
||

NIH_MGC_50

NAME: NIH_MGC_50
LIB_ID: 1507
ORGANISM: human
ORGAN: lymph
TISSUE: germinal center B-cells mRNA size selected 3.5-4.4 kb
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
' Library was derived from 3.5-4.4 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library. '
||

NIH_MGC_51

NAME: NIH_MGC_51
LIB_ID: 1508
ORGANISM: human
ORGAN: lymph
TISSUE: germinal center B-cells mRNA size selected 4.4-7.4 kb
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
' Library was derived from 4.4-7.4 kb mRNA size fraction;1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library. '
||

NIH_MGC_52

NAME: NIH_MGC_52
LIB_ID: 1509
ORGANISM: human
ORGAN: lymph
TISSUE: germinal center B-cells mRNA size selected 7.4-9.5 kb
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
' Library was derived from 7.4-9.5 kb mRNA size fraction; 1st strand cDNA was primed with a Not I - oligo(dT) primer: 5''-TGTTACCAATCTGAAGTGGGAGCGGCCGCATATGACTTTTTTTTTTTTTTTTTTTTTTTTT-3''; double-stranded cDNA was ligated to EcoRI adaptors 5''-AATTCGGCACGAGG-3'' and 5''-CCTCGTGCCG-3'' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M.Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library. '
||

NIH_MGC_63

NAME: NIH_MGC_63
LIB_ID: 1527
ORGANISM: human
SEX: male
STAGE: adult
AGE: 19
ORGAN: lymph
TISSUE: cell line, T lymphoblast, acute lymphoblastic leukemia
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
' Double-stranded cDNA was prepared from lymphocyte cell line. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CATAGGATCCGTCGACCCCCCCC-3'' and 3'' adaptor sequence: 5''-GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT-3''. This library was constructed using the CAP-trapper method for full-length enrichment and was constructed by Dr. Claudio Schneider (LNCIB-Area Science Park, Trieste, Italy). Note: this is a NIH_MGC Library. '
||

NIH_MGC_8

NAME: NIH_MGC_8
LIB_ID: 1425
ORGANISM: human
ORGAN: lymph
TISSUE: cell line, Burkitt lymphoma
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_85

NAME: NIH_MGC_85
LIB_ID: 1711
ORGANISM: human
ORGAN: lymph
TISSUE: lymphoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.867 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_99

NAME: NIH_MGC_99
LIB_ID: 1773
ORGANISM: human
ORGAN: lymph
TISSUE: lymph from lymphoma, cell line
HOST: DH10B TonA
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NCI_CGAP_HN1

NAME: NCI_CGAP_HN1
LIB_ID: 762
ORGANISM: human
ORGAN: lymph node
TISSUE: metastatic squamous cell carcinoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.3 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' Library constructed by Stratagene. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HSC2

NAME: NCI_CGAP_HSC2
LIB_ID: 1297
ORGANISM: human
ORGAN: lymph node
TISSUE: stem cell 34+/38+
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from bone marrow, stem cells 34+/38+, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lym12

NAME: NCI_CGAP_Lym12
LIB_ID: 1274
ORGANISM: human
ORGAN: lymph node
TISSUE: follicular mixed small and large cell
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.25 kb. Life Technologies catalog #: 11547-015 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lym3

NAME: NCI_CGAP_Lym3
LIB_ID: 594
ORGANISM: human
STAGE: adult
ORGAN: lymph node
TISSUE: pooled lymphomas (10) including a broad spectrum of tumor types
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. 10 pooled lymphomas. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 0.9 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lym5

NAME: NCI_CGAP_Lym5
LIB_ID: 1246
ORGANISM: human
STAGE: adult
ORGAN: lymph node
TISSUE: follicular lymphoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.2 kb. Non-amplified library. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Lym6

NAME: NCI_CGAP_Lym6
LIB_ID: 1247
ORGANISM: human
STAGE: adult
ORGAN: lymph node
TISSUE: mantle cell lymphoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.8 kb. Non-amplified library. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

NCI_CGAP_FT0

NAME: NCI_CGAP_FT0
LIB_ID: 2086
ORGANISM: human
ORGAN: macrophage
TISSUE: alveolar macrophage, pool of 81, challenged with different treatments
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGGCCATGCCGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. A normalized version (NCI_CGAP_FT1) and a subtracted version (NCI_CGAP_FT2) of this library are also available. Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FT1

NAME: NCI_CGAP_FT1
LIB_ID: 2087
ORGANISM: human
ORGAN: macrophage
TISSUE: alveolar macrophage, pool of 81, challenged with different treatments
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGGCCATGCCGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized (a non-normalized version, NCI_CGAP_FT0, as well as a subtracted version, NCI_CGAP_FT2, of this library are also available). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

NCI_CGAP_FT2

NAME: NCI_CGAP_FT2
LIB_ID: 2168
ORGANISM: human
ORGAN: macrophage
TISSUE: alveolar macrophage, pool of 81, challenged with different treatments
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer (5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCGGCCATGCCGTTTTTTTTTTTTTTTTTTTT-3'); double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is subtracted (a non-normalized version, NCI_CGAP_FT0, and normalized version, NCI_CGAP_FT1, of this library are also available). Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP library.
||

DFCI-Vidal horfeome 1.1

NAME: DFCI-Vidal horfeome 1.1
LIB_ID: 2255
ORGANISM: human
ORGAN: mixed
HOST: DH10B TonA
VECTOR: pDONR223
V_TYPE: plasmid
RE_5': attB1.1
RE_3': attB2.1
DESCR:
Full open reading frame clones were created in the Gateway system by the M. Vidal laboratory (reference Genome Res. 2004 Oct;14(10B):2128-35) using MGC clones as templates. For PCR amplification, both forward and reverse ORF-specific primers for each MGC clone were designed automatically using the OSP program (Hillier and Green 1991). The forward primer starts from A of the ATG initiation codon, whereas the reverse primer starts from the second nucleotide of the termination codon. The reverse attB2.1 primers do not contain the last nucleotide of the termination codon, so as to allow generation of C-terminal fusion proteins. This library is specifically made of single colonies isolated at Lawrence Livermore National Laboratory from the transformed ORF pools. Clones were prepared by the M. Vidal laboratory (Dana Farber Cancer Institute) and generously donated for use by the NIH_MGC project.
||

MAPcL

NAME: MAPcL
LIB_ID: 1980
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: Pooled cell lines
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Directionally cloned into EcoRV (site destroyed upon cloning) and NotI. The library was size-selected and went through 26K fold of amplification. Average insert size is 1.8 kb. Library constructed from cell lines ZR-75-1, MCF7, SK-BR-3, MDA-MB-231, hTERT-HME1, LNCaP and subtracted against brain, liver, lung, kidney and muscle. cDNA Library constructed by Life Technologies (Invitrogen). The ZR-75-1, MCF7, SK-BR-3, MDA-MB-231, and LNCaP cell lines were acquired from ATCC. The hTERT-HME1 cell line was purchased from Clontech. REFERENCE: Kristi A. Egland, James J. Vincent, Robert Strausberg, Bungkook Lee, and Ira Pastan: Discovery of new breast cancer genes encoding membrane and secreted proteins. Manuscript submitted. (National Cancer Institute, National Institutes of Health).
||

NCI_CGAP_Sub1

NAME: NCI_CGAP_Sub1
LIB_ID: 1453
ORGANISM: human
ORGAN: mixed
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
||

NCI_CGAP_Sub2

NAME: NCI_CGAP_Sub2
LIB_ID: 1454
ORGANISM: human
ORGAN: mixed
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
||

NCI_CGAP_Sub3

NAME: NCI_CGAP_Sub3
LIB_ID: 1455
ORGANISM: human
ORGAN: mixed
TISSUE: This library is a mixture of the following 21 normalized or normalized/subtracted CGAP libraries: Co4, Pr22, Pr28, Co10, Co16, Kid5, Kid12, Kid3, Kid11, Lym2, Br2, Co8, CLL1, Lei2, Brn23, Lu5, Lu24, Lu19, GC4, GC6, and Brn25.
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
||

NCI_CGAP_Sub4

NAME: NCI_CGAP_Sub4
LIB_ID: 1456
ORGANISM: human
ORGAN: mixed
TISSUE: This library is derived from a mixture of 21 normalized or normalized/subtracted CGAP libraries: NCI_CGAP_Co4, Pr22, Pr28, Co10, Co16, Kid5, Kid12, Kid3, Kid11, Lym2, Br2, Co8, CLL1, Lei2, Brn23, Lu5, Lu24, Lu19, GC4, GC6, and Brn25. This is an additional subtraction from the NCI_CGAP_Sub2 library.
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
||

NCI_CGAP_Sub5

NAME: NCI_CGAP_Sub5
LIB_ID: 1499
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: pooled tissues: lung squamous cell carcinoma, poorly differentiated, 4 pooled tumors including primary and metastatic; B-cell; colon tumor; breast bulk tumor, pooled; colony tumor; bulk prostate; colon tumor RER+; 3 pooled germ cell tumors, including broad spectrum tumor types; 2 pooled kidneys; 2 pooled kidney tumors, clear cell type; glioblastoma, pooled; anaplastic oligodendroglioma; lymph node, follicular mixed small and large cell; neuroendocrine lunch carcinoid; colon adenocarcinoma; leiomyosarcoma, 2 pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
This subtracted library was derived from a pool of thefollowing 21 normalized and/or subtracted libraries: NCI_CGAP_Co4, Pr22, Pr28, Co10, Co16, Kid5, Kid12, Kid3, Kid11, Lym2, Br2, Co8, CLL1, Lei2, Brn23, Lu5, Lu24, Lu19, GC4, GC6, and Brn25. Library was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Sub6

NAME: NCI_CGAP_Sub6
LIB_ID: 1500
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: mixed
TISSUE: pooled tissues: ovary fibrotheoma; lung adenocarcinoma, poorly differentiated; medulloblastoma; flow-sorted tonsils
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
This subtracted library was derived from a pool of thefollowing four normalized libraries: NCI_CGAP_Ov18, Lu13, GCB1, and Brn50. Library was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Sub7

NAME: NCI_CGAP_Sub7
LIB_ID: 1501
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: mixed
TISSUE: pooled tissues: ovary fibrotheoma; lung adenocarcinoma, poorly differentiated; medulloblastoma; flow-sorted tonsils
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
This subtracted library was derived from NCI_CGAP_Sub6.Library was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Sub8

NAME: NCI_CGAP_Sub8
LIB_ID: 1599
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: pooled tissues: squamous cell carcinoma, poorly differentiated, 4 pooled tumors including primary and metastatic; 3 pooled germ cell tumors, including broad spectrum tumor types; glioblastoma, pooled; anaplastic oligodendroglioma; neuroendocrine lung carcinoid; leiomyosarcoma, 2 pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
This subtracted library was derived from a mixture of the followingnormalized and/or subtracted libraries: NCI_CGAP_Co4, Pr22, Pr28, Co10, Co16, Kid5, Kid12, Kid3, Kid11, Lym2, Br2, Co8, CLL1, Lei2, Brn23, Lu5, Lu24, Lu19, GC4, GC6, and Brn25. Library was constructed by Bento Soares and M.Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Sub9

NAME: NCI_CGAP_Sub9
LIB_ID: 1927
ORGANISM: human
STAGE: mixed
ORGAN: mixed
TISSUE: colonic mucosa (Crohn's disease), colonic mucosa (ulcerative colitis), fetal thymus, cervix, cervical adenosquamous carcinoma, ligament cells, prostate carcinoma, bladder carcinoma, brain oligodendroglioma
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
This subtracted library was derived from a pool of libraries and tissues: colonic mucosa from Crohn's disease (Soares/Dieckgraefe NHUC, normalized, tag TAGC), colonic mucosa from ulcerative colitis (Soares/Dieckgraefe NHCD, normalized, tag CGTC), fetal thymus (Soares NHFTh, normalized, tag AACG), cervix (Soares NHCe, normalized, tag GGGCC), cervical adenosquamous carcinoma (Soares NHCec, normalized, tag CGAAG), ligament cells, prostate carcinoma, bladder carcinoma, and brain oligodendroglioma (NCI_CGAP_Brn41, normalized, tag ATCAC). Library constructed by M. Bento Soares and Maria de Fatima Bonaldo (University of Iowa). Note: this is a NCI_CGAP Library.
||

NIH_MGC_115

NAME: NIH_MGC_115
LIB_ID: 1784
ORGANISM: human
STAGE: adult
ORGAN: mixed
TISSUE: pool of lung, 6 brains and testis
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source anonymous pool of 6 male brains, age range 23-27; 1 male lung, age 27; and 1 male testis, age 69. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.8 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 021. Note: this is a NIH_MGC Library. '
||

NIH_MGC_116

NAME: NIH_MGC_116
LIB_ID: 1785
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: mixed
TISSUE: pool of kidney (46 yo male), 2 stomachs (62 yo male, 70 yo female) and 3 colons (26 yo male, 49 yo female, 71 yo male)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source anonymous pool of 3 colons, age 26 yo male, 49 yo female, 71 yo male colon; 46 yo male kidney, and pool of 2 stomachs, 62 yo male and 70 yo female. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 023. Note: this is a NIH_MGC Library. '
||

NIH_MGC_117

NAME: NIH_MGC_117
LIB_ID: 1786
ORGANISM: human
SEX: female
STAGE: fetal
ORGAN: mixed
TISSUE: pool of liver (21 wk female), brain (24 wk male) and 6 hearts (20-24 wks male and female)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
RNA source anonymous pool of 24 week male brain, 21 week female liver, and six male and female hearts ranging from 20-24 weeks. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb, insert size range 1-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 024. Note: this is a NIH_MGC Library.
||

NIH_MGC_120

NAME: NIH_MGC_120
LIB_ID: 1789
ORGANISM: human
SEX: male
STAGE: adult
AGE: 28
ORGAN: mixed
TISSUE: liver and spleen
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source anonymous pool of spleen and pancreas from 28 yo male. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-2.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 025. Note: this is a NIH_MGC Library. '
||

NIH_MGC_122

NAME: NIH_MGC_122
LIB_ID: 1791
ORGANISM: human
SEX: female
STAGE: fetal
ORGAN: mixed
TISSUE: pool of lung (20 wk female) and spleen (pooled from 24 wk female and 20-22 wk males)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source anonymous pool of 24 week female lung, 16 week female spleen, and 20-22 week male spleens. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.4 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 026. Note: this is a NIH_MGC Library. '
||

NIH_MGC_145

NAME: NIH_MGC_145
LIB_ID: 2018
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: mixed tissues
HOST: DH10B
VECTOR: mixed
V_TYPE:
RE_5': multiple
RE_3': multiple
DESCR:
'ORFs were PCR-amplified and cloned into pcDNA3 and related vectors by the GPCR Consortium. For information about which gene each clone represents as well as the vectors and cloning sites (which vary by clone), please visit our anonymous ftp site at ftp://image.hudsonalpha.org/image/rearrayed_plates/IRBF-IRBI.preSV.data Note: this is a NIH_MGC Library. '
||

NIH_MGC_146

NAME: NIH_MGC_146
LIB_ID: 1960
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: mixed tissues
HOST: DH10B TonA
VECTOR: pcDNA3.1
V_TYPE: plasmid
RE_5': multiple
RE_3': multiple
DESCR:
ORFs were PCR-amplified (from IMAGE clones or from commercially available cDNA libraries) and cloned by the Guthrie cDNA Resource Center (www.guthrie.org/cDNA) into pcDNA3.1. For specific information on cloning sites (which vary by clone), please refer to the Guthrie website, using the Guthrie ID given in the file ftp://image.hudsonalpha.org/image/rearrayed_plates/IRBF.preSV.data
||

NIH_MGC_184

NAME: NIH_MGC_184
LIB_ID: 2029
ORGANISM: human
SEX: neither
ORGAN: mixed
TISSUE: glandular pool - thyroid, parathyroid, adrenal, cortex, pineal gland
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Library is oligo-dT primed and directionally cloned. cDNA was prepared from a glandular pool of tissues from thyoid, parathyroid, adrenal, cortex and pineal gland. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.38 kb (range 0.60-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_186

NAME: NIH_MGC_186
LIB_ID: 2030
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: Skin and meninges pool - skin, dura matter, pia matter, and choroid plexus
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Library is oligo-dT primed and directionally cloned. cDNA was prepared from a pooled samples of tissues from Skin, meninges, duramatter, pia matter and choroid plexus. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.47 kb (range 0.50-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_187

NAME: NIH_MGC_187
LIB_ID: 2031
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: Blood vessels, aorta, and basilar artery
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Library is oligo-dT primed and directionally cloned. cDNA was prepared from a pooled samples of tissues from Blood vessels, aorta, basilar and artery. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.10 kb (range 0.50-3.5 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_195

NAME: NIH_MGC_195
LIB_ID: 2042
ORGANISM: human
ORGAN: mixed
TISSUE: cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University).
HOST: DH10B TonA
VECTOR: pDNR-Dual
V_TYPE: plasmid
RE_5': loxP-SalI
RE_3': HindIII-loxP
DESCR:
Clones from this library have been PCR-amplified using gene-specific primers to contain the complete open reading frame (based on known gene sequences available from NCBI's RefSeq). Template for PCR is cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University). PCR products are directionally cloned into the loxP sites of the pDNR-Dual vector. Library constructed by Dr. Narayan Bhat, Earl Bere III and Hongling Liao (Gene Expression Laboratory, Research Technology Program, SAIC Frederick, NCI-Frederick, Frederick, MD 21702). For information on which gene each clone represents, please visit our anonymous ftp site at ftp://image.hudsonalpha.org/image/rearrayed_plates/IRBK-IRCC.preSV.data. Note: this is a NIH_MGC Library. '
||

NIH_MGC_217

NAME: NIH_MGC_217
LIB_ID: 2062
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: mixed
TISSUE: 3 cell lines - Myxoid Chondrosarcoma Grade II. Cell line CS-7 (passage 2), 105KD, JJ
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Average insert size 0.5-1kb. Library was constructed in the laboratory of M. Bento Soares. Note: this is a NIH_MGC Library.
||

NIH_MGC_218

NAME: NIH_MGC_218
LIB_ID: 2063
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: mixed
TISSUE: 3 pooled cell lines of Myxoid Chondrosarcoma Grade II: CS-7 (passage 2), 105KC, JJ
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of
||

NIH_MGC_219

NAME: NIH_MGC_219
LIB_ID: 2064
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: mixed
TISSUE: 3 pooled cell lines of Myxoid Chondrosarcoma Grade II. Cell lines CS-7 (passage 2), 105KC, JJ
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 2-3Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter priner 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Note: this is a NIH_MGC Library
||

NIH_MGC_220

NAME: NIH_MGC_220
LIB_ID: 2093
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: mixed
TISSUE: Pool of 3 cell lines of Myxoid Chondrosarcoma Grade II tissue. CS-7(passage 2), 105KC and JJ
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 0.5-1Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Average insert size 3-4kb Note: this is a NIH_MGC Library.||
||

NIH_MGC_221

NAME: NIH_MGC_221
LIB_ID: 2094
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: mixed
TISSUE: Pool of 3 cell lines from Chondrosarcoma Tumor. Cell lines CS-7 (passage 2), 105KC, JJ
HOST: DH10B TonA
VECTOR: pYX-Asc
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Library is oligo-dT primed and directionally cloned Denatured RNA was size fractionated on a 1% agarose gel. First strand cDNA synthesis was primed with oligo-dT primer containing a Not I site. Double strand cDNA was size selected according tomRNA size fraction, ligated with EcoR I adaptor, digested with Not I and then cloned directionally into pYX-Asc vector. Average insert size 4-5Kb. Adaptors 5'(AATTCGGCACGAGG)3' and 5'd (CCTCGTGCCG)3'. 3' Linker sequence - GCGGCCGCTGAGAGCC T18. Sequencing primers 3'end: T3 promoter primer 5'd (ATTAACCCTCACTAAAGGGA)3'. 5' End: T7 promoter primer 5'd (TAATACGACTCACTATAGGG)3'. Library was constructed in the laboratory of M. Bento Soares. Note: this is a NIH_MGC Library
||

NIH_MGC_241

NAME: NIH_MGC_241
LIB_ID: 2167
ORGANISM: human
ORGAN: mixed
TISSUE: mixed tissues from existing MGC libraries
HOST: XL-1 Blue
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'This library consists of a subset of clones from various MGC libraries that were found to have errors in their sequence, possibly the result of artifacts in cDNA library construction. These errors were repaired using site-directed mutagenesis by Modular Genetics Inc. (Woburn, MA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_244

NAME: NIH_MGC_244
LIB_ID: 2157
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: 20 tissues, mixed
HOST: XL10 Gold
VECTOR: pPCR-Script Amp SK(+)
V_TYPE: plasmid
RE_5': SrfI
RE_3': SrfI
DESCR:
Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Product from the RT-PCR was purified using QIAGEN PCR columns and ends were repaired for blunt-end ligation into the SrfI site of pPCR-Script Amp SK(+). Clones are oriented with 5' end nearest EcoRI site and 3' end nearest NotI site. Companion library NIH_MGC_277 has clones in the opposite orientation. Reference: Wu et al. (2004, Biotechniques in press). Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_245

NAME: NIH_MGC_245
LIB_ID: 2169
ORGANISM: human
ORGAN: mixed
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_271 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_271

NAME: NIH_MGC_271
LIB_ID: 2203
ORGANISM: human
ORGAN: mixed
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_245 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_277

NAME: NIH_MGC_277
LIB_ID: 2209
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: 20 tissues, mixed
HOST: DH10B TonA
VECTOR: pPCR-Script Amp SK(+)
V_TYPE: plasmid
RE_5': SrfI
RE_3': SrfI
DESCR:
Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Product from the RT-PCR was purified using QIAGEN PCR columns and ends were repaired for blunt-end ligation into the SrfI site of pPCR-Script Amp SK(+). Clones are oriented with 5' end nearest the NotI site and 3' end nearest the EcoRI site. Companion library NIH_MGC_244 has clones in the opposite orientation. Reference: Wu et al. (2004, Biotechniques in press). Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_282

NAME: NIH_MGC_282
LIB_ID: 2251
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: 20 tissues, mixed
HOST: DH10B TonA
VECTOR: pCR-BluntII-TOPO
V_TYPE: plasmid
RE_5': TOPO sites
RE_3': TOPO sites
DESCR:
Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Inserts are cloned into the TOPO site and oriented with 5' end nearest the SpeI site and 3' end nearest the PstI site. Inserts can be excised using EcoRI. Companion library NIH_MGC_283 has clones in the opposite orientation. Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a NIH_MGC Library.
||

NIH_MGC_283

NAME: NIH_MGC_283
LIB_ID: 2252
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: 20 tissues, mixed
HOST: DH10B TonA
VECTOR: pCR-BluntII-TOPO
V_TYPE: plasmid
RE_5': TOPO sites
RE_3': TOPO sites
DESCR:
Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Inserts are cloned into the TOPO site and oriented with 5' end nearest the PstI site and 3' end nearest the SpeI site. Inserts can be excised using EcoRI. Companion library NIH_MGC_282 has clones in the opposite orientation. Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a NIH_MGC Library.
||

NIH_MGC_314

NAME: NIH_MGC_314
LIB_ID: 2274
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and heart
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_315 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_315

NAME: NIH_MGC_315
LIB_ID: 2275
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and heart
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_314 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_316

NAME: NIH_MGC_316
LIB_ID: 2296
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and heart
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_317 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_317

NAME: NIH_MGC_317
LIB_ID: 2297
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and heart
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_316 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_318

NAME: NIH_MGC_318
LIB_ID: 2276
ORGANISM: human
ORGAN: mixed
TISSUE: pool of brain, heart and lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_319 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_319

NAME: NIH_MGC_319
LIB_ID: 2277
ORGANISM: human
ORGAN: mixed
TISSUE: pool of brain, heart and lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_318 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_320

NAME: NIH_MGC_320
LIB_ID: 2298
ORGANISM: human
ORGAN: mixed
TISSUE: pool of brain, heart and lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_321 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_321

NAME: NIH_MGC_321
LIB_ID: 2299
ORGANISM: human
ORGAN: mixed
TISSUE: pool of brain, heart and lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_320 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_322

NAME: NIH_MGC_322
LIB_ID: 2278
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex and lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_323 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_323

NAME: NIH_MGC_323
LIB_ID: 2279
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex and lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_322 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_324

NAME: NIH_MGC_324
LIB_ID: 2300
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex and lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_325 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_325

NAME: NIH_MGC_325
LIB_ID: 2301
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex and lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_324 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_326

NAME: NIH_MGC_326
LIB_ID: 2280
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and placenta
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_327 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_327

NAME: NIH_MGC_327
LIB_ID: 2281
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and placenta
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_326 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_328

NAME: NIH_MGC_328
LIB_ID: 2302
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and placenta
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_329 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_329

NAME: NIH_MGC_329
LIB_ID: 2303
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung and placenta
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_328 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_330

NAME: NIH_MGC_330
LIB_ID: 2306
ORGANISM: human
ORGAN: mixed
TISSUE: cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University).
HOST: DH10B TonA
VECTOR: pCR-BluntII-TOPO
V_TYPE: plasmid
RE_5': TOPO sites
RE_3': TOPO sites
DESCR:
Clones from this library have been PCR-amplified using gene-specific primers to contain the complete open reading frame (based on known gene sequences available from NCBI's RefSeq). Template for PCR is cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University). PCR products are non-directionally cloned into the TOPO sites of the pCR-BluntII-TOPO vector. Library constructed by Dr. Narayan Bhat, Earl Bere III and Hongling Liao (Gene Expression Laboratory, Research Technology Program, SAIC Frederick, NCI-Frederick, Frederick, MD 21702). For information on which gene each clone represents, and the orientation in which it's cloned (determined by sequence analysis; this library represents the forward direction and NIH_MGC_332 represents the reverse direction) please visit our anonymous ftp site at ftp://image.hudsonalpha.org/image/rearrayed_plates/IRBK-IRCC.preSV.data Note: this is a NIH_MGC Library.
||

NIH_MGC_332

NAME: NIH_MGC_332
LIB_ID: 2317
ORGANISM: human
ORGAN: mixed
TISSUE: cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University).
HOST: DH10B TonA
VECTOR: pCR-BluntII-TOPO
V_TYPE: plasmid
RE_5': TOPO sites
RE_3': TOPO sites
DESCR:
Clones from this library have been PCR-amplified using gene-specific primers to contain the complete open reading frame (based on known gene sequences available from NCBI's RefSeq). Template for PCR is cDNA derived from either pooled cytoplasmic polyA RNA from 30 cells lines or pooled total RNA from 10 different tissues (from BD Biosciences/Clontech and Washington University). PCR products are non-directionally cloned into the TOPO sites of the pCR-BluntII-TOPO vector. Library constructed by Dr. Narayan Bhat, Earl Bere III and Hongling Liao (Gene Expression Laboratory, Research Technology Program, SAIC Frederick, NCI-Frederick, Frederick, MD 21702). For information on which gene each clone represents, and the orientation in which it's cloned (determined by sequence analysis; this library represents the reverse direction and NIH_MGC_330 represents the forward direction) please visit our anonymous ftp site at ftp://image.hudsonalpha.org/image/rearrayed_plates/IRBK-IRCC.preSV.data Note: this is a NIH_MGC Library.
||

NIH_MGC_333

NAME: NIH_MGC_333
LIB_ID: 2322
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex, heart, lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_334 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_334

NAME: NIH_MGC_334
LIB_ID: 2323
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex, heart, lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_333 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_335

NAME: NIH_MGC_335
LIB_ID: 2324
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex, heart, lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_336 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_336

NAME: NIH_MGC_336
LIB_ID: 2325
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebral cortex, heart, lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_335 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_337

NAME: NIH_MGC_337
LIB_ID: 2326
ORGANISM: human
SEX: both
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, testis
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_338 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_338

NAME: NIH_MGC_338
LIB_ID: 2327
ORGANISM: human
SEX: both
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, testis
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_337 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_339

NAME: NIH_MGC_339
LIB_ID: 2328
ORGANISM: human
SEX: both
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, testis
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_340 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_340

NAME: NIH_MGC_340
LIB_ID: 2329
ORGANISM: human
SEX: both
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, testis
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_339 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_341

NAME: NIH_MGC_341
LIB_ID: 2330
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, ovary
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_342 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_342

NAME: NIH_MGC_342
LIB_ID: 2331
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, ovary
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_341 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_343

NAME: NIH_MGC_343
LIB_ID: 2332
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, ovary
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_344 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_344

NAME: NIH_MGC_344
LIB_ID: 2333
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, uterus, ovary
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_343 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_345

NAME: NIH_MGC_345
LIB_ID: 2334
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum and lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_346 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_346

NAME: NIH_MGC_346
LIB_ID: 2335
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum and lung
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_345 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_347

NAME: NIH_MGC_347
LIB_ID: 2336
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum and lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_348 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_348

NAME: NIH_MGC_348
LIB_ID: 2337
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum and lung
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_347has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_349

NAME: NIH_MGC_349
LIB_ID: 2344
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus, placenta
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_350 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_350

NAME: NIH_MGC_350
LIB_ID: 2345
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus, placenta
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_349 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_351

NAME: NIH_MGC_351
LIB_ID: 2346
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus, placenta
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_352 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_352

NAME: NIH_MGC_352
LIB_ID: 2347
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus, placenta
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_351 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_353

NAME: NIH_MGC_353
LIB_ID: 2348
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, bladder, kidney
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_354 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_354

NAME: NIH_MGC_354
LIB_ID: 2349
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, bladder, kidney
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_353 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_355

NAME: NIH_MGC_355
LIB_ID: 2350
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, bladder, kidney
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_356 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_356

NAME: NIH_MGC_356
LIB_ID: 2351
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, bladder, kidney
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_355 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_357

NAME: NIH_MGC_357
LIB_ID: 2352
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_358 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_358

NAME: NIH_MGC_358
LIB_ID: 2353
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_357 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_359

NAME: NIH_MGC_359
LIB_ID: 2354
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_360 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_360

NAME: NIH_MGC_360
LIB_ID: 2355
ORGANISM: human
ORGAN: mixed
TISSUE: pool of bladder, uterus
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_359 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_361

NAME: NIH_MGC_361
LIB_ID: 2356
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_362 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_362

NAME: NIH_MGC_362
LIB_ID: 2357
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_361 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_363

NAME: NIH_MGC_363
LIB_ID: 2358
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_364 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_364

NAME: NIH_MGC_364
LIB_ID: 2359
ORGANISM: human
ORGAN: mixed
TISSUE: pool of cerebellum, kidney, placenta, testis, lung, colon, liver, heart, thyroid, bladder, uterus
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_363 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_397

NAME: NIH_MGC_397
LIB_ID: 2434
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: 20 tissues, mixed
HOST: DH10B TonA
VECTOR: pCR-BluntII-TOPO
V_TYPE: plasmid
RE_5': TOPO sites
RE_3': TOPO sites
DESCR:
Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Inserts are cloned into the TOPO site and oriented with 5' end nearest the SpeI site and 3' end nearest the PstI site. Inserts can be excised using EcoRI. Companion library NIH_MGC_398 has clones in the opposite orientation. Clones from this library represent predicted genes. Library constructed at Baylor College of Medicine, human Genome Sequencing Center. Note: this is a NIH_MGC Library.
||

NIH_MGC_398

NAME: NIH_MGC_398
LIB_ID: 2435
ORGANISM: human
SEX: unknown
ORGAN: mixed
TISSUE: 20 tissues, mixed
HOST: DH10B TonA
VECTOR: pCR-BluntII-TOPO
V_TYPE: plasmid
RE_5': TOPO sites
RE_3': TOPO sites
DESCR:
Library was constructed from RT-PCR products generated from the BD Biosciences Clontech Human Total RNA Master Panel II (consisting of RNAs from 20 different tissues). Inserts are cloned into the TOPO site and oriented with 5' end nearest the PstI site and 3' end nearest the SpeI site. Inserts can be excised using EcoRI. Companion library NIH_MGC_397 has clones in the opposite orientation. Clones from this library represent predicted genes. Library constructed at Baylor College of Medicine, Human Genome Sequencing Center. Note: this is a NIH_MGC Lib rary.
||

Soares 1NFLS

NAME: Soares 1NFLS
LIB_ID: 59
ORGANISM: human
SEX: male
STAGE: fetal
ORGAN: mixed
TISSUE: spleen and liver
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Pac I - oligo(dT) primer[5' AACTGGAAGAATTAATTAAAGATCTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares 1NFLS-S1

NAME: Soares 1NFLS-S1
LIB_ID: 251
ORGANISM: human
SEX: male
STAGE: fetal
ORGAN: mixed
TISSUE: spleen and liver
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
DNAs from I.M.A.G.E. clones 66696-67079 and 108168-112775(which are from the Soares 1NFLS library) were used as a driver in a subtraction of the 1NFLS library to make the 1NFLS-S1 library.
||

Soares NFL_T_GBC_S1

NAME: Soares NFL_T_GBC_S1
LIB_ID: 689
ORGANISM: human
ORGAN: mixed
TISSUE: pool of lung (19 wks post conception), testis (adult) and flow-sorted tonsils (adult)
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Equal amounts of plasmid DNA from three normalized libraries(fetal lung NbHL19W, testis NHT, and B-cell NCI_CGAP_GCB1) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 297480-302087, 682632-687239, 726408-728711, and 729096-731399. Subtraction by Bento Soares and M. Fatima Bonaldo.
||

Soares NSF-F8-9W-OT-PA-P-S1

NAME: Soares NSF-F8-9W-OT-PA-P-S1
LIB_ID: 788
ORGANISM: human
ORGAN: mixed
TISSUE: pool of fetus (8-9 wks postconception), full-term placenta obtained at birth, ovary papillary serous cystadenocarcinoma grade III tumor with surface extensions and metastases (36 yo female), 3 pooled parathyroid glands with primary hyperparathyroidism, fibroblast senescent cells
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Equal amounts of plasmid DNA from five normalized librarieswere mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 5 libraries. The pools consisted of the following libraries and cloneIDs: Soares NbHSF pool 1: 309384-310919, 323208-325895 Soares Nb2HP pool 1: 145032-147335, 147720-148103, 148872-149255, 150024- 150407, 151176-152327 Soares Nb2HF8-9W pool 1: 758280-760583, 772104-774407 Soares NbHPA pool 1: 304776-306311, 320136-322823, 326280-326663 Soares NbHOT pool 1: 723720-726407, 739080-740999 Subtraction by Bento Soares and M. Fatima Bonaldo.
||

Soares NbHPU

NAME: Soares NbHPU
LIB_ID: 253
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: mixed
TISSUE: colon tumor, uterus
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCTTTTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares NhHMPu-S1

NAME: Soares NhHMPu-S1
LIB_ID: 363
ORGANISM: human
ORGAN: mixed
TISSUE: pool of heart (19 wks postconception), melanocyte (adult) and uterus (adult)
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Equal amounts of plasmid DNA from three normalized libraries(melanocyte 2NbHM, pregnant uterus NbHPU, and fetal heart NbHH19W) were mixed, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from pools of 5,000 clones made from the same 3 libraries. The pools consisted of I.M.A.G.E. clones 260232-265223, 340488-345479, and 484488-489479. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

NCI_CGAP_HN10

NAME: NCI_CGAP_HN10
LIB_ID: 1387
ORGANISM: human
ORGAN: mouth
TISSUE: carcinoma in situ from retromolar trigone
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN15

NAME: NCI_CGAP_HN15
LIB_ID: 1460
ORGANISM: human
ORGAN: mouth
TISSUE: leukoplakia of the buccal mucosa
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from leukoplakia, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN16

NAME: NCI_CGAP_HN16
LIB_ID: 1461
ORGANISM: human
ORGAN: mouth
TISSUE: moderate to poorly differentiated invasive carcinoma of the retromolar trigone
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN7

NAME: NCI_CGAP_HN7
LIB_ID: 1395
ORGANISM: human
ORGAN: mouth
TISSUE: normal squamous epithelium from floor of mouth
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN8

NAME: NCI_CGAP_HN8
LIB_ID: 1390
ORGANISM: human
ORGAN: mouth
TISSUE: well-differentiated invasive carcinoma, floor of mouth
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN9

NAME: NCI_CGAP_HN9
LIB_ID: 1391
ORGANISM: human
ORGAN: mouth
TISSUE: normal squamous epithelium from retromolar trigone
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

Barstead HPL-RB8

NAME: Barstead HPL-RB8
LIB_ID: 1323
ORGANISM: human
SEX: female
STAGE: adult
AGE: 27
ORGAN: muscle
TISSUE: Psoas muscle
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to Eco RI adaptors [5' AATTCACTAGTAAC 3' and 5' GTTACTAGTG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

Clontech muscle (HL3000s)

NAME: Clontech muscle (HL3000s)
LIB_ID: 74
ORGANISM: human
STAGE: adult
ORGAN: muscle
TISSUE: skeletal muscle
HOST: MC1061/P3
VECTOR: pcDNAI
V_TYPE: plasmid
RE_5': BstXI
RE_3': NotI
DESCR:
||

NCI_CGAP_AR1

NAME: NCI_CGAP_AR1
LIB_ID: 407
ORGANISM: human
STAGE: adult
ORGAN: muscle
TISSUE: bulk alveolar rhabdomyosarcoma
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Alv1

NAME: NCI_CGAP_Alv1
LIB_ID: 474
ORGANISM: human
STAGE: adult
ORGAN: muscle
TISSUE: alveolar rhabdomyosarcoma, striated muscle
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from alveolar rhabdomyosarcoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIH_MGC_123

NAME: NIH_MGC_123
LIB_ID: 1792
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: muscle
TISSUE: pool of skeletal muscle (57 yo female), breast (58 yo female) and heart (24 yo male)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source anonymous pool of male heart, age 24; female skeletal muscle, age 57, and female breast, age 58. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.5 kb, insert size range 1-3 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 022. Note: this is a NIH_MGC Library. '
||

NIH_MGC_17

NAME: NIH_MGC_17
LIB_ID: 1429
ORGANISM: human
ORGAN: muscle
TISSUE: rhabdomyosarcoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_183

NAME: NIH_MGC_183
LIB_ID: 2028
ORGANISM: human
SEX: unknown
ORGAN: muscle
TISSUE: Muscle pool (cardiac and skeletal muscle)
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.7. Library was constructed by Invitrogen. Note: this is a NIH_MGC Library. '
||

NIH_MGC_81

NAME: NIH_MGC_81
LIB_ID: 1644
ORGANISM: human
ORGAN: muscle
TISSUE: skeletal muscle
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.55 kb (range 1.0-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

Stratagene skeletal muscle (937209)

NAME: Stratagene skeletal muscle (937209)
LIB_ID: 270
ORGANISM: human
STAGE: adult
ORGAN: muscle
TISSUE: skeletal muscle from patient with malignant hyperthermia
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Skeletal muscle from patient with malignant hyperthermia. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Weizmann olfactory epithelium

NAME: Weizmann olfactory epithelium
LIB_ID: 121
ORGANISM: human
SEX: female
STAGE: adult
AGE: 35
ORGAN: olfactory epithelium
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Olfactory epithelium, normal. Average insert size: 0.8 kb; Uni-ZAP XR Vector. Library constructed by N. Walker, D. Lancet, Weizmann Institute of Science. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

NCI_CGAP_Ov1

NAME: NCI_CGAP_Ov1
LIB_ID: 405
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: bulk tumor
HOST: DH10B
VECTOR: pCMV-SPORT2
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov10

NAME: NCI_CGAP_Ov10
LIB_ID: 786
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: poorly differentiated adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.3 kb. Life Technologies catalog #: 10983-013 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov11

NAME: NCI_CGAP_Ov11
LIB_ID: 787
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: well differentiated papillary adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.6 kb. Life Technologies catalog #: 10983-013 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov18

NAME: NCI_CGAP_Ov18
LIB_ID: 1345
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: ovary, fibrotheoma
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGCGACATTTTTTTTTTTTTTTTTT 3']; double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov2

NAME: NCI_CGAP_Ov2
LIB_ID: 473
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: invasive tumor
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive ovarian tumor, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov23

NAME: NCI_CGAP_Ov23
LIB_ID: 1239
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: primary tumors, metastasis positive, 5 pooled including mixed Mullerian tumor, papillary serous, clear cell, spindle cell
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.35 kb. Tumor types include: mixed Mullerian tumor, papillary serous, clear cell, spindle cell. All are primary tumors, metastasis positive. Life Technologies catalog #: 11534-013 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov26

NAME: NCI_CGAP_Ov26
LIB_ID: 1206
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: papillary serous ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from papillary serous ovarian carcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov31

NAME: NCI_CGAP_Ov31
LIB_ID: 1236
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: papillary serous ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from ovarian carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Non-amplified library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov32

NAME: NCI_CGAP_Ov32
LIB_ID: 1237
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: papillary serous ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from ovarian carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Non-amplified library. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov33

NAME: NCI_CGAP_Ov33
LIB_ID: 1287
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: borderline ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from borderline ovarian carcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. CITATION: National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index CONT_NAME: Robert Strausberg, Ph.D. COMMENT: cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: I.M.A.G.E. Consortium, LLNL DNA Sequencing by: Washington University Genome Sequencing Center Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov34

NAME: NCI_CGAP_Ov34
LIB_ID: 1286
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: borderline ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from borderline ovarian carcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. CITATION: National Cancer Institute, Cancer Genome Anatomy Project (CGAP), Tumor Gene Index CONT_NAME: Robert Strausberg, Ph.D. COMMENT: cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. cDNA Library Arrayed by: I.M.A.G.E. Consortium, LLNL DNA Sequencing by: Washington University Genome Sequencing Center Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov35

NAME: NCI_CGAP_Ov35
LIB_ID: 1330
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: primary tumors, metastasis positive, 5 pooled including mixed Mullerian tumor, papillary serous, clear cell, spindle cell
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
This library represents the normalized version of NCI_CGAP_Ov23. Cloned unidirectionally. Primer: Oligo dT. Average insert size 0.86 kb. Tumor types include: mixed Mullerian tumor, papillary serous, clear cell, spindle cell. All are primary tumors, metastasis positive. Constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov36

NAME: NCI_CGAP_Ov36
LIB_ID: 1285
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: borderline ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from borderline ovarian carcinoma, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov37

NAME: NCI_CGAP_Ov37
LIB_ID: 1301
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: early stage papillary serous ovarian carcinoma
HOST: DH10B
VECTOR: pAMP1
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from ovary carcinoma tissue, cDNA made by oligo-dT priming. Directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 400 bp. Primary library, non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov38

NAME: NCI_CGAP_Ov38
LIB_ID: 1349
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: pool of 3 cell lines: transfected epithelium, untransfected epithelium, epithelium with BRCA1 mutation (5256delG)
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov39

NAME: NCI_CGAP_Ov39
LIB_ID: 1393
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: papillary serous ovarian carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov40

NAME: NCI_CGAP_Ov40
LIB_ID: 1394
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: endometrioid ovarian metastasis
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
cDNA made by oligo-dT priming. Non-directionally cloned into the UDG sites of pAMP10. Size-selected on agarose gel, average insert size 500 bp. Primary library; non-amplified. cDNA Library Preparation: David B. Krizman, Ph.D (NCI). Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov41

NAME: NCI_CGAP_Ov41
LIB_ID: 1466
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: serous papillary tumor
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov5

NAME: NCI_CGAP_Ov5
LIB_ID: 592
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: surface epithelium
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal ovarian epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov6

NAME: NCI_CGAP_Ov6
LIB_ID: 593
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: cortical stroma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal ovarian cortical stroma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Ov8

NAME: NCI_CGAP_Ov8
LIB_ID: 731
ORGANISM: human
ORGAN: ovary
TISSUE: serous adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Life Technologies catalog #: 10982-015 Note: this is a NCI_CGAP Library.
||

NICHD_Hs_Ov1

NAME: NICHD_Hs_Ov1
LIB_ID: 2057
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: Granulosa lutein cells aspirated from preovulator follicles of normal cycling women undergoing ovulation induction of infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. Polled tissures from Percoll purified granulosa lutein cells.
HOST: DH10B TonA
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
Library is oligo-dT primed and directionally cloned. Granulosa lutein cells aspirated from preovulatory folicles of normal cycling women undergoing ovulation induction for infertility due to male factor and normal doners. The cells were from follicles stimulated with Lupron, FSH and hCG. 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 2.23 kb (range 1.0-4.5 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
||

NIH_MGC_109

NAME: NIH_MGC_109
LIB_ID: 1860
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: teratocarcinoma, cell line
HOST: DH10B TonA
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_125

NAME: NIH_MGC_125
LIB_ID: 1862
ORGANISM: human
SEX: female
STAGE: adult
ORGAN: ovary
TISSUE: pool of 3 ovaries
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' RNA source pool of three ovaries, from females ranging in age from 38 to 49 yo. Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb, insert size range 1-3.5 kb. Library is normalized and enriched for full-length clones and was constructed by C. Gruber (Invitrogen). Research Genetics tracking code 036. Note: this is a NIH_MGC Library. '
||

NIH_MGC_66

NAME: NIH_MGC_66
LIB_ID: 1615
ORGANISM: human
SEX: female
ORGAN: ovary
TISSUE: adenocarcinoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.8 kb. Library constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_9

NAME: NIH_MGC_9
LIB_ID: 1426
ORGANISM: human
ORGAN: ovary
TISSUE: ovarian adenocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

Soares NbHOT

NAME: Soares NbHOT
LIB_ID: 116
ORGANISM: human
SEX: female
STAGE: adult
AGE: 36
ORGAN: ovary
TISSUE: papillary serous cystadenocarcinoma grade III tumor with surface extensions and metastases
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA (prepared from a papillary serous cystadenocarcinomagrade III tumor with surface extensions and metastases) was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGGTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot53. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Stratagene ovary (937217)

NAME: Stratagene ovary (937217)
LIB_ID: 65
ORGANISM: human
SEX: female
STAGE: adult
AGE: 49
ORGAN: ovary
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Total ovary tissue, normal, caucasian. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Stratagene ovary tumor (937219)

NAME: Stratagene ovary tumor (937219)
LIB_ID: 274
ORGANISM: human
SEX: female
STAGE: adult
AGE: 64
ORGAN: ovary
TISSUE: papillary serous carcinoma, isolated from ascites
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Papillary serous carcinoma, isolated from ascites, 64 year old caucasian. Average insert size: 0.8 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Barstead HPL-RB1

NAME: Barstead HPL-RB1
LIB_ID: 719
ORGANISM: human
SEX: female
STAGE: adult
AGE: 34
ORGAN: pancreas
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [AATTCGGATCCATG and CATGGATCCG], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

HR85 islet

NAME: HR85 islet
LIB_ID: 1775
ORGANISM: human
ORGAN: pancreas
TISSUE: pancreatic islet
HOST: DH10B TonA
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': XhoI
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Size-selected on agarose gel for averageinsert size 1.5 kb. 5' XhoI site was destroyed after directional cloning. Library was amplified once. Please contact Hiroshi Inoue, MD/PhD for further information on this library (Metabolism Division, Permutt Laboratory, Washington University School of Medicine, Box 8127, 660 S Euclid Ave, St. Louis, MO 63110). Note: this is a Washington University Pancreas EST project library.
||

Human insulinoma

NAME: Human insulinoma
LIB_ID: 1749
ORGANISM: human
ORGAN: pancreas
TISSUE: insulinoma
HOST: GeneHogs DH10B
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Constructed with lambda ZAPII system (Stratagene) by Dr. J.Ferrer, in vivo mass-excised to pBluescript SK- by Dr. H. Inoue following the Washington University protocol (http://genome.wustl.edu/est/lambda_protocol.shtml). Please contact Hiroshi Inoue, MD/PhD for further information on this library (Metabolism Division, Permutt Laboratory, Washington University School of Medicine, Box 8127, 660 S Euclid Ave, St. Louis, MO 63110). Note: this is a Washington University Pancreas EST project library.
||

Melton human islets HIZ1

NAME: Melton human islets HIZ1
LIB_ID: 1909
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: pancreas
TISSUE: Islets of Langerhans, pooled
HOST: TOP10
VECTOR: pZERO-2
V_TYPE: plasmid
RE_5': XhoI
RE_3': NotI
DESCR:
Library constructed using SuperScript Plasmid Library kit (Life Technologies) and 5' linker sequences as follows. 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. cDNA made by oligo-dT priming using 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. XhoI site destroyed during cloning (excise insert using XbaI/NotI). Size-selected by column fractionation; average insert size 1.59kb. Primary library, unamplified. Library constructed and arrayed in the laboratory of D. Melton, Ph.D. (Harvard University).
||

Melton human pancreatic islets

NAME: Melton human pancreatic islets
LIB_ID: 1908
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: pancreas
TISSUE: Islets of Langerhans, pooled
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Library constructed using SuperScript Plasmid Library kit (Life Technologies) and 5' linker sequences as follows. 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. cDNA made by oligo-dT priming using 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size-selected by column fractionation; average insert size 1.08kb. Primary library, unamplified. Library constructed and arrayed in the laboratory of D. Melton, Ph.D. (Harvard University).
||

Melton normalized human islet 4 N4-HIS1

NAME: Melton normalized human islet 4 N4-HIS1
LIB_ID: 1898
ORGANISM: human
SEX: both
STAGE: adult
ORGAN: pancreas
TISSUE: Islets of Langerhans, pooled
HOST: DH10B
VECTOR: pSPORT1
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Starting library constructed using SuperScript Plasmid Library kit (Life Technologies) and 5' linker sequences as follows. 5'-TCGACCCACGCGTCCG-3' and 3'-GGGTGCGCAGGC-5'. cDNA made by oligo-dT priming using 5'-PGACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size-selected by column fractionation; average insert size 1.08 kb. Library was amplified once on solid support and plasmid DNA from library was prepared. The library DNA was normalized by method #4 from Bonaldo, Lennon, and Soares (1996 Genome Research 6:791-806); 0.5 microgram single-stranded library plasmid DNA was mixed with 5 micrograms PCR product representing library inserts and hybridized to an Ecot of 20. Single-stranded (unhybridized) plasmids were isolated by hydroxyapatite chromatography and used to make this library. Library arrayed and constructed in the laboratory of D. Melton, Ph.D. (Harvard University).
||

NCI_CGAP_PI1

NAME: NCI_CGAP_PI1
LIB_ID: 531
ORGANISM: human
STAGE: adult
ORGAN: pancreas
TISSUE: pooled pancreatic islet tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pooled pancreatic islet tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pan1

NAME: NCI_CGAP_Pan1
LIB_ID: 1275
ORGANISM: human
STAGE: adult
ORGAN: pancreas
TISSUE: adenocarcinoma
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.72 kb. Life Technologies catalog #: 11548-013 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pan3

NAME: NCI_CGAP_Pan3
LIB_ID: 1467
ORGANISM: human
ORGAN: pancreas
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NIH_MGC_110

NAME: NIH_MGC_110
LIB_ID: 1814
ORGANISM: human
ORGAN: pancreas
TISSUE: ductal carcinoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_39

NAME: NIH_MGC_39
LIB_ID: 1571
ORGANISM: human
ORGAN: pancreas
TISSUE: adenocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_42

NAME: NIH_MGC_42
LIB_ID: 1706
ORGANISM: human
ORGAN: pancreas
TISSUE: epithelioid carcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_70

NAME: NIH_MGC_70
LIB_ID: 1619
ORGANISM: human
ORGAN: pancreas
TISSUE: epithelioid carcinoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Library constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_78

NAME: NIH_MGC_78
LIB_ID: 1652
ORGANISM: human
ORGAN: pancreas
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.2 kb (range 0.5-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

Stratagene pancreas tumor (937208)

NAME: Stratagene pancreas tumor (937208)
LIB_ID: 269
ORGANISM: human
STAGE: adult
ORGAN: pancreas
TISSUE: adenocarcinoma cell line CF Pac-1
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pancreatic adenocarcinoma cell line CF Pac-1. Average insert size: 1.0 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Takeda/Bell pancreatic islet

NAME: Takeda/Bell pancreatic islet
LIB_ID: 228
ORGANISM: human
STAGE: adult
ORGAN: pancreas
TISSUE: pooled pancreatic islets (90% pure)
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Normal adult pancreatic islets, estimated to be 90% pure, were source of mRNA. Uni-ZAP XR Vector. Reference: Human Molecular Genetics 2, 1793-1798 (1993) Library constructed by J. Takeda, G. Bell. ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Soares NbHPA

NAME: Soares NbHPA
LIB_ID: 218
ORGANISM: human
STAGE: adult
ORGAN: parathyroid
TISSUE: three pooled with primary hyperparathyroidism
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA (prepared from RNA of 3 parathryoid adenomasfrom adults with primary hyperparathyroidism, provided by Stephen Marx and Allen Spiegel, NIH) was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCACCAATTTTTTTTTTTTTTTTTTTT 3'], double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed by Bento Soares and M. Fatima Bonaldo.
||

Human Anterior Horn

NAME: Human Anterior Horn
LIB_ID: 2048
ORGANISM: human
SEX: unknown
ORGAN: peripheral nervous system
TISSUE: anterior horn
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 2.1 kb. Library was constructed by Invitrogen and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine).
||

Lupski dorsal root ganglion

NAME: Lupski dorsal root ganglion
LIB_ID: 1514
ORGANISM: human
SEX: male
STAGE: adult
AGE: 36
ORGAN: peripheral nervous system
TISSUE: dorsal root ganglia
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.7 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine) and is available through Life Technologies.
||

Lupski sciatic nerve

NAME: Lupski sciatic nerve
LIB_ID: 1513
ORGANISM: human
SEX: male
STAGE: adult
AGE: 70
ORGAN: peripheral nervous system
TISSUE: sciatic nerve
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.87 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine) and is available through Life Technologies.
||

Lupski sympathetic trunk

NAME: Lupski sympathetic trunk
LIB_ID: 1660
ORGANISM: human
SEX: male
STAGE: adult
AGE: 16
ORGAN: peripheral nervous system
TISSUE: sympathetic trunk
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
cDNA made by oligo-dT priming. Directionally cloned using the following adaptors: 5'-TCGACCCACGCGTCCG-3' and 5'-GACTAGTTCTAGATCGCGAGCGGCCGCCCT(15)-3'. Size selected > 1 kb for average insert length 1.9 kb. This is a primary library, non-amplified. Library constructed by Life Technologies and donated by J. Lupski, M.D./Ph.D. (Baylor College of Medicine); available through Life Technologies.
||

NCI_CGAP_PNS1

NAME: NCI_CGAP_PNS1
LIB_ID: 734
ORGANISM: human
ORGAN: peripheral nervous system
TISSUE: dorsal root gangion
HOST: DH10B
VECTOR: pCMV-SPORT4
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.1 kb. Life Technologies catalog #: 10988-012 Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN17

NAME: NCI_CGAP_HN17
LIB_ID: 1517
ORGANISM: human
ORGAN: pharynx
TISSUE: nasopharyngeal epithelium
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal nasopharyngeal epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN19

NAME: NCI_CGAP_HN19
LIB_ID: 1519
ORGANISM: human
ORGAN: pharynx
TISSUE: nasopharyngeal epithelium
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from nasopharyngeal epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_HN4

NAME: NCI_CGAP_HN4
LIB_ID: 765
ORGANISM: human
ORGAN: pharynx
TISSUE: squamous cell carcinoma
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5 kb. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' (GA)10ACTAGTCTCGAGTTTTTTTTTTTTTTTTTT 3' Library constructed by Stratagene. Note: this is a NCI_CGAP Library.
||

Soares 1NbHPG

NAME: Soares 1NbHPG
LIB_ID: 114
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: pineal gland
TISSUE: pool of 3
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA (prepared from post mortem tissue) was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTTTTTTTTTTTTTTTTTT 3'], double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot40. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares 3NbHPG

NAME: Soares 3NbHPG
LIB_ID: 115
ORGANISM: human
SEX: unknown
STAGE: adult
ORGAN: pineal gland
TISSUE: 3 pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA (prepared from post mortem tissue) was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCGTTTTTTTTTTTTTTTTTT 3'], double- stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, to Cot38. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

NIH_MGC_179

NAME: NIH_MGC_179
LIB_ID: 2024
ORGANISM: human
SEX: unknown
ORGAN: pituitary gland
TISSUE: pituitary
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.1
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
' Library is oligo-dT primed and directionally cloned (EcoRV site is destroyed upon cloning). Average insert size 1.1 kb. Library was constructed by Invitrogen. Note: this is a NIH_MGC Library. '
||

Gatewood hydatidiform mole

NAME: Gatewood hydatidiform mole
LIB_ID: 745
ORGANISM: human
STAGE: placental
ORGAN: placenta
TISSUE: hydatidiform mole
HOST: TOP10
VECTOR: pCDNAII
V_TYPE: phagemid
RE_5': NotI
RE_3': NotI
DESCR:
Hydatidiform mole is an abnormal human pregnancy which is encoded exclusively by the paternal genome. Complete hydatidiform mole consists of placental tissue only. First stand synthesis was initiated using a polyT-NotI primer. Following addition of BstX1 linkers, the completed cDNA was restricted with NotI. The restricted cDNA was size fractionated on agarose and the portion >2 kb was cloned into the plasmid vector pcDNAII (Invitrogen). The original tranformation mix was frozen in glycerol without amplification and clones were arrayed directly from that glycerol stock. Library prepared by Dr. Joe Gatewood (Los Alamos National Laboratory). REF: "Gene Expression in an Abnormal Human Pregnancy", Joe M. Gatewood, Beckie Lobb, Cheryl Lemanski, Karen Denison, and Virgene Church.
||

NIH_MGC_10

NAME: NIH_MGC_10
LIB_ID: 1422
ORGANISM: human
ORGAN: placenta
TISSUE: choriocarcinoma cell line
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.4 kb. Library prepared by Life Technologies. Note: this is a NIH_MGC Library. '
||

NIH_MGC_11

NAME: NIH_MGC_11
LIB_ID: 1563
ORGANISM: human
ORGAN: placenta
TISSUE: choriocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-NCI
V_TYPE: phagemid
RE_5': SfiI
RE_3': SfiI
DESCR:
'Double-stranded cDNA was prepared from cell line RNA.
5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence:
5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence:
5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.65 kb (range 0.9-3.0 kb).
This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA).
Note: this is a NIH_MGC Library.
'
||

NIH_MGC_147

NAME: NIH_MGC_147
LIB_ID: 1964
ORGANISM: human
SEX: unknown
STAGE: placental
ORGAN: placenta
HOST: DH10B TonA
VECTOR: pBluescriptR
V_TYPE: phagemid
RE_5': SalI-XhoI
RE_3': BamHI
DESCR:
' Oligo-dT primed using primer 5''-TTTTTTTTTTTTTTTTVN-3'', size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library. '
||

NIH_MGC_148

NAME: NIH_MGC_148
LIB_ID: 1966
ORGANISM: human
STAGE: placental
ORGAN: placenta
TISSUE: pre-eclamptic placenta
HOST: DH10B TonA
VECTOR: pBluescriptR
V_TYPE: phagemid
RE_5': SalI-XhoI
RE_3': BamHI
DESCR:
' Oligo-dT primed using primer 5''-TTTTTTTTTTTTTTTTVN-3'', size-selected for average insert size 2.3 kb and normalized to ROT 5. This is a primary library enriched for full-length clones and constructed using the Cap-trapper method (Carninci, in preparation). Library constructed by M. Brownstein (NIMH/NHGRI, National Institutes of Health). Note: this is a NIH_MGC Library. '
||

NIH_MGC_21

NAME: NIH_MGC_21
LIB_ID: 1431
ORGANISM: human
ORGAN: placenta
TISSUE: choriocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_302

NAME: NIH_MGC_302
LIB_ID: 2268
ORGANISM: human
ORGAN: placenta
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PmeI site and the 3' end nearest the NotI site. Companion library NIH_MGC_303 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_303

NAME: NIH_MGC_303
LIB_ID: 2269
ORGANISM: human
ORGAN: placenta
HOST: DH10B TonA
VECTOR: pCR4-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR4-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the NotI site and the 3' end nearest the PmeI site. Companion library NIH_MGC_302 has clones in the opposite orientation. Every submitted sequence starts with the vector sequence (GAATTCGCCCTT) and ends with the vector sequence (AAGGGCGAATTC). Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_304

NAME: NIH_MGC_304
LIB_ID: 2290
ORGANISM: human
ORGAN: placenta
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the SpeI site and the 3' end nearest the PstI site. Companion library NIH_MGC_305 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_305

NAME: NIH_MGC_305
LIB_ID: 2291
ORGANISM: human
ORGAN: placenta
HOST: DH10B TonA
VECTOR: pCR-XL-TOPO
V_TYPE: plasmid
RE_5': TA cloning
RE_3': TA cloning
DESCR:
Clones were individually RT-PCR'd using primers directed against full-length RefSeq sequences. PCR products were TA-cloned into the pCR-XL-TOPO vector and inserts can be digested using EcoRI alone. Clones are oriented with the 5' end nearest the PstI site and the 3' end nearest the SpeI site. Companion library NIH_MGC_304 has clones in the opposite orientation. Library constructed and clones sequenced by the BCCA Genome Sciences Centre (Vancouver, Canada). Note: this is a Mammalian Gene Collection library.
||

NIH_MGC_79

NAME: NIH_MGC_79
LIB_ID: 1658
ORGANISM: human
ORGAN: placenta
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.3 kb (range 0.5-4.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

Soares 2NbHP8-9W

NAME: Soares 2NbHP8-9W
LIB_ID: 189
ORGANISM: human
STAGE: placental
ORGAN: placenta
TISSUE: two pooled
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' AACTGGAAGAATTCGCGGCCGCGATTTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Soares Nb2HP

NAME: Soares Nb2HP
LIB_ID: 77
ORGANISM: human
STAGE: placental
ORGAN: placenta
TISSUE: full-term placenta obtained at birth
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' AACTGGAAGAATTCGCGGCCGCAGGAATTTTTTTTTTTTTTTTTTT 3'], double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization. Library constructed by Bento Soares and M. Fatima Bonaldo.
||

Stratagene placenta (937225)

NAME: Stratagene placenta (937225)
LIB_ID: 61
ORGANISM: human
SEX: male
STAGE: fetal
ORGAN: placenta
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Caucasian. Average insert size: 1.2 kb; Uni-ZAP XR Vector; ~5' adaptor sequence: 5' GAATTCGGCACGAG 3' ~3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3'
||

Barstead HPL-RB4

NAME: Barstead HPL-RB4
LIB_ID: 1060
ORGANISM: human
SEX: male
STAGE: adult
AGE: 89
ORGAN: prostate
TISSUE: BPH
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer[5' TGTTACGAATCTGAAGTGGGAGCGGCCGCCCTTTTTTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors [5' AATTCGTCGACATC 3' and 5' GATGTCGACG 3'], digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library constructed by R. Barstead (Oklahoma Medical Research Foundation).
||

Barstead HPL-RB4.1

NAME: Barstead HPL-RB4.1
LIB_ID: 1295
ORGANISM: human
SEX: male
STAGE: adult
AGE: 89
ORGAN: prostate
TISSUE: BPH
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Gel-purification of HPL-RB4 to isolate clones with larger insertsize. Library constructed by Bob Barstead.
||

NCI_CGAP_Pr1

NAME: NCI_CGAP_Pr1
LIB_ID: 381
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: epithelium
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal prostate epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr10

NAME: NCI_CGAP_Pr10
LIB_ID: 466
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: invasive tumor
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive prostate tumor, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr11

NAME: NCI_CGAP_Pr11
LIB_ID: 467
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: epithelial cells
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal prostatic epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr15

NAME: NCI_CGAP_Pr15
LIB_ID: 489
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: epithelial cells
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal prostate epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size- selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr16

NAME: NCI_CGAP_Pr16
LIB_ID: 490
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: invasive tumor
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive prostate tumor cells, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr18

NAME: NCI_CGAP_Pr18
LIB_ID: 492
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: BPH, stroma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from prostate BPH, stroma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr2

NAME: NCI_CGAP_Pr2
LIB_ID: 382
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: intraepithelial premalignant neoplasia
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from preneoplastic intra-epithelial prostate neoplasia (low-grade), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr21

NAME: NCI_CGAP_Pr21
LIB_ID: 503
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: bulk tissue
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from normal prostate bulk tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAGTGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is not normalized. (Normalized version of this library is named NCI_CGAP_Pr22.) Library was constructed by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr22

NAME: NCI_CGAP_Pr22
LIB_ID: 504
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: bulk tissue
HOST: DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was prepared from normal prostate bulk tissue, and was then primed with a Not I - oligo(dT) primer: 5'-TGTTACCAATCTGAAGTGGGAGCGGCCGCAAGTGTTTTTTTTTTTTTTTTTTTTTTTTT-3'; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library is normalized, and was constructed by Bento Soares and M. Fatima Bonaldo. (Non-normalized version of this library is named NCI_CGAP_Pr21. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr23

NAME: NCI_CGAP_Pr23
LIB_ID: 529
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: pooled tumors
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Pooled prostate tumors. 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.2 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr24

NAME: NCI_CGAP_Pr24
LIB_ID: 601
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: invasive tumor cell line, HPV immortalized
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Invasive prostate tumor cell line (HPV immortalized). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.0 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr25

NAME: NCI_CGAP_Pr25
LIB_ID: 602
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: epithelial cell line, HPV immortalized
HOST: SOLR
VECTOR: pBluescript SK-
V_TYPE: phagemid
RE_5': EcoRI
RE_3': XhoI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Normal prostate epithelial cell line (HPV immortalized). 5' adaptor sequence: 5' GAATTCGGCACGAG 3' 3' adaptor sequence: 5' CTCGAGTTTTTTTTTTTTTTTTTT 3' Average insert size: 1.1 kb. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr28

NAME: NCI_CGAP_Pr28
LIB_ID: 1244
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: bulk tissue
HOST: GeneHogs DH10B
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
Plasmid DNA from the normalized library NCI_CGAP_Pr22 was prepared, and ss circles were made in vitro. Following HAP purification, this DNA was used as tracer in a subtractive hybridization reaction. The driver was PCR-amplified cDNAs from a pool of 5,000 clones made from the same library (cloneIDs 985608-986759, 1101192-1101959, and 1217928-1220615). Subtraction by Bento Soares and M. Fatima Bonaldo. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr3

NAME: NCI_CGAP_Pr3
LIB_ID: 383
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: invasive epithelial tumor
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive prostate tumor, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr4

NAME: NCI_CGAP_Pr4
LIB_ID: 384
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: intraepithelial neoplasia (high-grade)
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from prostate intraepithelial neoplasia (high-grade), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr4.1

NAME: NCI_CGAP_Pr4.1
LIB_ID: 540
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: intraepithelial neoplasia (high-grade)
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from prostatic intraepithelial neoplasia (high-grade), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr5

NAME: NCI_CGAP_Pr5
LIB_ID: 461
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: epithelial cells
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal prostatic epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr6

NAME: NCI_CGAP_Pr6
LIB_ID: 462
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: intraepithelial neoplasia (low-grade)
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from prostatic intraepithelial neoplasia (low-grade), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr7

NAME: NCI_CGAP_Pr7
LIB_ID: 463
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: intraepithelial neoplasia (low grade)
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from prostate intraepithelial neoplasia (high-grade), cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr8

NAME: NCI_CGAP_Pr8
LIB_ID: 464
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: invasive tumor
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from invasive prostate tumor, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Pr9

NAME: NCI_CGAP_Pr9
LIB_ID: 465
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: epithelial cells
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal prostatic epithelial cells, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 600 bp. Library made by D. Krizman, NIH. cDNA Library Preparation: David B. Krizman, Ph.D. Reference: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_RDF1

NAME: NCI_CGAP_RDF1
LIB_ID: 1442
ORGANISM: human
SEX: male
ORGAN: prostate
TISSUE: normal epithelium
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from normal prostate epithelium, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_RDF2

NAME: NCI_CGAP_RDF2
LIB_ID: 1443
ORGANISM: human
SEX: male
ORGAN: prostate
TISSUE: carcinoma
HOST: DH10B
VECTOR: pAMP10
V_TYPE: phagemid
RE_5': UDG
RE_3': UDG
DESCR:
mRNA made from prostatic carcinoma, cDNA made by oligo-dT priming. Non-directionally cloned into UDG sites. Size-selected on agarose gel, average insert size 500 bp. Primary library. cDNA Library Preparation: David B. Krizman, Ph.D. REFERENCE: Krizman et al. (1996) Cancer Research 56:5380-5383. Note: this is a NCI_CGAP Library.
||

NIH_MGC_111

NAME: NIH_MGC_111
LIB_ID: 1815
ORGANISM: human
SEX: male
ORGAN: prostate
TISSUE: prostate carcinoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_210

NAME: NIH_MGC_210
LIB_ID: 2216
ORGANISM: human
SEX: male
ORGAN: prostate
TISSUE: CNCAP(3)T-225 cell line
HOST: DH10B TonA
VECTOR: pT7T3D-PacI
V_TYPE: plasmid
RE_5': EcoRI
RE_3': NotI
DESCR:
1st strand cDNA was primed with a Not I - oligo(dT) primer [5' TGTTACCAATCTGAAGTGGGAGCGGCCGCCCCACTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library was constructed by Bento Soares and M. Fatima Bonaldo (University of Iowa). Note: this is a NIH_MGC Library.
||

NIH_MGC_40

NAME: NIH_MGC_40
LIB_ID: 1735
ORGANISM: human
SEX: male
STAGE: adult
ORGAN: prostate
TISSUE: prostate carcinoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_60

NAME: NIH_MGC_60
LIB_ID: 1588
ORGANISM: human
SEX: male
ORGAN: prostate
TISSUE: adenocarcinoma cell line
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' Double-stranded cDNA was prepared from cell line RNA. 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.5 kb (range 0.9-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_83

NAME: NIH_MGC_83
LIB_ID: 1653
ORGANISM: human
SEX: male
ORGAN: prostate
HOST: GeneHogs DH10B
VECTOR: pDNR-LIB
V_TYPE: plasmid
RE_5': SfiI
RE_3': SfiI
DESCR:
' 5'' and 3'' adaptors were used in cloning as follows: 5'' adaptor sequence: 5''-CACGGCCATTATGGCC-3'' and 3'' adaptor sequence: 5''-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3'' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.4 kb (range 0.5-4.0 kb). 14/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a NIH_MGC Library. '
||

NIH_MGC_91

NAME: NIH_MGC_91
LIB_ID: 1727
ORGANISM: human
SEX: male
ORGAN: prostate
TISSUE: adenocarcinoma cell line
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
'Cloned unidirectionally; oligo-dT primed. Average insert size 1.4 kb. Library enriched for full-length clones and constructed by Life Technologies. Note: this is a NIH_MGC Library. '
||

NCI_CGAP_Mel15

NAME: NCI_CGAP_Mel15
LIB_ID: 1350
ORGANISM: human
ORGAN: skin
TISSUE: malignant melanoma, metastatic to lymph node
HOST: DH10B
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Skn1

NAME: NCI_CGAP_Skn1
LIB_ID: 1591
ORGANISM: human
ORGAN: skin
TISSUE: 4 pooled samples
HOST: DH10B TonA
VECTOR: pCMV-SPORT6
V_TYPE: phagemid
RE_5': SalI
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.03kb. Library constructed by Life Technologies, cat # 11132-018. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Skn3

NAME: NCI_CGAP_Skn3
LIB_ID: 1743
ORGANISM: human
ORGAN: skin
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.ccdb
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NCI_CGAP_Skn4

NAME: NCI_CGAP_Skn4
LIB_ID: 1744
ORGANISM: human
ORGAN: skin
TISSUE: squamous cell carcinoma
HOST: DH10B TonA
VECTOR: pCMV-SPORT6.ccdb
V_TYPE: phagemid
RE_5': EcoRV
RE_3': NotI
DESCR:
Cloned unidirectionally. Primer: Oligo dT. Average insert size 1.5kb. Library constructed by Life Technologies. Note: this is a NCI_CGAP Library.
||

NIH_MGC_112

NAME: NIH_MGC_112
LIB_ID: 1816
ORGANISM: human
ORGAN: skin
TISSUE: melanotic melanoma, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_20

NAME: NIH_MGC_20
LIB_ID: 1430
ORGANISM: human
ORGAN: skin
TISSUE: melanotic melanoma cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: plasmid
RE_5': EcoRI
RE_3': XhoI
DESCR:
'cDNA made by oligo-dT priming. Directionally cloned into EcoRI/XhoI sites using the following 5'' adaptor: GGCACGAG(G). Size-selected >500bp for average insert size 1.8kb. Library constructed by Ling Hong in the laboratory of Gerald M. Rubin (University of California, Berkeley) using ZAP-cDNA synthesis kit (Stratagene) and Superscript II RT (Life Technologies). Note: this is a NIH_MGC Library. '
||

NIH_MGC_41

NAME: NIH_MGC_41
LIB_ID: 1736
ORGANISM: human
ORGAN: skin
TISSUE: melanoma, amelanotic, cell line
HOST: GeneHogs DH10B
VECTOR: pOTB7
V_TYPE: pla